| 1. |
- Refaat, Doaa, et al.
(författare)
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Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies-Synthesis and Applications
- 2019
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Ingår i: International Journal of Molecular Sciences. - : MDPI. - 1661-6596 .- 1422-0067. ; 20:24, s. 1-21
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Forskningsöversikt (refereegranskat)abstract
- Materials that can mimic the molecular recognition-based functions found in biology are a significant goal for science and technology. Molecular imprinting is a technology that addresses this challenge by providing polymeric materials with antibody-like recognition characteristics. Recently, significant progress has been achieved in solving many of the practical problems traditionally associated with molecularly imprinted polymers (MIPs), such as difficulties with imprinting of proteins, poor compatibility with aqueous environments, template leakage, and the presence of heterogeneous populations of binding sites in the polymers that contribute to high levels of non-specific binding. This success is closely related to the technology-driven shift in MIP research from traditional bulk polymer formats into the nanomaterial domain. The aim of this article is to throw light on recent developments in this field and to present a critical discussion of the current state of molecular imprinting and its potential in real world applications.
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| 2. |
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| 3. |
- Kyprianou, Dimitris, et al.
(författare)
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New reactive polymer for protein immobilisation on sensor surfaces
- 2009
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Ingår i: Biosensors & bioelectronics. - : Elsevier Science B.V., Amsterdam.. - 0956-5663 .- 1873-4235. ; 24:5, s. 1365-1371
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Tidskriftsartikel (refereegranskat)abstract
- Immobilisation of biorecognition elements on transducer surfaces is a key step in the development of biosensors. The immobilisation needs to be fast, cheap and most importantly should not affect the biorecognition activity of the immobilised receptor. A novel protocol for the covalent immobilisation of biomolecules containing primary amines using an inexpensive and simple polymer is presented. This tridimensional (3D) network leads to a random immobilisation of antibodies on the polymer and ensures the availability of a high percentage of antibody binding sites. The reactivity of the polymer is based on the reaction between primary amines and thioacetal groups included in the polymer network. These functional groups (thioacetal) do not need any further activation in order to react with proteins, making it attractive for sensor fabrication. The novel polymer also contains thiol derivative groups (disulphide groups or thioethers) that promote self-assembling on a metal transducer surface. For demonstration purposes the polymer was immobilised on Au Biacore chips. The resulting polymer layer was characterised using contact angle meter, atomic force microscopy (AFM) and ellipsometry. A general protocol suitable for the immobilisation of bovine serum albumin (BSA), enzymes and antibodies such as polyclonal anti-microcystin-LR antibody and monoclonal anti-prostate specific antigen (anti-PSA) antibody was then optimised. The affinity characteristics of developed immunosensors were investigated in reaction with microcystin-LR, and PSA. The calculated detection limit for analytes depended on the properties of antibodies. The detection limit for microcystin-LR was 10 ng mL(-1) and for PSA 0.01 ng mL(-1). The non-specific binding of analytes to synthesised polymers was very low. The polymer-coated chips were stored for up to 2 months without any noticeable deterioration in their ability to react with proteins. These findings make this new polymer very promising for the development of low-cost, easy to prepare and sensitive biosensors. (C) 2008 Elsevier B.V. All rights reserved.
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| 4. |
- Uludag, Yildiz, et al.
(författare)
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Piezoelectric sensors based on molecular imprinted polymers for detection of low molecular mass analytes
- 2007
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Ingår i: The FEBS Journal. - : Blackwell Publishing. - 1742-464X .- 1742-4658. ; 274:21, s. 5471-5480
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Forskningsöversikt (refereegranskat)abstract
- Biomimetic recognition elements employed for the detection of analytes are commonly based on proteinaceous affibodies, immunoglobulins, single-chain and single-domain antibody fragments or aptamers. The alternative supra-molecular approach using a molecularly imprinted polymer now has proven utility in numerous applications ranging from liquid chromatography to bioassays. Despite inherent advantages compared with biochemical/biological recognition (which include robustness, storage endurance and lower costs) there are few contributions that describe quantitative analytical applications of molecularly imprinted polymers for relevant small molecular mass compounds in real-world samples. There is, however, significant literature describing the use of low-power, portable piezoelectric transducers to detect analytes in environmental monitoring and other application areas. Here we review the combination of molecularly imprinted polymers as recognition elements with piezoelectric biosensors for quantitative detection of small molecules. Analytes are classified by type and sample matrix presentation and various molecularly imprinted polymer synthetic fabrication strategies are also reviewed.
