| 1. |
- Acevedo, F., et al.
(författare)
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A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4
- 2011
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Ingår i: Brazilian archives of biology and technology. - 1516-8913 .- 1678-4324. ; 54:6, s. 1175-1186
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Tidskriftsartikel (refereegranskat)abstract
- In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead. The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO 4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50°C) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide.
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| 2. |
- Acevedo, F., et al.
(författare)
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Degradation of polycyclic aromatic hydrocarbons by free and nanoclay-immobilized manganese peroxidase from Anthracophyllum discolor
- 2010
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Ingår i: Chemosphere. - : Elsevier BV. - 0045-6535 .- 1879-1298. ; 80:3, s. 271-278
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Tidskriftsartikel (refereegranskat)abstract
- Manganese peroxidase (MnP) produced by Anthracophyllum discolor, a Chilean white rot fungus, was immobilized on nanoclay obtained from volcanic soil and its ability to degrade polycyclic aromatic hydrocarbons (PAHs) compared with the free enzyme was evaluated. At the same time, nanoclay characterization was performed.Nanoclay characterization by transmission electronic microscopy showed a particle average size smaller than 100nm. The isoelectric points (IEP) of nanoclay and MnP from A. discolor were 7.0 and 3.7, respectively, as determined by micro electrophoresis migration and preparative isoelectric focusing. Results indicated that 75% of the enzyme was immobilized on the nanoclay through physical adsorption. As compared to the free enzyme, immobilized MnP from A. discolor achieved an improved stability to temperature and pH. The activation energy (Ea) value for immobilized MnP (51.9kJmol -1) was higher than that of the free MnP (34.4kJmol -1).The immobilized enzyme was able to degrade pyrene (>86%), anthracene (>65%), alone or in mixture, and to a less extent fluoranthene (<15.2%) and phenanthrene (<8.6%). Compared to free MnP from A. discolor, the enzyme immobilized on nanoclay enhanced the enzymatic transformation of anthracene in soil.Overall results indicate that nanoclay, a carrier of natural origin, is a suitable support material for MnP immobilization. In addition, immobilized MnP shows an increased stability to high temperature, pH and time storage, as well as an enhanced PAHs degradation efficiency in soil. All these characteristics may suggest the possible use of nanoclay-immobilized MnP from A. discolor as a valuable option for in situ bioremediation purposes. © 2010 Elsevier Ltd.
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| 3. |
- Acevedo, F., et al.
(författare)
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Degradation of polycyclic aromatic hydrocarbons by the Chilean white-rot fungus Anthracophyllum discolor
- 2011
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Ingår i: Journal of Hazardous Materials. - : Elsevier BV. - 0304-3894 .- 1873-3336. ; 185:1, s. 212-219
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Tidskriftsartikel (refereegranskat)abstract
- The degradation of three- and four-ring polycyclic aromatic hydrocarbons (PAHs) in Kirk medium by Anthracophyllum discolor, a white-rot fungus isolated from the forest of southern Chile, was evaluated. In addition, the removal efficiency of three-, four- and five-ring PAHs in contaminated soil bioaugmented with A. discolor in the absence and presence of indigenous soil microorganisms was investigated. Production of lignin-degrading enzymes and PAH mineralization in the soil were also determined. A. discolor was able to degrade PAHs in Kirk medium with the highest removal occurring in a PAH mixture, suggesting synergistic effects between PAHs or possible cometabolism. A high removal capability for phenanthrene (62%), anthracene (73%), fluoranthene (54%), pyrene (60%) and benzo(a)pyrene (75%) was observed in autoclaved soil inoculated with A. discolor in the absence of indigenous microorganisms, associated with the production of manganese peroxidase (MnP). The metabolites found in the PAH degradation were anthraquinone, phthalic acid, 4-hydroxy-9-fluorenone, 9-fluorenone and 4,5-dihydropyrene. A. discolor was able to mineralize 9% of the phenanthrene. In non-autoclaved soil, the inoculation with A. discolor did not improve the removal efficiency of PAHs. Suitable conditions must be found to promote a successful fungal bioaugmentation in non-autoclaved soils. © 2010 Elsevier B.V.
