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- Kistler, J. O., et al.
(författare)
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The oral microbiome in human immunodeficiency virus (HIV)-positive individuals
- 2015
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Ingår i: Journal of Medical Microbiology. - : Microbiology Society. - 0022-2615 .- 1473-5644. ; 64:Part: 9, s. 1094-1101
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Tidskriftsartikel (refereegranskat)abstract
- Human immunodeficiency virus (HIV) infection is associated with a range of oral conditions, and increased numbers of disease-associated microbial species have previously been found in HIV-positive subjects. The aim of this study was to use next-generation sequencing to compare the composition of the oral microbiome in HIV-positive and -negative individuals. Plaque and saliva were collected from 37 HIV-positive individuals and 37 HIV-negative individuals, and their bacterial composition determined by pyrosequencing of partial 16S rRNA genes. A total of 855 222 sequences were analysed. The number of species-level operational taxonomic units (OTUs) detected was significantly lower in the saliva of HIV-positive individuals (mean=303.3) than in that of HIV-negative individuals (mean=365.5) (P<0.0003). Principal coordinates analysis (PCoA) based on community membership (Jaccard index) and structure (Yue and Clayton measure of dissimilarity) showed significant separation of plaque and saliva samples [analysis of molecular variance (AMOVA), P<0.001]. PCoA plots did not show any clear separation based on HIV status. However, AMOVA indicated that there was a significant difference in the community membership of saliva between HIV-positive and -negative groups (P=0.001). Linear discriminant analysis effect size revealed an OTU identified as Haemophilus parainfluenzae to be significantly associated with HIV-positive individuals, whilst Streptococcus mitis/HOT473 was most significantly associated with HIV-negative individuals. In conclusion, this study has confirmed that the microbial composition of saliva and plaque is different. The oral microbiomes of HIV-positive and -negative individuals were found to be similar overall, although there were minor but significant differences in the composition of the salivary microbiota of the two groups.
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- Payungporn, S., et al.
(författare)
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Identification of Bacteria Associated with a Periodontal Disease in Thai Patients Based on Next-Generation Sequencing
- 2017
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Ingår i: Jundishapur Journal of Microbiology. - : Briefland. - 2008-3645 .- 2008-4161. ; 10:6
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Tidskriftsartikel (refereegranskat)abstract
- Background: Oral diseases depend on the relationship between host and various species of a bacterial community. The propagation of pathogenic bacteria within the mouth can cause periodontitis. Objectives: In this study, bacterial classifications were performed based on next-generation sequencing (NGS) of 16S ribosomal DNA (rDNA) in order to identify potential bacterial species associated with a periodontal disease in Thai patients. Methods: Dental plaques were collected from healthy controls (n = 5; mean age = 48.4 +/- 4.5 years) and patients with a chronic periodontitis (n = 5; mean age = 47.4 +/- 10.1 years). Total DNA was extracted and then amplified by specific primers within a V3/ V4 region of the 16S rDNA gene. The purified DNA from samples within the same group were pooled together and used to construct DNA libraries with different indexes. High- throughput sequencing with paired- end (250 x 2) was carried out on a MiSeq platform. Pass- filter sequencing reads (Q >= 30) were used for bacterial classification. Results: The comparative analysis of healthy controls and patients with a chronic periodontitis revealed that Porphyromonas gingivalis and Prevotella intermedia were significantly associated with a periodontal disease. Other bacteria such as Treponema denticola, T. medium, Tannerella forsythia, P. endodontalis and Filifactor alocis might be potentially associated with the periodontal disease in Thai patients. Conclusions: Several potential bacteria that might be associated with periodontal disease in Thai patients were identified. The obtained data from this study would be useful for understanding the bacterial communities which is responsible for periodontal disease that might be applied for more specific bacteria- targeted antimicrobial therapy of the disease.
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