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| 5. |
- Berti, Francesca, et al.
(författare)
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Quasi-monodimensional polyaniline nanostructures for enhanced molecularly imprinted polymer-based sensing
- 2010
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Ingår i: Biosensors & bioelectronics. - : Elsevier Science B.V., Amsterdam.. - 0956-5663 .- 1873-4235. ; 26:2, s. 497-503
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Tidskriftsartikel (refereegranskat)abstract
- Recent advances in nanotechnology have allowed significant progress in utilising cutting-edge techniques associated with nanomaterials and nano-fabrication to expand the scope and capability of biosensors to a new level of novelty and functionality. The aim of this work was the development and characterisation of conductive polyaniline (PANI) nanostructures for applications in electrochemical biosensing. We explore a simple, inexpensive and fast route to grow PANI nanotubes, arranged in an ordered structure directly on an electrode surface, by electrochemical polymerisation using alumina nanoporous membranes as a nano-mould. The deposited nanostructures have been characterised electrochemically and morphologically prior to grafting with a molecularly imprinted polymer (MIP) receptor in order to create a model sensor for catechol detection. In this way, PANI nanostructures resulted in a conductive nanowire system which allowed direct electrical connection between the electrode and the synthetic receptor (MIP). To our knowledge, this is the first example of integration between molecularly imprinted polymers and PANI nanostructured electrodes. The advantages of using nanostructures in this particular biosensing application have been evaluated by comparing the analytical performance of the sensor with an analogous non-nanostructured MIP-sensor for catechol detection that was previously developed. A significantly lower limit of detection for catechol has been obtained (29 nM, one order of magnitude), thus demonstrating that the nanostructures are capable of improving the analytical performance of the sensor. (C) 2010 Elsevier B.V. All rights reserved.
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| 6. |
- Bossi, Alessandra, et al.
(författare)
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Patterned gallium surfaces as molecular mirrors
- 2007
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Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 23:2, s. 290-294
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Tidskriftsartikel (refereegranskat)abstract
- An entirely new means of printing molecular information on a planar film, involving casting nanoscale impressions of the template protein molecules in molten gallium, is presented here for the first time. The metallic imprints not only replicate the shape and size of the proteins used as template. They also show specific binding for the template species. Such a simple approach to the creation of antibody-like properties in metallic mirrors can lead to applications in separations, microfluidic devices, and the development of new optical and electronic sensors, and will be of interest to chemists, materials scientists, analytical specialists, and electronic engineers.
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| 7. |
- Bossi, Alessandra, et al.
(författare)
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Properties of poly-aminophenylboronate coatings in capillary electrophoresis for the selective separation of diastereoisomers and glycoproteins
- 2004
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Ingår i: Journal of Chromatography A. - : Elsevier. - 0021-9673 .- 1873-3778. ; 1023:2, s. 297-303
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Tidskriftsartikel (refereegranskat)abstract
- The polymerisation of 3-aminophenylboronic acid (APBA) in aqueous environment has been used for the open tubular modification of capillary electrophoresis (CE) capillaries. Being poly-APBA endowed with boronic acid, aromatic rings and secondary amines groups, it posses a variety of functional groups affecting selectivity. Diastereoisomers (e.g. ascorbic and isoascorbic acid) and proteins (e.g. haemoglobins) were successfully separated onto poly-APBA column, by means of a combination of electrophoresis and open tubular electrochromatography. The mechanism of selection was investigated: results indicate an interplay between enhancing or silencing the contribution of the protonable functionahties (amino groups, boronic acid). The properties of APBA polymer coating make it attractive for CE separation and for further application in affinity separations and chip technologies.