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| 4. |
- Andersson, Johan, et al.
(författare)
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Biologisk metanisering av syngas från förgasning och pyrolys - lovande koncept mot implementering
- 2024
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Biological methanation of syngas from pyrolysis and gasification – promising concepts for implementation The need for increased biogas production is significant, and in the EU, there are plans for a substantial expansion in the coming years through the RePowerEU initiative. Part of the increase will come from the expansion of conventional digestion technology, where organic materials such as food waste, manure, and crop residues are used for biogas production. However, to meet the future increased demand, it is also necessary to utilize more difficult-to-digest substrates, such as biomass rich in lignocellulose, for biogas production. This could be forest residues such as branches and tops, sawdust, or bark. This type of substrates cannot be used in a conventional digestion process, and other technology chains are therefore required to convert such biomass into biomethane. This can be done by first converting the biomass into syngas through a thermochemical process such as gasification or pyrolysis. This is followed by a methanation process where the syngas is converted into biogas, and finally, the gas is upgraded to reach biomethane quality. These types of technology chains are not currently available on a commercial scale, but they have been demonstrated, for example, through the Gobigas project, where gasification was followed by catalytic methanation for biomethane production. As full-scale implementation of catalytic methanation of bio-syngas has not yet been achieved, thereis a need to develop alternative conversion technologies that can more cost-effectively achieve the methanation of woody biomass. One possible opportunity for to this is to apply biological methanation instead of a catalytic process. A biological process comes with several advantages, including a greater ability to handle contaminants, higher selectivity in the conversion of syngas, and operation at relatively low temperature and pressure, which simplifies material selection and reactor design. RISE, together with its partners, are developing a concept based on biological methanation of syngas. This project has examined the biological process's ability to handle contaminants in syngas through continuous experiments in carrier-filled trickle bed reactors with an active volume of 5 liters. The process's ability to handle and break down contaminants is an important parameter that can affect and simplify the design of the gas cleaning that occurs after gasification or pyrolysis. Another aspect of the project has been to put the experimental results into context at the concept and system level. Different production techniques for syngas have been mapped out, which could be combined with biological methanation. Based on the mapping, three types of plants have been selected for more detailed analyses of techno-economics, carbon footprint, and opportunities for increased carbon efficiency. The methanation experiments lasted for 552 days, and overall, it was a stable process with high turnover of syngas and high methane production over a long time. There have been some operational disturbances, mainly related to the supply of gas to the process (i.e. delivery of gas cylinders). However, biochemical inhibition or disturbances have been rare, demonstrating a high robustness for biological methanation of syngas. The breakdown of contaminants has been excellent in the process, with levels decreasing below the detection limit. At the same time, as contaminants have been continuously added to the process, microbiology has been able to maintain high turnover of hydrogen and carbon monoxide to methane. The specific methane production was high both during the reference period without contaminants and during the experimental periods with added contaminants. During long periods, the specific methane production has been around 4 L CH4/Lbed volume /day, which is about 4 times higher than our previously achieved results. The transition to thermophilic temperature and using carriers with higher effective surface area has contributed to this increase. During the project, three types of plants have been selected for more detailed analysis: 1) Gasification with Cortus process, which generates a relatively clean syngas with minimal purification needs before biological methanation. There is no need for co-location with a heating plant, but it is an advantage if there is access to the district heating network to sell waste heat. 2) Gasification with Bioshares' concept, where the gasifier is integrated into a larger cogeneration plant and where the produced syngas is purified with an RME-scrubber before biological methanation. Co-location with a larger cogeneration plant provides interesting synergies and integration opportunities, but also sets the boundaries for where the plants can be located. 