SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Picaud S) "

Sökning: WFRF:(Picaud S)

  • Resultat 1-10 av 17
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  •  
2.
  • Brodelius, M, et al. (författare)
  • Immunolocalization of the saposin-like insert of plant aspartic proteinases exhibiting saposin C activity. Expression in young flower tissues and in barley seeds
  • 2005
  • Ingår i: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 125:4, s. 405-418
  • Tidskriftsartikel (refereegranskat)abstract
    • The plant- specific insert ( PSI) of cypro11 gene- encoding cyprosin, an aspartic proteinase from Cynara cardunculus, has been cloned by polymerase chain reaction ( PCR) into a bacterial expression vector. A rearranged form of this PSI in which the N- and C- terminal sequences were permutated to make it more similar to the structural arrangement observed in saposins was also cloned and expressed in the same system. The biological activities of the two purified recombinant proteins were compared to those of human saposins B and C. The proteins showed similar activity to saposin C, i. e. capacity to activate human glucosylceramidase. At a concentration of 5 mu M, wild- type PSI, saposin C, and rearranged PSI activated human glucosylceramidase two-, three-, and five- fold, respectively. The K-m for 4- methylumbelliferyl beta-glucopyranoside was around 7 mM in the presence of any of the three activators ( 5 mM). The neurotropic activity using NS20Y cells and lipid- binding properties of the plant recombinant proteins were tested. The two plant proteins showed lipid- binding properties similar to those of saposins but did not have any effect on neurite outgrowth. Immunolocalization of PSI showed its expression in protective tissues in flower meristem - protodermis, in C. cardunculus and embryonic root cap and coleorhiza in mature barley grains - as well as husk, pericarp, and the aleurone layer. Possible biological functions suggested for the plant homologue to saposins besides the general activation of enzymes involved in lipid metabolism would be involvement in plant defence.
  •  
3.
  •  
4.
  •  
5.
  •  
6.
  •  
7.
  •  
8.
  •  
9.
  •  
10.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-10 av 17

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy