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Sökning: WFRF:(Rivera Diaz del Castillo P. E. J.)

  • Resultat 1-6 av 6
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  • 2017
  • swepub:Mat__t
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  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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  • Zhao, G-H, et al. (författare)
  • Alloy design by tailoring phase stability in commercial Ti alloys
  • 2021
  • Ingår i: Materials Science & Engineering. - : Elsevier BV. - 0921-5093 .- 1873-4936. ; 815
  • Tidskriftsartikel (refereegranskat)abstract
    • The mechanical characteristics and the operative deformation mechanisms of a metallic alloy can be optimised by explicitly controlling phase stability. Here an integrated thermoelastic and pseudoelastic model is presented to evaluate the beta stability in Ti alloys. The energy landscape of beta ->alpha ' /alpha '' martensitic transformation was expressed in terms of the dilatational and transformational strain energy, the Gibbs free energy change, the external mechanical work as well as the internal frictional resistance. To test the model, new alloys were developed by tailoring two base alloys, Ti-6Al-4V and Ti-6Al-7Nb, with the addition of beta-stabilising element Mo. The alloys exhibited versatile mechanical behaviours with enhanced plasticity. Martensitic nucleation and growth was fundamentally dominated by the competition between elastic strain energy and chemical driving force, where the latter term tends to lower the transformational energy barrier. The model incorporates thermodynamics and micromechanics to quantitatively investigate the threshold energy for operating transformation-induced plasticity and further guides alloy design.
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