| 1. |
- Kepka, Cecilia, et al.
(författare)
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Two-step recovery process for tryptophan tagged cutinase : Interfacing aqueous two-phase extraction and hydrophobic interaction chromatography
- 2005
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Ingår i: Journal of Chromatography A. - : Elsevier BV. - 0021-9673 .- 1873-3778. ; 1075:02-jan, s. 33-41
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Tidskriftsartikel (refereegranskat)abstract
- In this work, the interfacing of a poly(ethylene glycol) (PEG)-phosphate aqueous two-phase system with hydrophobic interaction chromatography (HIC) for primary recovery of an intracellular protein was evaluated. As a model protein, a recombinant cutinase furnished with a tryptophan-proline (WP) peptide tag was used and produced intracellularly in Escherichia coli (E. coli). E. coli cell homogenate was partitioned in a two-phase system and the top phase yield, concentration and purity of the tagged ZZ-cutinase-(WP)(4) was evaluated as function of polymer sizes, system pH and phase volume ratio. The partition behaviour of cell debris, total protein and endotoxin was also monitored. In the HIC part, the chromatographic yield and purity was investigated with respect to ligand hydrophobicity, dilution of loaded top phase and elution conditions. Based on the results, a recovery process was demonstrated where a PEG 1500-K-Na phosphate salt aqueous two-phase system was interfaced with a HIC column. The interfacing was facilitated by the Trp-tagged peptide. The tagged ZZ-cutinase-(WP)4 was obtained in a PEG-free phase and purified to > 95% purity according to silver stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels with a total yield of 83% during the two-step recovery process. © 2005 Elsevier B.V. All rights reserved.
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| 2. |
- Koski, Lassi, et al.
(författare)
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Metals in ALS TDP-43 Pathology
- 2021
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Ingår i: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 22:22
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Forskningsöversikt (refereegranskat)abstract
- Amyotrophic lateral sclerosis (ALS), Alzheimer's disease, Parkinson's disease and similar neurodegenerative disorders take their toll on patients, caregivers and society. A common denominator for these disorders is the accumulation of aggregated proteins in nerve cells, yet the triggers for these aggregation processes are currently unknown. In ALS, protein aggregation has been described for the SOD1, C9orf72, FUS and TDP-43 proteins. The latter is a nuclear protein normally binding to both DNA and RNA, contributing to gene expression and mRNA life cycle regulation. TDP-43 seems to have a specific role in ALS pathogenesis, and ubiquitinated and hyperphosphorylated cytoplasmic inclusions of aggregated TDP-43 are present in nerve cells in almost all sporadic ALS cases. ALS pathology appears to include metal imbalances, and environmental metal exposure is a known risk factor in ALS. However, studies on metal-to-TDP-43 interactions are scarce, even though this protein seems to have the capacity to bind to metals. This review discusses the possible role of metals in TDP-43 aggregation, with respect to ALS pathology.
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| 3. |
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| 5. |
- Mark-Herbert, Cecilia, et al.
(författare)
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Urban Planners’ Perspectives on Public Private Partnership for Wooden Multistorey Construction
- 2023
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Ingår i: Journal of Forest Economics. - : Now Publishers. - 1104-6899 .- 1618-1530. ; 38, s. 7-35
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Tidskriftsartikel (refereegranskat)abstract
- Increased awareness of sustainable development in the construction industry and current housing shortages have contributed to market opportunities for wooden multi-storey construction (WMC) in the Nordic countries. However, the market development has been slow despite access to forest products and a political ambition to transform to a low carbon society. The aim of this study is to explain enabling factors for municipalities in Sweden to create institutional conditions that stimulate local market development of WMC. A comparative case study of municipal efforts to establish WMC norms has been conducted pointing to the importance of relationships, in so-called public private partnerships (PPP). These partnerships serve as long-lasting relations in which development processes can manage challenges relating to, for example, lack of professional experience in WMC and dated material understandings of wood as a construction material. In the Sustainable Development Goals, PPP represent enabling conditions for sustainable system transformations.
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| 6. |
- Mark-Herbert, Cecilia, et al.