3) Slow pyrolysis according to Envigas' concept, where the primary product is biochar and where the produced syngas is seen as a by-product. The syngas contains some impurities but generally requires no other purification than cooling to the right temperature (condensing out tars) before being added to biological methanation. This type of plant differs from plant types 1-2 in that the syngas formed is not the primary product, and the syngas has a relatively low energy value compared to the others. Syngas from plant types 2 and 3 contains some hydrocarbons (C1-C3) that are considered inert over the methanation step and therefore do not negatively affect the process. This means that heavier hydrocarbons do not need to be removed upstream, which would likely have been required with catalytic methanation. This leads to a higher system efficiency, and the need for reactor capacity for biological methanation decreases since there is less gas to be processed (more of the end-product consists of hydrocarbons already formed during the thermochemical conversion upstream). For all plant types, downstream of the methanation step, there is a need for further gas purification and upgrading. During the upgrading step carbon dioxide is separated to reach the product specification required by the end user. If long distance distribution is required a final process step consisting of a liquefaction plant for the production of liquid biogas (LBG) can be added to the concept. As another option, the systems can be supplemented with treatment of the carbon dioxide flow out of the upgrading plant, where the flow is processed by drying, compression, and cooling to produce liquid carbon dioxide. For plant type 2, where benzene is present in the syngas, this gas is expected to be separated with relatively high precision in the system and thereby generate a small flow of liquid benzene as a side product. The carbon dioxide emissions for the final product LBG are in the range of 1.6 to 2.6 gCO2-eq/MJLBG, which compares favorably to other types of second-generation biofuels. Compared to fossil gas, the reduction in greenhouse gas emissions is 96-97%. The carbon efficiency of the systems can be significantly increased if excess carbon dioxide is utilized either through BECCS or BECCU. If the carbon dioxide stream from the upgrading plant is processed into liquid carbon dioxide, the production cost is estimated to be 187-204 SEK/ton. If the product is to be sent to permanent storage the cost for transportation and storage would need to be added to estimate total cost of BECCS, but this is out of scope for the current project.. Assuming that BECCS is applied and that the entire carbon sink is allocated to the final product LBG, this will result in negative emissions in the range of -35 to -104 gCO2-eq/MJLBG. An alternative is to utilize excess carbon dioxide directly in the methanation process by boosting incoming gas with extra hydrogen. Hydrogen and carbon dioxide are then converted by methanogens, which generates extra methane. Since the addition of extra hydrogen is assumed to come from electrolysis, the additional methane production can likely be classified as electrofuel, so-called e-methane. The techno-economic evaluation results in a production cost ranging from 740 to 1300 SEK/MWhLBG, including all sensitivity scenarios. The lower price scenarios include a lower investment cost, which can be assumed to represent cases with public investment support. Overall, a large part of the scenarios are considered to be within the range of what can be considered market relevant production costs. This leads to the conclusion that there is techno-economic potential at this stage to justify continued development of concepts based on biological methanation of syngas. With scaling up and continued development in the right direction, the concepts may eventually lead to cost-effective utilization of forest residues for the production of biomethane at a commercially relevant scale. The next step in the development is scaling up to pilot scale, which will take place during 2023-2025 through an EU-funded project and will be carried out by RISE, Wärtsilä, Cortus and Swedish Gas Association. A pilot plant for biological methanation will then be operated with syngas from Cortus' gasifier in Höganäs.
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| 5. |
- Bard, Sara, et al.
