| 1. |
- Gopal, Dhivyasri, et al.
(författare)
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Optimization of processing parameters of cold metal transfer joined 316L and weld bead profile influenced by temperature distribution based on genetic algorithm
- 2022
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Ingår i: Proceedings of the Institution of mechanical engineers. Part C, journal of mechanical engineering science. - : Sage Publications. - 0954-4062 .- 2041-2983. ; 236:19
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Tidskriftsartikel (refereegranskat)abstract
- Austenitic stainless steel alloys find the wide range of application in modern industries like pipework, containers, food production and in medical industries for its excellent processing properties and corrosion resistance. There is enormous literature report on the mechanical properties, appropriate joining of materials using different fusion welding processes. Consequently, the cold metal transfer technique appears to weld materials with low heat input which is a noticeable feature of this welding process. In this paper, cold metal transfer welding is performed on austenitic stainless steel material 316L and its bead geometries such as reinforcement height, depth of weld penetration and bead width profile are examined. The temperature distribution at the welding line is observed by means of the data acquisition unit. Genetic algorithm based optimization technique is used to achieve the desired combination of input variables and weld bead geometry. This developed genetic algorithm optimizes the welding process parameters and geometry of the weld bead, by minimizing the least square error based objective function. The investigation outcome of this paper provides an insight into the characterization of the weldment, the effects of weld current and weld travel speed on temperature profile and mechanical properties include hardness, tensile and residual profiles.
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| 2. |
- Reddy, B. K. Kishore, et al.
(författare)
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Assessment of Mycobacterium tuberculosis Pantothenate Kinase Vulnerability through Target Knockdown and Mechanistically Diverse Inhibitors
- 2014
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Ingår i: Antimicrobial Agents and Chemotherapy. - 0066-4804 .- 1098-6596. ; 58:6, s. 3312-3326
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Tidskriftsartikel (refereegranskat)abstract
- Pantothenate kinase (PanK) catalyzes the phosphorylation of pantothenate, the first committed and rate-limiting step toward coenzyme A (CoA) biosynthesis. In our earlier reports, we had established that the type I isoform encoded by the coaA gene is an essential pantothenate kinase in Mycobacterium tuberculosis, and this vital information was then exploited to screen large libraries for identification of mechanistically different classes of PanK inhibitors. The present report summarizes the synthesis and expansion efforts to understand the structure-activity relationships leading to the optimization of enzyme inhibition along with antimycobacterial activity. Additionally, we report the progression of two distinct classes of inhibitors, the triazoles, which are ATP competitors, and the biaryl acetic acids, with a mixed mode of inhibition. Cocrystallization studies provided evidence of these inhibitors binding to the enzyme. This was further substantiated with the biaryl acids having MIC against the wild-type M. tuberculosis strain and the subsequent establishment of a target link with an upshift in MIC in a strain overexpressing PanK. On the other hand, the ATP competitors had cellular activity only in a M. tuberculosis knockdown strain with reduced PanK expression levels. Additionally, in vitro and in vivo survival kinetic studies performed with a M. tuberculosis PanK (MtPanK) knockdown strain indicated that the target levels have to be significantly reduced to bring in growth inhibition. The dual approaches employed here thus established the poor vulnerability of PanK in M. tuberculosis.
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