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- Diaconu, Iulia, et al.
(författare)
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Immune Response Is an Important Aspect of the Antitumor Effect Produced by a CD40L-Encoding Oncolytic Adenovirus
- 2012
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Ingår i: Cancer Research. - 0008-5472 .- 1538-7445. ; 72:9, s. 2327-2338
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Tidskriftsartikel (refereegranskat)abstract
- Oncolytic adenovirus is an attractive platform for immunotherapy because virus replication is highly immunogenic and not subject to tolerance. Although oncolysis releases tumor epitopes and provides costimulatory danger signals, arming the virus with immunostimulatory molecules can further improve efficacy. CD40 ligand (CD40L, CD154) induces apoptosis of tumor cells and triggers several immune mechanisms, including a T-helper type 1 (TH1) response, which leads to activation of cytotoxic T cells and reduction of immunosuppression. In this study, we constructed a novel oncolytic adenovirus, Ad5/3-hTERT-E1A-hCD40L, which features a chimeric Ad5/3 capsid for enhanced tumor transduction, a human telomerase reverse transcriptase (hTERT) promoter for tumor selectivity, and human CD40L for increased efficacy. Ad5/3-hTERT-E1A-hCD40L significantly inhibited tumor growth in vivo via oncolytic and apoptotic effects, and (Ad5/3-hTERT-E1A-hCD40L)–mediated oncolysis resulted in enhanced calreticulin exposure and HMGB1 and ATP release, which were suggestive of immunogenicity. In two syngeneic mouse models, murine CD40L induced recruitment and activation of antigen-presenting cells, leading to increased interleukin-12 production in splenocytes. This effect was associated with induction of the TH1 cytokines IFN-γ, RANTES, and TNF-α. Tumors treated with Ad5/3-CMV-mCD40L also displayed an enhanced presence of macrophages and cytotoxic CD8+ T cells but not B cells. Together, our findings show that adenoviruses coding for CD40L mediate multiple antitumor effects including oncolysis, apoptosis, induction of T-cell responses, and upregulation of TH1 cytokines.
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| 2. |
- Alisaari, Jenni, et al.
(författare)
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Mother tongue education in four Nordic countries : problem, right or resource?
- 2023
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Ingår i: Apples - Journal of Applied Language Studies. - : University of Jyväskylä. - 1457-9863. ; 17:2, s. 52-72
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Tidskriftsartikel (refereegranskat)abstract
- Jenni Alisaari, University of Stockholm, Sweden & University of Turku, Finland Line Møller Daugaard, VIA University College, Denmark Joke Dewilde University of Oslo, NorwayRaisa Harju-Autti, Tampere University, Finland Leena Maria Heikkola, Åbo Akademi University, FinlandJonas Yassin Iversen, Inland Norway University of Applied Sciences Niina Kekki, University of Turku, Finland Sari Pesonen, University of Stockholm, SwedenAnne Reath Warren, Uppsala University, Sweden Boglárka Straszer, Dalarna University, Sweden Maija Yli-Jokipii, Tampere University, FinlandThe Declaration of a Nordic Language Policy stipulates that all Nordic residents have the right to preserve and develop their mother tongue and their national minority languages. Hence, this article investigates the question of mother tongue education for linguistic minority students. Through four ‘telling cases’, the article explores how four Nordic countries, Denmark, Finland, Norway, and Sweden, orient towards mother tongues, Indigenous and national minority languages in their educational policies. Drawing on Ruíz’ (1984) framework of orientations in language planning, we investigate the following question: In what ways are mother tongues framed as rights, resources, or problems in four telling cases of educational policy in Denmark, Finland, Norway and Sweden? The analysis of the telling cases shows that although all four countries provide various forms of mother tongue education, thus apparently aligning with the intentions in the Declaration of a Nordic Language Policy, there are important differences between the provisions. Nevertheless, across the four countries, the official national languages are placed at the top of a language ideological hierarchy. The official national languages are followed by national minority languages as mother tongues. These languages are awarded rights but are not considered resources for the whole population (e.g., Ruíz, 1984). The Danish telling case inserts a supranational layer in the hierarchy, namely mother tongues with status as official languages in the European Union. The hierarchy of mother tongues thus reflects how some types of mother tongues are more readily granted rights and considered to be resources than others.