(författare)
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Pulp Wastewater Treatment Using Anaerobic Moving Bed Biofilm Reactors: A Case-Study
- 2024
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Ingår i: International Conference on Wider-Uptake of Water Resource Recovery from Wastewater Treatment, ICWRR 2024. - : Springer Nature. ; , s. 234-239
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Konferensbidrag (refereegranskat)abstract
- The pulp and paper (P&P) industry holds significant global importance. However, the industry’s processes substantially demand water and energy resources. Consequently, there is a pressing need for the industry to adopt more sustainable production practices, aiming to trim environmental impact and strengthen resilience against climate change. Recent research has highlighted the potential for substantial increases in Swedish biogas production using anaerobic wastewater (WW) treatment methods within P&P mills. The P&P sector traditionally relies on aerobic biological WW treatment, overlooking the valuable opportunity for WW resource recovery. This study aims to evaluate anaerobic moving bed biofilm reactors (AnMBBR) to enhance energy recovery while enabling treatment capacity for the P&P industry’s anaerobic WW treatment. The results of this study showed the resilience of the AnMBBR system. It proved capable of recovering from overload conditions and operating even during prolonged periods at low pH levels. However, the system removes mainly soluble COD, suggesting higher capabilities on P&P WW with a high fraction of soluble COD. Its ability to produce methane-rich biogas demonstrates efficient gas production while maintaining simple operational procedures.
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| 6. |
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| 7. |
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| 8. |
- Cheng, G., et al.
(författare)
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Microbial community development during syngas methanation in a trickle bed reactor with various nutrient sources
- 2022
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Ingår i: Applied Microbiology and Biotechnology. - : Springer Science and Business Media Deutschland GmbH. - 0175-7598 .- 1432-0614. ; 106, s. 5317-5333
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Tidskriftsartikel (refereegranskat)abstract
- Microbial community development within an anaerobic trickle bed reactor (TBR) during methanation of syngas (56% H2, 30% CO, 14% CO2) was investigated using three different nutrient media: defined nutrient medium (241 days), diluted digestate from a thermophilic co-digestion plant operating with food waste (200 days) and reject water from dewatered digested sewage sludge at a wastewater treatment plant (220 days). Different TBR operating periods showed slightly different performance that was not clearly linked to the nutrient medium, as all proved suitable for the methanation process. During operation, maximum syngas load was 5.33 L per L packed bed volume (pbv) & day and methane (CH4) production was 1.26 L CH4/Lpbv/d. Microbial community analysis with Illumina Miseq targeting 16S rDNA revealed high relative abundance (20–40%) of several potential syngas and acetate consumers within the genera Sporomusa, Spirochaetaceae, Rikenellaceae and Acetobacterium during the process. These were the dominant taxa except in a period with high flow rate of digestate from the food waste plant. The dominant methanogen in all periods was a member of the genus Methanobacterium, while Methanosarcina was also observed in the carrier community. As in reactor effluent, the dominant bacterial genus in the carrier was Sporomusa. These results show that syngas methanation in TBR can proceed well with different nutrient sources, including undefined medium of different origins. Moreover, the dominant syngas community remained the same over time even when non-sterilised digestates were used as nutrient medium. Key points: •Independent of nutrient source, syngas methanation above 1 L/Lpbv/D was achieved. •Methanobacterium and Sporomusa were dominant genera throughout the process. •Acetate conversion proceeded via both methanogenesis and syntrophic acetate oxidation. Graphical abstract: [Figure not available: see fulltext.] © 2022, The Author(s).
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| 9. |
- Englund, Maja, 1982-, et al.
