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- Barbera, Stefano, et al.
(författare)
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The small GTPase Rab5c is a key regulator of trafficking of the CD93/Multimerin-2/1 integrin complex in endothelial cell adhesion and migration
- 2019
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Ingår i: Cell Communication and Signaling. - : Springer Science and Business Media LLC. - 1478-811X. ; 17
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundIn the endothelium, the single-pass membrane protein CD93, through its interaction with the extracellular matrix protein Multimerin-2, activates signaling pathways that are critical for vascular development and angiogenesis. Trafficking of adhesion molecules through endosomal compartments modulates their signaling output. However, the mechanistic basis coordinating CD93 recycling and its implications for endothelial cell (EC) function remain elusive.MethodsHuman umbilical vein ECs (HUVECs) and human dermal blood ECs (HDBEC) were used in this study. Fluorescence confocal microscopy was employed to follow CD93 retrieval, recycling, and protein colocalization in spreading cells. To better define CD93 trafficking, drug treatments and transfected chimeric wild type and mutant CD93 proteins were used. The scratch assay was used to evaluate cell migration. Gene silencing strategies, flow citometry, and quantification of migratory capability were used to determine the role of Rab5c during CD93 recycling to the cell surface.ResultsHere, we identify the recycling pathway of CD93 following EC adhesion and migration. We show that the cytoplasmic domain of CD93, by its interaction with Moesin and F-actin, is instrumental for CD93 retrieval in adhering and migrating cells and that aberrant endosomal trafficking of CD93 prevents its localization at the leading edge of migration. Moreover, the small GTPase Rab5c turns out to be a key component of the molecular machinery that is able to drive CD93 recycling to the EC surface. Finally, in the Rab5c endosomal compartment CD93 forms a complex with Multimerin-2 and active 1 integrin, which is recycled back to the basolaterally-polarized cell surface by clathrin-independent endocytosis.ConclusionsOur findings, focusing on the pro-angiogenic receptor CD93, unveil the mechanisms of its polarized trafficking during EC adhesion and migration, opening novel therapeutic opportunities for angiogenic diseases.
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| 3. |
- Karlin, Beth, et al.
(författare)
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Process matters : Assessing the use of behavioural science methods in applied behavioural programmes
- 2022
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Ingår i: ECEEE 2022 Summer Study on Energy Efficiency : Agents of Change - Agents of Change. - 1653-7025 .- 2001-7960. - 9789198827002 ; , s. 541-549
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Konferensbidrag (refereegranskat)abstract
- Behavioural science methods have significant potential to help policy makers, practitioners and energy programme managers design, implement and evaluate behavioural campaigns addressing hard-to-reach (HTR) energy users. But when facing budget and logistic constraints, how many real-life programmes actually follow through? How are behavioural science methods actually applied in a real-world setting? This paper presents a scientific process for behaviour change programmes - the “Building Blocks of Behaviour Change” - and analyses 19 case studies from seven countries to see how many are actually utilising these methods in practice. The case studies focus specifically on HTR energy users and the authors also share their perspectives in feasibility and acceptability of utilising such a process in their work. We found that most programmes utilised some behavioural science research methods, but few followed a full scientific “best practice” process. Limitations of this study include selection bias (the case studies for analysis were chosen by country experts), design issues and some missing data in the cases with regards to exact methods employed. But it is the first study of its kind, to our knowledge, that takes a look at the extent of how scientific methods are being applied in the real world with HTR energy users. Based on this comparative analysis of the cases and feedback from the case study authors, we present recommendations on how programmes can continue to realistically integrate best practice methods into their programmes while also meeting budget, competency, and timeline constraints.
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| 4. |
- Mundaca, Luis, et al.
(författare)
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Hard-to-reach energy users : An ex-post cross-country assessment of behavioural-oriented interventions
- 2023
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Ingår i: Energy Research and Social Science. - 2214-6296. ; 104
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Tidskriftsartikel (refereegranskat)abstract
- Hard-to-reach (HTR) energy users encompass individuals who are physically difficult to reach, underserved, or challenging to engage and motivate in demand-side energy programmes. Given a mix of societal challenges (e.g. inequity, energy poverty, decarbonisation, the COVID-19 pandemic), HTR energy users are receiving increasing attention. However, there is a lack of knowledge on the performance of interventions that target (explicitly or implicitly) HTR energy users, particularly from a behaviour change perspective. Our study addresses this knowledge gap, and aims to provide a systematic ex-post comparative cross-country assessment of nineteen case studies, implemented in eight countries. From a methodological point of view, our study explores and tests the usefulness of applying the ‘Building Blocks of Behaviour Change’ (BBBC) in assessing the extent to which interventions employ design and implementation practices that are known to drive behaviour change. Our findings reveal that interventions perform well with respect to the Audience, Behaviour, and Delivery building blocks, but show room for improvement in the Content and Evaluate blocks. Assessing the BBBC framework reveals promising results in terms of credibility, confirmability, transferability, and reliability; however, limitations and uncertainties are also present. Considering the exploratory methodological nature of our study, the results highlight numerous context-specific factors that frame our findings and the suitability of the research approach. We underscore that greater attention must be paid to both the integration of behavioural science methods into HTR interventions, and the systematic analysis of heterogeneity in future HTR-related energy research.
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| 5. |
- Thollander, Patrik, et al.
(författare)
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A review of energy efficiency policies for small and medium-sized manufacturing enterprises from around the world
- 2019
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Ingår i: 2019 ACEEE Summer Study on Energy Efficiency in Industry, Portland, August 12-14, 2019. ; , s. 3-135-3-150
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Konferensbidrag (refereegranskat)abstract
- In most countries, small and medium-sized manufacturing (industrial) enterprises (SMEs) represent more than 99% of the number of companies and 60% of employment. Thus, this sector, apart from using energy, is a major driver in the economy with regard to innovations, GDP, investments and export. Despite the importance of SMEs in the economy, they have not received much attention in most countries' energy policy activities. Energy management in its various forms is regarded as one of the key drivers of industrial energy efficiency. While the term "energy management" is often associated with the ISO 50001 standard, there is a broad variety of different programs and schemes in place that do not strictly abide by the framework of the standard. Especially for SMEs, the standardized protocols of ISO 50001 are often too complex for cost-efficient implementation. The aim of this paper is to provide an international overview of existing energy efficiency policies with a focus on energy management practices in selected EU member states as well as Norway and Japan. Results indicate that different countries apply a broad variety of policy contexts in which the energy management practices are embedded.
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