| 1. |
- Hemalatha, Thiagarajan, et al.
(författare)
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Fabrication and characterization of dual acting oleyl chitosan functionalised iron oxide/gold hybrid nanoparticles for MRI and CT imaging.
- 2018
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier BV. - 0141-8130 .- 1879-0003. ; 112, s. 250-257
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Tidskriftsartikel (refereegranskat)abstract
- Bionanocomposites fabricated using metal nanoparticles serve a wide range of biomedical applications viz., site targeted drug delivery, imaging etc. Theranostics emerge as an important field of science, which focuses on the use of single entity for both disease diagnosis and treatment. The present work aimed at designing a multifunctional nanocomposite comprising of iron/gold hybrid nanoparticles, coated with oleyl chitosan and conjugated with methotrexate. The HR-TEM images revealed the spherical nature of the composite, while it's nontoxic and biocompatible property was proved by the MTT assay in NIH 3T3 cells and hemolysis assay. Though the VSM results exhibited the magnetic property, the MRI phantom images and X-ray contrast images demonstrated the potential of the composite to be used as contrast agent. Thus the prepared nanocomposite possess good cytocompatibility, magnetic property and also high X-ray attenuation, wherein it could serve as a novel platform for both MRI and CT diagnosis, as well as drug conjugation could aid in targeted drug delivery.
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| 2. |
- Pathmanapan, Srinivetha, et al.
(författare)
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Fibrin hydrogel incorporated with graphene oxide functionalized nanocomposite scaffolds for bone repair - In vitro and in vivo study
- 2020
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Ingår i: Nanomedicine. - : ELSEVIER. - 1549-9634 .- 1549-9642. ; 29
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Tidskriftsartikel (refereegranskat)abstract
- Conventional bone repair therapies like the autologous and allogenic bone grafts have failed to meet challenges in bone reconstruction along with complications. Tissue engineering (TE) has emerged as a developing treatment regimen in regenerating damaged tissues rather than replacing them. In TE, biomaterials act as template for damaged tissues and function as artificial extracellular matrix (ECM), facilitating new tissue formation. Since single type biomaterial has unsuccessful regeneration properties, focus on using composites of natural and synthetic biomaterials is encouraged. In the current study, we have evaluated the potential of a graphene-based nano-composite scaffold as a biomaterial to enhance bone tissue regeneration. The findings demonstrate that the scaffold with Graphene oxide (GO) exhibits enhanced levels of biocompatibility, alkaline phosphatase activity, and calcium deposits, thereby emphasizing the hypothesis that fabricated nanocomposite scaffolds are promising osteoinductive products for bone repair/regeneration. (C) 2020 Elsevier Inc. All rights reserved.
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| 3. |
- Periyathambi, Prabu, et al.
(författare)
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Activatable MRI probes for the specific detection of bacteria
- 2021
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 413:30, s. 7353-7362
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Tidskriftsartikel (refereegranskat)abstract
- Activatable fluorescent probes have been successfully used as molecular tools for biomedical research in the last decades. Fluorescent probes allow the detection of molecular events, providing an extraordinary platform for protein and cellular research. Nevertheless, most of the fluorescent probes reported are susceptible to interferences from endogenous fluorescence (background signal) and limited tissue penetration is expected. These drawbacks prevent the use of fluorescent tracers in the clinical setting. To overcome the limitation of fluorescent probes, we and others have developed activatable magnetic resonance probes. Herein, we report for the first time, an oligonucleotide-based probe with the capability to detect bacteria using magnetic resonance imaging (MRI). The activatable MRI probe consists of a specific oligonucleotide that targets micrococcal nuclease (MN), a nuclease derived from Staphylococcus aureus. The oligonucleotide is flanked by a superparamagnetic iron oxide nanoparticle (SPION) at one end, and by a dendron functionalized with several gadolinium complexes as enhancers, at the other end. Therefore, only upon recognition of the MRI probe by the specific bacteria is the probe activated and the MRI signal can be detected. This approach may be widely applied to detect bacterial infections or other human conditions with the potential to be translated into the clinic as an activatable contrast agent.
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| 4. |
- Periyathambi, Prabu, et al.
