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- Bryant, Helen E, et al.
(författare)
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PARP is activated at stalled forks to mediate Mre11-dependent replication restart and recombination.
- 2009
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Ingår i: The EMBO journal. - : Wiley. - 1460-2075 .- 0261-4189. ; 28:17, s. 2601-15
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Tidskriftsartikel (refereegranskat)abstract
- If replication forks are perturbed, a multifaceted response including several DNA repair and cell cycle checkpoint pathways is activated to ensure faithful DNA replication. Here, we show that poly(ADP-ribose) polymerase 1 (PARP1) binds to and is activated by stalled replication forks that contain small gaps. PARP1 collaborates with Mre11 to promote replication fork restart after release from replication blocks, most likely by recruiting Mre11 to the replication fork to promote resection of DNA. Both PARP1 and PARP2 are required for hydroxyurea-induced homologous recombination to promote cell survival after replication blocks. Together, our data suggest that PARP1 and PARP2 detect disrupted replication forks and attract Mre11 for end processing that is required for subsequent recombination repair and restart of replication forks.
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- Grewe, E. W., et al.
(författare)
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Studies on the double-beta decay nucleus Zn-64 using the (d,He-2) reaction
- 2008
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Ingår i: Physical Review C - Nuclear Physics. - 2469-9985 .- 2469-9993. ; 77:6
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Tidskriftsartikel (refereegranskat)abstract
- The (d,He-2) charge-exchange reaction on the double-beta decay (beta beta) nucleus Zn-64 has been studied at an incident energy of 183 MeV. The two protons in the S-1(0) state (indicated as He-2) were both momentum analyzed and detected simultaneously by the BBS magnetic spectrometer and its position-sensitive detector. He-2 spectra with a resolution of about 115 keV (FWHM) have been obtained allowing identification of many levels in the residual nucleus Cu-64 with high precision. Zn-64 is one of the rare cases undergoing a beta beta decay in beta(+) direction. In the experiment presented here, Gamow-Teller (GT(+)) transition strengths have been extracted. Together with the GT(-) transition strengths from Ni-64(He-3,t) data to the same intermediate nucleus Cu-64, the nuclear matrix elements of the beta beta decay of Zn-64 have been evaluated. Finally, the GT(+/-) distributions are compared with shell-model calculations and a critical assessment is given of the various residual interactions presently employed for the pf shell.
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- Risal, Sanjiv, et al.
(författare)
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Prenatal androgen exposure and transgenerational susceptibility to polycystic ovary syndrome
- 2019
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Ingår i: Nature Medicine. - : Nature Publishing Group. - 1078-8956 .- 1546-170X. ; 25:12, s. 1894-1904
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Tidskriftsartikel (refereegranskat)abstract
- How obesity and elevated androgen levels in women with polycystic ovary syndrome (PCOS) affect their offspring is unclear. In a Swedish nationwide register-based cohort and a clinical case-control study from Chile, we found that daughters of mothers with PCOS were more likely to be diagnosed with PCOS. Furthermore, female mice (F0) with PCOS-like traits induced by late-gestation injection of dihydrotestosterone, with and without obesity, produced female F1-F3 offspring with PCOS-like reproductive and metabolic phenotypes. Sequencing of single metaphase II oocytes from F1-F3 offspring revealed common and unique altered gene expression across all generations. Notably, four genes were also differentially expressed in serum samples from daughters in the case-control study and unrelated women with PCOS. Our findings provide evidence of transgenerational effects in female offspring of mothers with PCOS and identify possible candidate genes for the prediction of a PCOS phenotype in future generations.
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