1. |
- Lombard, Vincent, et al.
(författare)
-
A hierarchical classification of polysaccharide lyases for glycogenomics
- 2010
-
Ingår i: Biochemical Journal. - 0264-6021 .- 1470-8728. ; 432, s. 437-444
-
Tidskriftsartikel (refereegranskat)abstract
- Carbohydrate-active enzymes face huge substrate diversity in a highly selective manner using only a limited number of available folds. They are therefore subjected to multiple divergent and convergent evolutionary events. This and their frequent modularity render their functional annotation in genomes difficult in a number of cases. In the present paper, a classification of polysaccharide lyases (the enzymes that cleave polysaccharides using an elimination instead of a hydrolytic mechanism) is shown thoroughly for the first time. Based on the analysis of a large panel of experimentally characterized polysaccharide lyases, we examined the correlation of various enzyme properties with the three levels of the classification: fold, family and subfamily. The resulting hierarchical classification, which should help annotate relevant genes in genomic efforts, is available and constantly updated at the Carbohydrate-Active Enzymes Database (http://www.cazy.org).
|
|