(författare)
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Läkemedel i källsorterade avloppsfraktioner - en kunskapssammanställning
- 2019
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Källsorterande avloppssystem kan minska utsläpp av läkemedelsrester till akvatiska miljöer och möjliggör samtidigt kretslopp av näringsämnen. Kunskapen om läkemedels-förekomst i källsorterade avloppsfraktioner är dock delvis bristfällig, liksom kunskapen om vilken reducerande effekt som erhålls i de behandlings- och hanteringsprocesser som används för dessa fraktioner idag. Även kunskapen om vad som händer i miljön är begränsad vad gäller upptag i växter, nedbrytning, transport och spridning.Syftet med projektet var att samla den kunskap som finns och den forskning som pågår relaterad till läkemedel i källsorterade avloppsfraktioner i Sverige och internationellt, för att identifiera prioriterade frågeställningar framåt. Projektet syftade även till att beskriva hur behandling av avloppsfraktioner från källsorterande avloppssystem påverkar halterna av läkemedelsrester i slutprodukten.Projektet genomfördes utifrån en litteraturstudie med fokus på genomförda och pågående studier/forskning relaterad till läkemedel i källsorterade avloppsfraktioner globalt. Inga analyser har genomförts inom projektet. De data över innehåll av läkemedelssubstanser i obehandlade och behandlade fraktioner som redovisas är hämtade från tidigare genomförda studier. Förutsättningarna för studier kring läkemedel i källsorterade avloppsfraktioner varierar, vilket försvårar möjligheten att jämföra resultat och dra slutsatser kring innehåll och reduktion av läkemedel i källsorterade avloppsfraktioner.De flesta studier behandlar urin. Merparten av behandlingsmetoderna för urin är utförda i labbskala medan studier på klosettavloppsvatten är gjorda på anläggningar som är i drift idag. För latrin har endast en studie som behandlar läkemedel hittats.Olika behandlingsmetoder fungerar olika bra på olika typer av läkemedelssubstanser. För urin har tester utförts med många olika behandlingstekniker. Av de som beaktats i denna studie är det endast ozon och UV-ljus som har en bred effekt och som till störst del reducerar de flesta läkemedelssubstanser som har analyserats i urin. För klosettavloppsvatten har tre behandlingsmetoder studerats. Ingen av metoderna reducerade alla läkemedel, men behandling med UASB-reaktor gav en god reduktion då de flesta läkemedel som analyserats reducerades till ca 60 %. För latrin påverkades de flesta läkemedel varken av mesofil eller termofil rötning.De flesta studierna kring läkemedelssubstanser i miljön har fokus på akvatiska system och informationen om hur substanserna beter sig i marken är begränsade – både vad gäller nedbrytning samt läkemedelsinnehåll i växande gröda. Inom dessa områden behövs mer forskning.Läkemedel i källsorterade avloppsfraktioner är ett komplext område där flera kunskapsluckor finns och där mer forskning behövs. Förhoppningsvis bidrar denna sammanställning till en översiktlig bild av hur det kan se ut, vilken kunskap som finns inom området samt förenklar beslut och prioritering av framtida forskning.
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| 10. |
- Gao, Wenxuan, et al.
(författare)
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Evaluation of spent mushroom substrate as substitute of peat in Chinese biobeds
- 2015
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Ingår i: International Biodeterioration & Biodegradation. - : Elsevier BV. - 0964-8305 .- 1879-0208. ; 98, s. 107-112
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Tidskriftsartikel (refereegranskat)abstract
- Biobeds are an effective system for minimising the risk of point-source contamination by pesticides. The original biobed system comprises a clay layer at the bottom, a biomixture layer and a grass layer on top. The composition of the biomixture, which in the original Swedish model consisted of soil: peat: wheat straw (1:1:2 by volume), determines the efficiency of the system. Since the use of peat is not environmentally or economically feasible in China, this study tested the potential of replacing it with a locally available material, spent mushroom substrate (SMS). Three biomixtures containing different SMS (Pleurotus eryngii, Flammulina velutipes and Lentinus edodes) were compared with a biomixture containing peat, as in the original Swedish design, and a control containing soil alone. The fungicide chlorothalonil and the insecticide imidacloprid were used as model pesticides in the tests. Microbial activity (measured as respiration and phenoloxidase and hydrolytic activity) and pesticide dissipation were studied. Microbial activity was higher in the three biomixtures containing SMS than in the original-type biomixture. Among the SMS biomixtures, that containing SMS from L.edodes was the most biologically active. However, pesticide dissipation was comparable in all four biomixtures and significant differences were only found between biomixtures and the soil-alone control. Based on the physicochemical characteristics, biological activity and preliminary results on pesticide degradation, SMS are suitable and can therefore be used as a substitutes for peat.
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