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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2. |
- Ma, Liyao, et al.
(författare)
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Apple grading method based on neural network with ordered partitions and evidential ensemble learning
- 2022
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Ingår i: CAAI Transactions on Intelligence Technology. - : John Wiley & Sons. - 2468-6557 .- 2468-2322. ; 7:4, s. 561-569
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Tidskriftsartikel (refereegranskat)abstract
- In order to improve the performance of the automatic apple grading and sorting system, in this paper, an ensemble model of ordinal classification based on neural network with ordered partitions and Dempster–Shafer theory is proposed. As a non-destructive grading method, apples are graded into three grades based on the Soluble Solids Content value, with features extracted from the preprocessed near-infrared spectrum of apple serving as model inputs. Considering the uncertainty in grading labels, mass generation approach and evidential encoding scheme for ordinal label are proposed, with uncertainty handled within the framework of Dempster–Shafer theory. Constructing neural network with ordered partitions as the base learner, the learning procedure of the Bagging-based ensemble model is detailed. Experiments on Yantai Red Fuji apples demonstrate the satisfactory grading performances of proposed evidential ensemble model for ordinal classification. © 2022 The Authors. CAAI Transactions on Intelligence Technology published by John Wiley & Sons Ltd on behalf of The Institution of Engineering and Technology and Chongqing University of Technology.
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