| 1. |
- Bostanci, Nagihan, et al.
(författare)
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Label-free quantitative proteomics reveals differentially regulated proteins in experimental gingivitis
- 2013
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 2:12, s. 657-678
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Tidskriftsartikel (refereegranskat)abstract
- We investigated the sequential protein expression in gingival crevicular fluid samples during the induction (I) and resolution (R) of experimental gingivitis. Periodontally and systemically healthy volunteers (n = 20) participated in a three-week experimental gingivitis protocol, followed by debridement and two weeks of regular plaque control. Gingival crevicular fluid (GCF) samples were collected at baseline, Day 7, 14, and 21 (induction; I-phase), and at Day 21, 25, 30, and 35 (resolution; R-phase). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for label-free quantitative proteomics was applied. A total of 287 proteins were identified including 254 human, 14 bacterial, 12 fungal, and 7 yeast proteins. Ontology analysis revealed proteins primarily involved in cytoskeletal rearrangements, immune response, antimicrobial function, protein degradation, and DNA binding. There was considerable variation in the number of proteins identified, both among subjects and within subjects across time points. After pooling of samples between subjects at each time point, the levels of 59 proteins in the I-phase and 73 proteins in the R-phase were quantified longitudinally. Our data demonstrate that LC-MS/MS label-free quantitative proteomics is valuable in the assessment of the protein content of the GCF and can facilitate a better understanding of the molecular mechanisms involved in the induction and resolution of plaque-induced gingival inflammation in humans.
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| 2. |
- Charalampakis, Georgios, et al.
(författare)
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Effect of cleansing of biofilm formed on titanium discs.
- 2015
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Ingår i: Clinical oral implants research. - : Wiley. - 1600-0501 .- 0905-7161. ; 26:8, s. 931-936
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Tidskriftsartikel (refereegranskat)abstract
- To study the combined effect of mechanical and chemical cleansing on a 4-day biofilm grown intra-orally on titanium discs with different surface characteristics.
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| 3. |
- Fadel, Hani T, 1979, et al.
(författare)
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Clinical and biological indicators of dental caries and periodontal disease in adolescents with or without obesity.
- 2014
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Ingår i: Clinical oral investigations. - : Springer Science and Business Media LLC. - 1436-3771 .- 1432-6981. ; 18:2, s. 359-368
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: This study aims to assess clinical, microbiological and inflammatory parameters as indicators for caries and periodontal disease in adolescents with obesity. MATERIAL AND METHODS: Twenty-seven adolescents with obesity [body mass index (BMI) 37±4kg/m(2)] and 28 controls (BMI 20±2kg/m(2)) answered questionnaires and were investigated regarding salivary parameters, plaque pH drop after a 1-min glucose rinse, oral clinical parameters, inflammatory markers in gingival crevicular fluid (GCF) and sub-gingival mirobiota. RESULTS: Compared with controls, adolescents with obesity had a lower stimulated salivary secretion rate (1.55±0.63 vs. 2.05±1.05mL/min, p<0.05), higher concentrations of secretory immunoglobulin A (sIgA) (p<0.001), more decayed tooth surfaces (3.4±6.6 vs. 0.8±1.1, p<0.05) and more gingivitis (p<0.01) after controlling for possible confounders. Overall, similar snacking habits, plaque amounts and numbers of deep periodontal pockets were observed. Following the glucose rinse, a slightly more pronounced drop in plaque pH was observed in the obesity group (p>0.05). No differences in sub-gingival inflammatory or microbial indicators were detected (p>0.01). CONCLUSIONS: More caries and gingival inflammation were observed in adolescents with obesity. Of the indicators tested, salivary secretion rate was lower and sIgA levels were higher in the obesity group. We are unable to confirm whether differences in caries and gingival inflammation are due to systemic changes that are associated with obesity or due to possible irregular dietary/oral hygiene habits. CLINICAL RELEVANCE: Customised oral health preventive programmes and appropriate collaboration with medical personnel in selecting the best diet, medication and psychological support can help improve the general well-being, including oral health, of children with obesity. This may even reduce the risk of oral diseases.
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| 4. |
- Hellström, Maj-Karin, 1948, et al.
(författare)
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Local minocycline as an adjunct to surgical therapy in moderate to severe, chronic periodontitis.