(författare)
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Development of water-soluble curcumin grafted magnetic nanoparticles for enhancing bioavailability, fluorescence, and magnetic resonance imaging activity
- 2021
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Ingår i: Materials letters (General ed.). - : ELSEVIER. - 0167-577X .- 1873-4979. ; 294
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Tidskriftsartikel (refereegranskat)abstract
- The chemo-fluorescent drug curcumin (CUR) is a safe and effective anticancer agent. The primary limitation of CUR is its poor solubility in water, which limits its bioavailability. We have developed an efficient chemotherapeutic and amorphous magneto-fluorescent CUR grafted magnetic nanoparticles (CUR-MAG NPs) using wet chemical process. The CUR grafting of MAG NPs improved the solubility, bioavailability, and internalization activities of CUR. CUR-MAG NPs can be used as a novel platform for in vivo biomedical applications. (c) 2021 Elsevier B.V. All rights reserved.
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| 5. |
- Periyathambi, Prabu, et al.
(författare)
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Green biosynthesis and characterization of fibrin functionalized iron oxide nanoparticles with MRI sensitivity and increased cellular internalization
- 2014
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Ingår i: Materials Chemistry and Physics. - : Elsevier. - 0254-0584 .- 1879-3312. ; 148:3, s. 1212-1220
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Tidskriftsartikel (refereegranskat)abstract
- Developing novel contrast agents for efficient MRI sensitivity is very essential for early diagnosis of cancer. In the present work, we have prepared novel, eco-friendly, non-toxic iron oxide nanoparticles (IONPs) as efficient MRI contrast agents. Earth abundant magnetite sand was used for the preparation of IONPs using acid leaching method. Further, co-precipitation method was used to obtain fibrin functionalized iron nanoparticles (F-IONPs). The nanoparticles were crystalline in nature with average size distribution values as 15 nm and 35 nm for IONPs and F-IONPs respectively. Haemolysis and MTT assay proved the biocompatibility of the prepared nanoparticles with their distribution across the cytoplasm and nucleus in NIH 3T3 cells. Moreover, MRI and VSM results revealed the magnetic property of F-IONPs, with the magnetic saturation value of 51.7 emu g−1. Thus, F-IONPs with good cytocompatibility and high magnetic property can serve as a novel platform for enhanced MRI sensitivity and drug delivery.
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| 6. |
- Periyathambi, Prabu, et al.
(författare)
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Macrophages mediated diagnosis of rheumatoid arthritis using fibrin based magnetic nanoparticles as MRI contrast agents.
- 2017
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Ingår i: Biochimica et Biophysica Acta. - : Elsevier BV. - 0006-3002 .- 1878-2434 .- 0304-4165. ; 1861:1 Pt A, s. 2992-3001
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: A variety of bioimaging tools assists in the diagnosis and evaluation of rheumatoid arthritis (RA) and other osteoarthritis. However, detection of RA in the early stages by targeting its macrophages with suitable contrast agents will help in arresting the progression of the disease.METHODS: In the present study, we investigated the effectiveness of using magnetic fibrin nanoparticles (MFNPs) conjugated with folic acid (FA-MFNPs) as a specific contrast agent to target the activated macrophages, which overexpress the folate receptors (FR) in the knee joints of rats with antigen-induced arthritis (AIA).RESULTS: FA-MFNPs were spherical with an average size of 18.3±1.6nm. In vitro studies have shown effective internalization of FA-MFNPs into the Raw264.7 macrophage cells. In vivo studies were carried out by injecting FA-MFNPs intravenously into the arthritic rats. The results showed enhanced MR imaging in the synovium of arthritic joints. Prussian blue histological staining confirmed uptake of FA-MFNPs by macrophages in the synovial tissue.CONCLUSION: The animal experiment results indicate that FA-MFNPs can be used as a specific MRI contrast agent in identifying phagocytic active macrophages in the synovial joints.GENERAL SIGNIFICANCE: Blood is the precursor source for synthesising the fibrin-based iron oxide (magnetic) nanoparticles (MFNPs) with diameters between 12 and 15nm. It has excellent superparamagnetic behaviour, biocompatibility, osteogenic potency, hemocompatibility, and biodegradable properties. MFNPs-based nanocomposites might be a promising contrast agent for bioimaging.
