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- Hober, Andreas, et al.
(författare)
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Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis
- 2021
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Ingår i: eLIFE. - : eLIFE SCIENCES PUBL LTD. - 2050-084X. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Reliable, robust, large-scale molecular testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for monitoring the ongoing coronavirus disease 2019 (COVID-19) pandemic. We have developed a scalable analytical approach to detect viral proteins based on peptide immuno-affinity enrichment combined with liquid chromatography-mass spectrometry (LC-MS). This is a multiplexed strategy, based on targeted proteomics analysis and read-out by LC-MS, capable of precisely quantifying and confirming the presence of SARS-CoV-2 in phosphate-buffered saline (PBS) swab media from combined throat/nasopharynx/saliva samples. The results reveal that the levels of SARS-CoV-2 measured by LC-MS correlate well with their correspondingreal-time polymerase chain reaction (RT-PCR) read-out (r = 0.79). The analytical workflow shows similar turnaround times as regular RT-PCR instrumentation with a quantitative read-out of viral proteins corresponding to cycle thresholds (Ct) equivalents ranging from 21 to 34. Using RT-PCR as a reference, we demonstrate that the LC-MS-based method has 100% negative percent agreement (estimated specificity) and 95% positive percent agreement (estimated sensitivity) when analyzing clinical samples collected from asymptomatic individuals with a Ct within the limit of detection of the mass spectrometer (Ct <= 30). These results suggest that a scalable analytical method based on LC-MS has a place in future pandemic preparedness centers to complement current virus detection technologies.
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| 2. |
- Namedanian, Mahziar, 1981-, et al.
(författare)
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Analysis of a Three Part 230 kV Optical Voltage Transducer with Multiple Electric Field Sensors
- 2008
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Ingår i: Proceedings from The 8. IASTED European conference on power and energy systems (EuroPES 2008). - Calgary : ACTA Press.
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Konferensbidrag (refereegranskat)abstract
- Optical Voltage Transducer (OVT) is considered as a proper candidate for replacing the conventional inductive and capacitive transformers. The exact positioning of the electric field sensors are one of the important issues while designing an OVT. The three sensors of the OVT have been positioned according to quadrature method to satisfy the IEC 0.2% class specification. A three part OVT is designed and numerically analyzed by FLUX software and the results for different external perturbations are maintaining the 0.2% class accuracies for the given system. The advantages of using a three part OVT have been considered in this paper.
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| 3. |
- Zuo, Zhaoyang, et al.
(författare)
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Effect of magnetic field on tribological properties of IN718 superalloy coating produced by laser cladding on GTD-111 superalloy
- 2022
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Ingår i: Vacuum. - : Elsevier. - 0042-207X .- 1879-2715. ; 203
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to investigate the effect of a magnetic field on the wear behavior of an IN718 layer applied by laser cladding on GTD-111 superalloy. The results showed that by increasing the intensity of the magnetic induction field from 25 mT to 75 mT, the grains become finer and the area of the equiaxed grains increases. This increased the hardness and decreased the porosity of the cladding zone (CZ). However, the greatest magnetostrictive effect was produced at a magnetic intensity of 25 mT, which reduced the elastic modulus of the specimen. Under such conditions, the hardness ratio to the elastic modulus reached its maximum value (3.97), which resulted in increased wear resistance of the CZ. © 2022 Elsevier Ltd
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