- 2008
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Ingår i: Journal of clinical periodontology. - 1600-051X. ; 35:6, s. 525-531
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Tidskriftsartikel (refereegranskat)abstract
- Objective: The aim of the study was to evaluate the effects of minocycline microspheres on periodontal probing depth reduction when used in combination with surgery in adults with moderate to severe, chronic periodontitis. Material and Methods: Sixty patients with a minimum of one non-molar periodontal site >/=6 mm in two oral quadrants received either local minocycline microspheres at baseline, immediately following each of two surgical therapies (Weeks 2 and 3), and at Week 5 or surgery alone. Results: The mean probing depth reduction at Week 25 at sites >/=5 mm at baseline was 2.51 mm in the test group and 2.18 mm in the control group. Smokers in the test group had a significantly greater probing depth reduction (2.30 mm) than smokers in the control group (2.05 mm). The number of sites with probing depth reductions of >/=2 and >/=3 mm were significantly higher in the test group than in the control group. Conclusion: Applications of local minocycline as an adjunct to surgery in adults with moderate to severe, chronic periodontitis were associated with statistically significant greater reductions in probing depth than surgery alone.
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| 5. |
- Hellström, Maj-Karin, 1948, et al.
(författare)
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The effect of a dentifrice containing Magnolia extract on established plaque and gingivitis in man: a six-month clinical study
- 2014
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Ingår i: International Journal of Dental Hygiene. - : Wiley. - 1601-5029. ; 12:2, s. 96-102
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Tidskriftsartikel (refereegranskat)abstract
- Aim The aim was to evaluate the clinical effect of a dentifrice containing 0.3% Magnolia extract on dental plaque and gingivitis. The trial was a 6-month double-blind, stratified, randomized and 2-armed parallel group study. Forty-six subjects in the test group brushed their teeth with a dentifrice containing 0.3% Magnolia extract and 48 subjects in the control group brushed with a placebo dentifrice. Plaque and gingivitis were assessed at baseline, 3 and 6months. There was a significantly larger gingivitis reduction in the Magnolia group than in the control group (0.26 +/- 0.11 versus 0.11 +/- 0.12) (P<0.001). There was a greater increase in the total number of healthy gingival units Gingival Index (GI score 0) in the Magnolia group than in the control group (149% versus 31%) and a larger reduction in inflamed gingival units (GI score 2/3) (60% versus 30%). Furthermore, at sites with similar amounts of plaque, less clinical signs of gingival inflammation were observed in the Magnolia group than in the control group. Six months' unsupervised use of a dentifrice containing 0.3% Magnolia extract resulted in significantly greater gingivitis reduction than a corresponding control dentifrice.
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| 6. |
- Jönsson, Daniel, et al.
(författare)
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Gingival tissue transcriptomes in experimental gingivitis.
- 2011
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Ingår i: Journal of clinical periodontology. - : John Wiley & Sons. - 1600-051X .- 0303-6979. ; 38:7, s. 599-611
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Tidskriftsartikel (refereegranskat)abstract
- We investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis.
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| 7. |
- Konradsson, Katarina, et al.
(författare)
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Stabilized stannous fluoride dentifrice in relation to dental caries, dental erosion and dentin hypersensitivity: A systematic review
- 2020
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Ingår i: American Journal of Dentistry. - : Mosher & Linder. - 0894-8275. ; 33:2, s. 95-105
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: To review the scientific evidence for the efficacy of stabilized stannous fluoride (SnF2) dentifrice in relation to dental caries, dental erosion and dentin hypersensitivity. Methods: Medline OVID, Embase.com, and the Cochrane Library were searched until January 2018. Two researchers independently selected studies according to inclusion and exclusion criteria, data were extracted, the risk of bias in eligible studies was assessed and a meta-analysis was performed wherever feasible. Results: Three studies on dental caries, eight studies on dental erosion and 11 on dentin hypersensitivity were included. Risk of bias was judged as high for most of the caries and erosion studies and low or medium for the hypersensitivity studies. A similar or slightly higher anti-caries activity compared with nonstannous fluoride dentifrices was observed. Stannous fluoride showed a greater anti-erosive potential in seven of the eight studies. A mean difference score in favor of stannous fluoride dentifrices compared with controls was found in a meta-analysis including six 8-week hypersensitivity studies.
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| 8. |
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| 9. |
- Montén, Ulrika, et al.