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| 3. |
- Fortino, Vittorio, et al.
(författare)
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Machine-learning-driven biomarker discovery for the discrimination between allergic and irritant contact dermatitis
- 2020
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 117:52, s. 33474-33485
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Tidskriftsartikel (refereegranskat)abstract
- Contact dermatitis tremendously impacts the quality of life of suffering patients. Currently, diagnostic regimes rely on allergy testing, exposure specification, and follow-up visits; however, distinguishing the clinical phenotype of irritant and allergic contact dermatitis remains challenging. Employing integrative transcriptomic analysis and machine-learning approaches, we aimed to decipher disease-related signature genes to find suitable sets of biomarkers. A total of 89 positive patch-test reaction biopsies against four contact allergens and two irritants were analyzed via microarray. Coexpression network analysis and Random Forest classification were used to discover potential biomarkers and selected biomarker models were validated in an independent patient group. Differential gene-expression analysis identified major gene-expression changes depending on the stimulus. Random Forest classification identified CD47, BATF, FASLG, RGS16, SYNPO, SELE, PTPN7, WARS, PRC1, EXO1, RRM2, PBK, RAD54L, KIFC1, SPC25, PKMYT, HISTH1A, TPX2, DLGAP5, TPX2, CH25H, and IL37 as potential biomarkers to distinguish allergic and irritant contact dermatitis in human skin. Validation experiments and prediction performances on external testing datasets demonstrated potential applicability of the identified biomarker models in the clinic. Capitalizing on this knowledge, novel diagnostic tools can be developed to guide clinical diagnosis of contact allergies.
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| 7. |
- Lainio, Jarmo, et al.
(författare)
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»Önskar att jag hade fått hjälp att lära min son finska.» : Finskans öden i skenet av 2000-talets utbildnings- och minoritetspolitiska utveckling i Sverige
- 2021
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Ingår i: Språk och stil. - : Uppsala University. - 1101-1165 .- 2002-4010. ; NF 31:1, s. 44-74
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Tidskriftsartikel (refereegranskat)abstract
- This article aims to establish the ways in which different aspects of the Swedish national minority language policy have facilitated or hindered the fulfilling of the aims to revitalize these languages. Finnish is used as a test case, and we apply a two-step approach to analyzing the development of its use during the 2000s: we adapt the COD model (Capacity, Opportunity, Desire), proposed by Grin in the early 1990s, and apply it at three societal levels: macro, meso and micro. The macro level covers national policies, laws and other regulations. The meso level is represented by the adoption and implementation of these locally. The micro level consists of linguistic biographies by informants of a bilingual project started in 1991, and revisited in 2013. Education in or of Finnish as an example of language policy is central to the study, although other fields are considered.Our analysis shows that policies established at the national level recur in different forms at the meso and micro levels. Although there is an overlap between these societal levels, there is a discrepancy between legislation and practice. The dimension of capacity in Finnish seems to account for much of the development in language use, compared to opportunity and desire. The COD model, in combination with its multilevel adaptation, is a functional, analytic option for the study of language policy decisions and their effect on language use patterns. It therefore could also be adapted to the other national minority languages of Sweden.
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| 10. |
- Pesonen, Sari
(författare)
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Karkkitalosta hammaslääkäriin : suomea Ruotsissa
- 2009
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Ingår i: Karjalanpaistista kaksoiskonsonanttiin. - Helsinki : Kansainvälisen liikkuvuuden ja yhteistyön keskus CIMO. - 9789518052886 ; , s. 142-149
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Bokkapitel (populärvet., debatt m.m.)
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