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| 7. |
- Periyathambi, Prabu, et al.
(författare)
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Osteogenic potency of magnetic fibrin nanoparticles—A novel perspective in bone tissue engineering
- 2015
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Ingår i: Materials letters (General ed.). - : Elsevier BV. - 0167-577X .- 1873-4979. ; 139, s. 108-111
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Tidskriftsartikel (refereegranskat)abstract
- In the present study, goat blood has been used as the starting material for the preparation of novel magnetic fibrin nanoparticles (MAG–FNPs) in tissue engineering. The synthesised nanoparticles were characterized by X-ray diffractometer (XRD), transmittance electron microscope (TEM), scanning electron microscope (SEM), Fourier Transform Infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX) and vibrating sample magnetometer (VSM) analysis. The osteogenic potential of MAG–FNPs was evaluated by performing cell viability assay and quantifying alkaline phosphatase (ALP) levels using Saos-2 cells. The results obtained suggested that MAG–FNPs could serve as promising biomaterial for bone tissue engineering.
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| 8. |
- Periyathambi, Prabu, et al.
(författare)
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Time-dependent biodistribution, clearance and biocompatibility of magnetic fibrin nanoparticles : an in vivo study.
- 2015
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Ingår i: Nanoscale. - : Royal Society of Chemistry. - 2040-3364 .- 2040-3372. ; 7:21, s. 9676-9685
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Tidskriftsartikel (refereegranskat)abstract
- Recently, bioretention and toxicity of injected nanoparticles in the body has drawn much attention in biomedical research. In the present study, 5 mg Fe per kg body weight of magnetic fibrin nanoparticles (MFNPs) were injected into mice intravenously and investigated for their blood clearance profile, biodistribution, haematology and pathology studies for a time period of 28 days. Moderately long circulation of MFNPs in blood was observed with probable degradation and excretion into the bloodstream via monoatomic iron forms. Inductively coupled plasma optical emission spectrometry (ICP-OES) and Prussian blue staining results showed increased accumulation of MFNPs in the liver, followed by spleen and other organs. Body weight, spleen/thymus indexes, haematology, serum biochemistry and histopathology studies demonstrated that MFNPs were biocompatible. These results suggest the feasibility of using MFNPs for drug delivery and imaging applications.
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| 9. |
- Vedakumari, Weslen S, et al.
(författare)
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Fibrin nanoparticles as Possible vehicles for drug delivery.
- 2013
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Ingår i: Biochimica et Biophysica Acta. - : Elsevier BV. - 0006-3002 .- 1878-2434 .- 0304-4165. ; 1830:8, s. 4244-4253
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Several issues have been raised emphasizing the harmful toxic effects of metal nanoparticles towards biological systems. Search of biological nanoparticles with excellent biocompatibility and bioavailability could address this problem.METHODS: Fibrin nanoparticles (FNP) were prepared using a novel technique and characterized for their physico-chemical properties. In vitro studies were performed to examine cytotoxicity and cellular uptake of FNP. Innate immune response to FNP was studied by (i) estimating in vitro generation of complement split products, C3a and C4d and (ii) in vivo expression of pro-inflammatory cytokines, TNF-α, IL-1 and IL-6. In vivo biodistribution study was carried out by intravenous administration of FITC-labelled FNP in mice.RESULTS: FNP were spherical with size ranging from 25 to 28nm. In vitro studies proved the biocompatibility of the nanoparticles, with their distribution across the cytoplasm and nucleus of treated cells. Complement activation studies showed insignificant increase in the level of C3a when compared with positive control. RT-PCR results revealed significant upregulation of TNF-α and downregulation of IL-6 cytokines after 6h of FNP administration. In vivo biodistribution studies showed moderate blood circulation time, with predominant distribution of nanoparticles in the liver followed by the lungs, kidney and spleen. Haematology, serum biochemistry, and histopathology analyses demonstrated that FNP were non-toxic.CONCLUSION: Owing to their small size, low cost, ease of preparation and excellent biocompatibility, FNP might be a promising novel material for drug delivery applications.GENERAL SIGNIFICANCE: Our results demonstrate the safe and promising use of FNP for biomedical applications.
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