(författare)
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Periodontal conditions in male adolescents using smokeless tobacco (moist snuff).
- 2006
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Ingår i: Journal of clinical periodontology. - 0303-6979. ; 33:12, s. 863-8
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Tidskriftsartikel (refereegranskat)abstract
- AIM: The aim of this study was to evaluate the potential association of the use of smokeless tobacco (moist snuff) on the periodontal conditions of adolescents. MATERIAL AND METHODS: A subject sample of one hundred and three 19-year-old male individuals (33 snuff users, 70 controls) living in G?teborg, Sweden, were clinically examined with regard to oral hygiene, gingivitis, probing pocket depth (PPD), clinical attachment loss (CAL) and gingival recession. Bitewing radiographs were obtained for assessments of alveolar bone level. Information about tobacco and oral hygiene habits was obtained by a structured questionnaire. Student 's t-test, chi(2)-test and logistic regression analysis were used for statistical analysis. RESULTS: The mean plaque and gingivitis scores in snuff-users were 59% (SD 21.0) and 47% (18.6), respectively, and in controls 64% (22.4) and 50% (18.3), respectively. The average PPD and CAL in snuff-users amounted to 2.3 mm (0.3) and 0.2 mm (0.1), respectively, and in controls 2.4 mm (0.3) and 0.1 mm (0.1) (p>0.05), respectively. The mean bone level was 1.3 mm (0.2) in both groups. The prevalence of subjects showing recession was 42% among snuff-users and 17% among controls (p=0.006). In snuff users, an average of 4% (0.9) of the teeth showed recession, compared with 1% (0.3) in controls (p<0.001). Limiting the analysis to the maxillary anterior tooth region, 33% of the snuff-users and 10% of the controls presented recessions (p=0.002). The use of snuff entailed an OR=5.1 to have gingival recessions. CONCLUSION: In the present population sample of adolescents, the use of smokeless tobacco (moist snuff) was not associated with the presence of periodontal disease except for a significantly high prevalence of gingival recessions.
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| 10. |
- Mystikos, Chrysostomos, et al.
(författare)
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Effect of post-brushing mouthrinse solutions on salivary fluoride retention.
- 2011
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Ingår i: Swedish dental journal. - 0347-9994. ; 35:1, s. 17-24
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Tidskriftsartikel (refereegranskat)abstract
- Fluoride (F) toothpaste is one of the most effective means of caries prevention. There is also evidence that mouthrinse solutions with antimicrobial agents reduce plaque formation and gingivitis and may be used as adjuncts to daily self-performed oral hygiene for risk patients. The authors hypothesize that using these solutions--without or with just a low F concentration after brushing--will have a "wash-out" effect on F toothpaste. Mouthrinse solutions with more F might be beneficial in this respect. Two groups of 10 (Series 1) and 12 (Series II) healthy subjects were recruited. They brushed for 1 min with toothpastes containing either 1450 or 5000 ppm F. After brushing and spitting out the toothpaste, the participants in Series I rinsed for 30 sec with 10 ml of a variety of products with various F concentrations (0, 100, 226 or 900 ppm F). In Series II, they first rinsed with water after the brushing and directly thereafter with 20 ml of the post-brushing rinsing solution for 30 sec. Saliva samples in both series were collected at different time points up to 1 h and the F concentration was measured. There was significantly less F in saliva after rinsing with no F or with a low F concentration (100 ppm) compared with just brushing with a F toothpaste. Rinsing with 226 ppm F displayed significantly higher F concentrations in saliva compared with only toothbrushing. Products with a high F concentration (i.e. toothpaste with 5000 ppm F or a mouthrinse solution with 900 ppm F) produced the highest F retention in saliva compared with all other protocols. The quantity of mouthrinse solution (20 vs. 10 ml) did not seem to have any effect on the F retention. The results from both test series show that a post-brushing rinsing solution without F or with just 100 ppm F exerts a "wash-out" effect on toothbrushing with either 1450 or 5000 ppm F, which may be negative for all patients, especially those with a risk of caries. The general population will benefit more from higher concentrations of F in mouthrinse solutions and, based on the results of the present investigation, 226 ppm F (corresponding to 0.05% NaF) should be the lowest concentration used. Furthermore, caries risk patients are recommended to use a high-F toothpaste (5000 ppm F) or a post-brushing mouthrinse solution with 900 ppm F (corresponding to 0.2% NaF).
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