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Sökning: WFRF:(Robson Timothy 1990)

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1.
  • Ferrand-Drake Del Castillo, Gustav, 1990, et al. (författare)
  • Generic high-capacity protein capture and release by pH control
  • 2020
  • Ingår i: Chemical Communications. - : Royal Society of Chemistry (RSC). - 1364-548X .- 1359-7345. ; 56:44, s. 5889-5892
  • Tidskriftsartikel (refereegranskat)abstract
    • Techniques for immobilization and release of proteins are of general interest but challenging to develop. Here we show a new method for high-capacity (several µg cm-2) immobilization of proteins in polyelectrolyte brushes by multivalent hydrogen bonds. Upon increasing pH, the proteins are fully released with preserved structure and activity.
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2.
  • Malekian, Bita, 1986, et al. (författare)
  • Detecting Selective Protein Binding Inside Plasmonic Nanopores: Toward a Mimic of the Nuclear Pore Complex
  • 2018
  • Ingår i: Frontiers in Chemistry. - : Frontiers Media SA. - 2296-2646. ; 6:December 2018
  • Tidskriftsartikel (refereegranskat)abstract
    • Biosensors based on plasmonic nanostructures offer label-free and real-time monitoring of biomolecular interactions. However, so do many other surface sensitive techniques with equal or better resolution in terms of surface coverage. Yet, plasmonic nanostructures offer unique possibilities to study effects associated with nanoscale geometry. In this work we use plasmonic nanopores with double gold films and detect binding of proteins inside them. By thiol and trietoxysilane chemistry, receptors are selectively positioned on the silicon nitride interior walls. Larger (similar to 150 nm) nanopores are used detect binding of averaged sized proteins (similar to 60 kg/mol) with high signal to noise (>100). Further, we fabricate pores that approach the size of the nuclear pore complex (diameter down to 50 nm) and graft disordered phenylalanine-glycine nucleoporin domains to the walls, followed by titration of karyopherin beta 1 transport receptors. The interactions are shown to occur with similar affinity as determined by conventional surface plasmon resonance on planar surfaces. Our work illustrates another unique application of plasmonic nanostructures, namely the possibility to mimic the geometry of a biological nanomachine with integrated optical sensing capabilities.
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