(författare)
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Wooden multi-story construction : market development with consumer knowledge, municipal goals and industrial strategies
- 2023
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Ingår i: Fakta. Skog. - 1400-7789.
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Annan publikation (populärvet., debatt m.m.)abstract
- This Forest Fact sheet will present the state of the art of wooden multi-storey construction market development in Finland and Sweden. From a consumer perspective, housing requirements were captured in three factors: environmental and social sustainability, quality, and design. A literature review points to the importance of cost efficiency gains from prefabrication. Municipal perspectives clearly illustrate how political goals may influence the governance and perception of public procurement processes. A transition to sustainable construction practises is supported by legislation that requires sustainability declarations.
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| 7. |
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| 8. |
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Material of movement and thought : reflections on the dancers´s practice and corporeality
- 2013
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Samlingsverk (redaktörskap) (refereegranskat)abstract
- The articles in this volume have grown out of a research project entitled "FromMovement out of Reflection in Becoming: The Dancer and the Creative Process",gathering both professional dancers and theoreticians, and funded by theSwedish Research Council. The overall goal of the project was to approach anunderstanding and a conceptualization of the artistic process of the dancer, takingthe work in the creation ofina Christe!Johannessen's choreographic pieceNOW SHE KNOWS as a point of departure.The focus was both that of the dancer from within the process, and that ofexterna! observers. These viewpoints were not deadlocked hut rather aimed atan interaction and a dialogue between the theoretical and the practical levels.The research methods grew out of an at times quite intimate teamwork wherethe participants' different approaches came to cross and intersect.A number of issues were explored: How does the dancer work in the processwhere the dance takes shape? How does the understanding of a movementmaterial shift through the actual performing of it? What is it to understandor experience a movement from the perspective of the performer? What is itto understand or experience a movement from the perspective of the spectator?What kind of body is the dan ing body and how can it create a variety ofmeanings? Through what concepts are we to think the dancer's practice andcorporeality?The anthology contains essays by Cecilia Roos, Anna Petronella Foultier,Chrysa Parkinson, Katarina Elam, Cecilia Sjöholm, and Irene Hultman.
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| 9. |
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Månsalong : "Tiden är ur led, hjälp oss att vrida den rätt igen”
- 2024
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Konstnärligt arbete (refereegranskat)abstract
- I en serie salonger möts scenkonst, musik, samtal och workshops som undersöker kvinnors bärande roll, tankar, kollektiv och konstnärskap i historiska esoteriska rörelser. Den andra salongen arrangeras på Historiska museet.Initiativtagare är Cecilia Roos, vicerektor för forskning och professor i konstnärliga praktiker på Stockholms Konstnärliga Högskola och Tilde Björfors, regissör, forskare och konstnärlig ledare som bland annat grundat Cirkus Cirkör. Salongerna arrangeras i samarbete med museet Stockholms Kvinnohistoriska.
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| 10. |
- Nagy, Emil, et al.
(författare)
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Unlocking success: key elements of sustainable business models in the wooden multistory building sector
- 2024
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Ingår i: Frontiers in Sustainability. - 2673-4524. ; 5
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Tidskriftsartikel (refereegranskat)abstract
- Few studies have investigated the processes and strategic interactions among the Wooden Multistory Construction (WMC) industry actors, its customers, and local policymakers. Further insights into this field are needed for a sustainability transition in the construction sector. This study aims to explain WMC’s corporate sustainability strategies for co-creation with customers and municipalities. This was based on interviews with key informants in the sector and WMC actors using three case studies in Sweden. Our interviews focused on business processes, interactions with users and municipalities, sustainability, and innovation in the WMC market. The findings reflect the notion of a slow transition in the Swedish WMC market toward using more wood as a bearing structure, which is supported by the competitive advantages and climate performance of wood. The three case studies indicated that the corporate focus is currently placed on incremental improvements in operations, price competitiveness, and successful project management. However, in contexts where wood construction is seen as a unique advantage, collaborations between the construction industry and end users have developed and served as novel platforms for WMC market development. The study concludes that WMCl growth hinges on the industry’s housing offerings prioritizing quality, affordability, and swift construction.
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