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| 8. |
- Guha, Arnab, et al.
(författare)
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Direct detection of small molecules using a nano-molecular imprinted polymer receptor and a quartz crystal resonator driven at a fixed frequency and amplitude
- 2020
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Ingår i: Biosensors & bioelectronics. - : ELSEVIER ADVANCED TECHNOLOGY. - 0956-5663 .- 1873-4235. ; 158
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Tidskriftsartikel (refereegranskat)abstract
- Small molecule detection is of wide interest in clinical and industrial applications. However, its accessibility is still limited as miniaturisation and system integration is challenged in reliability, costs and complexity. Here we combined a 14.3 MHz quartz crystal resonator (QCR), actuated and analysed using a fixed frequency drive (FFD) method, with a nanomolecular imprinted polymer for label-free, realtime detection of N-hexanoyl-L-homoserine lactone (199 Da), a gram-negative bacterial infection biomarker. The lowest concentration detected (1 mu M) without any optimisation was comparable with that of a BIAcore SPR system, an expensive laboratory gold standard, with significant enhancement in sensitivity and specificity beyond the state-of-the-art QCR. The analytical formula-based FFD method can potentially allow a multiplexed "QCR-on-chip" technology, bringing a paradigm shift in speed, accessibility and affordability of small molecule detection.
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| 9. |
- Li, Songjun, et al.
(författare)
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A Zipper-Like On/Off-Switchable Molecularly Imprinted Polymer
- 2011
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Ingår i: Advanced Functional Materials. - : Wiley-VCH Verlag Berlin. - 1616-301X .- 1616-3028. ; 21:17, s. 3344-3349
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Tidskriftsartikel (refereegranskat)abstract
- A zipper-like on/off-switchable molecularly imprinted polymer is reported. This unique imprinted polymer was composed of template-imprinted polymeric networks that incorporate zipper-like interactions between poly(acrylamide) (PAAm) and poly(2-acrylamide-2-methyl propanesulfonic acid) (PAMPS). This polymer showed marginal recognition ability towards the imprint species under low temperature conditions, due to the interpolymer interaction between PAAm and PAMPS, which inhibited access to the imprinted networks. In contrast, at relatively high temperatures (such as 40 degrees C), the polymer demonstrated significant molecular recognition ability towards the imprint species resulting from the dissociation of the interpolymer complexes of PAAm and PAMPS, which enabled access to the imprint networks. Unlike previously reported PNIPAm-based imprinted polymers, which demonstrate alterable molecular recognition simply because of the thermosensitive hydrophilicity/hydrophobicity of PNIPAm, this polymer employed a zipper-like supramolecular architecture between PAAm and PAMPS, thereby enabling switchable molecular recognition.
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| 10. |
- Li, Songjun, et al.
(författare)
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Molecularly Imprinted Polymers for Enzyme-Like Catalysis : principle, design and applications
- 2015
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Ingår i: Molecularly imprinted polymers for enzyme-like catalysis<em> </em>. - : Elsevier. - 9780128013014 - 9780128014448 ; , s. 1-17
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Bokkapitel (refereegranskat)abstract
- Selective catalysis remains a significant challenge owing to the lack of a generic protocol suitable for the preparation of selective catalytic materials. A promising approach is to translate the principle of enzyme catalysis for the design of new catalytic materials. Known as a “key-to-lock” technology, molecular imprinting provides a promising perspective by helping create in a straightforward manner binding sites that possess enzyme-like catalytic ability with but higher stability. In this chapter, we focus on discussing some key issues involved in active molecularly imprinted polymers from catalytic applications. The similarity and difference between preparing conventional molecularly imprinted polymers and catalytic imprinted polymers are highlighted. Other aspects relating to the principle, design, and future outlook of catalytic molecularly imprinted polymers are also discussed.
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