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- Georgoudaki, A. M., et al.
(författare)
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CD244 is expressed on dendritic cells and regulates their functions
- 2015
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Ingår i: Immunology and Cell Biology. - : Wiley. - 0818-9641 .- 1440-1711. ; 93:6, s. 581-590
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Tidskriftsartikel (refereegranskat)abstract
- Signaling lymphocytic activation molecule (SLAM) receptors have an important role in the development of immune responses because of their roles, for exampe, in NK cell cytotoxicity and cytokine production by NK, T cells and myeloid cells. The SLAM receptor CD244 (2B4, SLAMf4) is expressed on a variety of immune cell types but most of its functions have been examined on NK and T cells. In the present study, we investigated expression and function of CD244 in murine subsets of dendritic cells (DCs). We report that all subsets of murine DCs examined expressed CD244, although the expression levels of CD244 varied between subsets. Splenic and resident mesenteric lymph node (MLN) DCs from CD244(-/-) mice expressed lower levels of CD86 and MHC class II compared with wild-type mice. Upon Toll-like receptor (TLR) stimulation, no differences in surface expression of these molecules were observed between DCs from CD244(-/-) and wild-type mice. However, splenic DCs from CD244(-/-) mice upon stimulation with TLR binding ligands lipopolysaccharide (LPS) and CpG produced significantly higher levels of pro-inflammatory cytokines. In addition, DCs from CD244(-/-) mice elicited increased NK cell activation in vitro. These data add CD244 to a growing list of immuno-modulatory receptors found on DCs.
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| 4. |
- Avican, Kemal, et al.
(författare)
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RNA atlas of human bacterial pathogens uncovers stress dynamics linked to infection
- 2021
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Ingår i: Nature Communications. - : Springer Nature. - 2041-1723. ; 12:1
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Tidskriftsartikel (refereegranskat)abstract
- Bacterial processes necessary for adaption to stressful host environments are potential targets for new antimicrobials. Here, we report large-scale transcriptomic analyses of 32 human bacterial pathogens grown under 11 stress conditions mimicking human host environments. The potential relevance of the in vitro stress conditions and responses is supported by comparisons with available in vivo transcriptomes of clinically important pathogens. Calculation of a probability score enables comparative cross-microbial analyses of the stress responses, revealing common and unique regulatory responses to different stresses, as well as overlapping processes participating in different stress responses. We identify conserved and species-specific ‘universal stress responders’, that is, genes showing altered expression in multiple stress conditions. Non-coding RNAs are involved in a substantial proportion of the responses. The data are collected in a freely available, interactive online resource (PATHOgenex).
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| 6. |
- Gomez-Carretero, S., et al.
(författare)
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Correction: Redox-active conducting polymers modulate Salmonella biofilm formation by controlling availability of electron acceptors (vol 3, article number 19, 2017)
- 2018
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Ingår i: npj Biofilms and Microbiomes. - : Nature Publishing Group. - 2055-5008. ; 4:1
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- In the original published article, the author list did not include Karl Svennersten, Kristin Persson, Edwin Jager and Magnus Berggren. After publication, we were notified by the corresponding author that the author list did not accurately reflect the contributions made, and these authors have been added to the author list. The original “Author Contributions” stated that “S.G.C., M.R., and A.R.D. designed research; S.G.C. performed all experiments…” this has been updated to read “S.G.C., K.S., K.M.P., E.W.H.J., M.B., M.R., and A.R.D. designed research; K.S., K.M.P., and E.W.H.J. performed experiments; S.G.C. performed all reported experiments…”. The “Acknowledgements” previously read “We thank K. Svennersten, A. Kader, K. Persson, and M. Berggren for fruitful discussions, and S. Löffler for insightful comments on the manuscript…” and have been updated to state “We thank A. Kader for fruitful discussions and S. Loffler for insightful comments on the manuscript…”. The “Competing Interests” section did not require any amendments. All authors have agreed with this correction statement and authorship change.
