| 1. |
|
|
| 2. |
- Pereira, Fabio M., et al.
(författare)
-
Hybrid Microfluidic Platform for Multifactorial Analysis Based on Electrical Impedance, Refractometry, Optical Absorption and Fluorescence
- 2016
-
Ingår i: Micromachines. - : MDPI AG. - 2072-666X. ; 7:10
-
Tidskriftsartikel (refereegranskat)abstract
- This paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead counting.
|
|
| 3. |
- Rasplus, Jean Yves, et al.
(författare)
-
A first phylogenomic hypothesis for Eulophidae (Hymenoptera, Chalcidoidea)
- 2020
-
Ingår i: Journal of Natural History. - : Informa UK Limited. - 0022-2933 .- 1464-5262. ; 54:9-12, s. 597-609
-
Tidskriftsartikel (refereegranskat)abstract
- Eulophidae is a hyper-diverse family of chalcidoid wasps with 324 genera, about 5300 described species and probably thousands of others to be described. Until now, the absence of unequivocal morphological apomorphies and the low resolution provided by the handful of Sanger sequenced genes have hampered the reconstruction of phylogenetic relationships within the family. Here, we used ultra-conserved elements and their flanking regions to resolve relationships among 84 species of eulophids included in 63 genera representing all subfamilies and most tribes, plus 15 outgroups. Our analyses recover all traditional Eulophidae subfamilies and tribes with high support and globally agree with the traditional classification of the family. Our results confirm that Eulophinae + Tetrastichinae is the sister group of (Opheliminae + Entiinae) + Entedoninae. At the generic level, our analyses provide high support for intergeneric relationships for which morphology and Sanger markers previously failed to provide resolution. Our results also confirm that Trisecodes does not group with Eulophidae and may not belong to this family; however, its correct classification still awaits a large-scale phylogenomic hypothesis for Chalcidoidea. This work opens new avenues towards a better understanding of the evolutionary history, biogeography and evolution of host–parasitoid associations in this hyper-diverse family of chalcidoid wasps.
|
|