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| 7. |
- Gomez-Carretero, S, et al.
(författare)
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Redox-active conducting polymers modulate Salmonella biofilm formation by controlling availability of electron acceptors
- 2017
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Ingår i: NPJ biofilms and microbiomes. - : Springer Science and Business Media LLC. - 2055-5008. ; 3, s. 19-
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Tidskriftsartikel (refereegranskat)abstract
- Biofouling is a major problem caused by bacteria colonizing abiotic surfaces, such as medical devices. Biofilms are formed as the bacterial metabolism adapts to an attached growth state. We studied whether bacterial metabolism, hence biofilm formation, can be modulated in electrochemically active surfaces using the conducting conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT). We fabricated composites of PEDOT doped with either heparin, dodecyl benzene sulfonate or chloride, and identified the fabrication parameters so that the electrochemical redox state is the main distinct factor influencing biofilm growth. PEDOT surfaces fitted into a custom-designed culturing device allowed for redox switching in Salmonella cultures, leading to oxidized or reduced electrodes. Similarly large biofilm growth was found on the oxidized anodes and on conventional polyester. In contrast, biofilm was significantly decreased (52–58%) on the reduced cathodes. Quantification of electrochromism in unswitched conducting polymer surfaces revealed a bacteria-driven electrochemical reduction of PEDOT. As a result, unswitched PEDOT acquired an analogous electrochemical state to the externally reduced cathode, explaining the similarly decreased biofilm growth on reduced cathodes and unswitched surfaces. Collectively, our findings reveal two opposing effects affecting biofilm formation. While the oxidized PEDOT anode constitutes a renewable electron sink that promotes biofilm growth, reduction of PEDOT by a power source or by bacteria largely suppresses biofilm formation. Modulating bacterial metabolism using the redox state of electroactive surfaces constitutes an unexplored method with applications spanning from antifouling coatings and microbial fuel cells to the study of the role of bacterial respiration during infection.
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| 8. |
- Gomez-Carretero, S., et al.
(författare)
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Redox-active conducting polymers modulate Salmonella biofilm formation by controlling availability of electron acceptors (vol 3, article number 19, 2017)
- 2017
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Ingår i: npj Biofilms and Microbiomes. - : Springer Science and Business Media LLC. - 2055-5008. ; 3
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Tidskriftsartikel (refereegranskat)abstract
- Biofouling is a major problem caused by bacteria colonizing abiotic surfaces, such as medical devices. Biofilms are formed as the bacterial metabolism adapts to an attached growth state. We studied whether bacterial metabolism, hence biofilm formation, can be modulated in electrochemically active surfaces using the conducting conjugated polymer poly(3,4-ethylenedioxythiophene) (PEDOT). We fabricated composites of PEDOT doped with either heparin, dodecyl benzene sulfonate or chloride, and identified the fabrication parameters so that the electrochemical redox state is the main distinct factor influencing biofilm growth. PEDOT surfaces fitted into a custom-designed culturing device allowed for redox switching in Salmonella cultures, leading to oxidized or reduced electrodes. Similarly large biofilm growth was found on the oxidized anodes and on conventional polyester. In contrast, biofilm was significantly decreased (52-58%) on the reduced cathodes. Quantification of electrochromism in unswitched conducting polymer surfaces revealed a bacteria-driven electrochemical reduction of PEDOT. As a result, unswitched PEDOT acquired an analogous electrochemical state to the externally reduced cathode, explaining the similarly decreased biofilm growth on reduced cathodes and unswitched surfaces. Collectively, our findings reveal two opposing effects affecting biofilm formation. While the oxidized PEDOT anode constitutes a renewable electron sink that promotes biofilm growth, reduction of PEDOT by a power source or by bacteria largely suppresses biofilm formation. Modulating bacterial metabolism using the redox state of electroactive surfaces constitutes an unexplored method with applications spanning from antifouling coatings and microbial fuel cells to the study of the role of bacterial respiration during infection.
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