| 1. |
- Denoyes-Rothan, B, et al.
(författare)
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QTL analysis for resistances to Colletotrichum acutatum and Phytophthora cactorum in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: Proceedings of the XIth Eucarpia Symposium on Fruit Breeding and Genetics, Vols 1 and 2. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 147-151
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Konferensbidrag (refereegranskat)abstract
- Colletotrichum acutatum and Phytophthora cactorum are two of the most important pathogens of strawberry in Europe, and particularly in France. The characterization of quantitative trait loci (QTL) controlling resistances to these two pathogens would allow a better knowledge of the genetic architecture of these resistances in order to develop durable resistance. A pseudo-testcross strawberry population derived from the cross between Capitola and CF1116 was used for detecting QTLs associated with C. acutatum and P cactorum resistances. One hundred and eighty five individuals were inoculated with C. acutatum by dipping cold stored plants in a conidial suspension adjusted to 2.10(6) conidia per ml. They were also inoculated separately with P cactorum by inserting an agar disk containing mycelium into the crown. Each inoculation was conducted twice. Putative QTLs for resistance to C acutatum and P cactorum were identified by Interval Mapping (Mapmaker-QTL) using a LOD threshold of 2.
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| 2. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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QTL analysis for fruit quality traits and resistance to Colletotrichum acutatum and Phytophthora cactorum in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: PROCEEDINGS OF THE EURO BERRY SYMPOSIUM - COST 836 FINAL WORSKHOP. - : INT SOC HORTICULTURAL SCIENCE. ; , s. 93-97
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Konferensbidrag (refereegranskat)abstract
- Mapping of quantitative trait loci (QTL) controlling components of strawberry (Fragaria x ananassa Duch., 2n=8x=56) fruit quality and resistance to Colletotrichum acutatum and Phytophthora cactorum can be used to provide a better understanding of the genetic control of the traits, and to develop marker assisted selection for breeders. A segregating population of 213 individuals of a cross between ‘Capitola’ and ‘CF1116’, two genotypes with many contrasting fruit quality traits, was used for genetic mapping. ‘Capitola’ was resistant to the C. acutatum pathogenicity group 1 (pg1) and to P. cactorum while ‘CF1116’ was susceptible to C. acutatum and moderately susceptible to P. cactorum. Fruit quality was evaluated on a total of 34 traits, including developmental and fruit aspect related traits, texture related traits, fruit acidity, sugars, ascorbate and amino acid concentrations. Most of the traits except the amino acid concentrations were evaluated during two successive years. Disease responses to C. acutatum (pg2) and P. cactorum were evaluated using a scale from 0 (no observed symptom) to 5 (dead plant) in two successive independent experiments. Data were analysed for putative QTLs using MapQTL. For the fruit quality traits, a total of 22 significant QTLs were detected by simple interval mapping (LOD>3.0) in the first year of analyses and 17 were detected in the second year. Only two QTLs could be detected in both years. The percentages of phenotypic variance explained by each QTL ranged from 6.5% to 16.0%. For resistances, a total of nine QTLs was detected (LOD>2.0), 4 for resistance to C acutatum and 5 for resistance to P. cactorum with the percentage of variation explained by a QTL ranging from 6.5 to 12.2%. No QTL for the P. cactorum resistance overlapped the QTLs for the C acutatum resistance.
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| 3. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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QTL analysis for fruit quality traits in octoploid strawberry (Fragaria x ananassa)
- 2004
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Ingår i: PROCEEDINGS OF THE XITH EUCARPIA SYMPOSIUM ON FRUIT BREEDING AND GENETICS, VOLS 1 AND 2. - : INT SOC HORTICULTURAL SCIENCE. ; , s. 331-335
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Konferensbidrag (refereegranskat)abstract
- Mapping of quantitative trait loci (QTL) controlling components of strawberry (Fragaria x ananassa Duch., 2n=8x=56) fruit quality can be used to provide a better understanding of their genetic control, and to develop marker assisted selection for breeders. For this purpose, a segregating population of 213 individuals of a cross between ‘Capitola’ and CF1116, two genotypes with many contrasting fruit quality traits, was used for genetic mapping. A total of 34 traits involved in fruit quality were evaluated, including developmental and fruit aspect related traits, texture related traits, fruit acidity, sugar and ascorbate concentrations. Amino acid concentrations were quantified using one-dimensional proton NMR spectroscopy. Most of the traits except the amino acid concentrations were evaluated during two successive years. A total of 22 significant QTLs were detected by simple interval mapping (LOD > 3.0) in year 1, four on the female map and 18 on the male map, whereas 17 were detected in year 2, ten on the female map and seven on the male map. Only two QTLs could be detected in both years. When removing the year effect, 22 QTLs were observed, eight on the female and 14 on the male map. The percentages of phenotypic variance explained by each QTL ranged from 6.5% to 16.0%.
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| 4. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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QTL analysis for sugars and organic acids in strawberry fruits
- 2006
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Ingår i: Proceedings of the Vth International Strawberry Symposium. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 573-577
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Konferensbidrag (refereegranskat)abstract
- Improving fruit quality is an important goal for strawberry breeders. Quantitative trait loci (QTL) for fruit quality of cultivated strawberry (Fragaria x ananassa Duch.) have been identified. A segregating population of 213 individuals of a cross between ‘Capitola’ and ‘CF1116’, two genotypes with many contrasting fruit quality traits, was used in the study. Eleven traits related to sugar and acid content were evaluated for all progeny and for both parents. These traits were quantified by enzymatic and metabolic profiling using one-dimensional H-1-NMR. Depending on the trait, measurements were taken over two or three years. Progeny showed a large range of variation for most of the traits. The strongest positive correlations were observed between fructose and glucose contents, and citrate and sum of organic acids. A total of 30 significant QTLs were detected by composite interval mapping (LOD superior to LODthreshold =2.88) and spread over eight female linkage groups and seven male linkage groups in coupling phase with a percentage of variation explained by a QTL ranging from 5.7 to 14.1%. Strong co-locations of putative QTLs for fructose and glucose were detected, while other QTLs were detected for sucrose.
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| 5. |
- Lerceteau-Köhler, Estelle, et al.
(författare)
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The use of molecular markers for durable resistance breeding in the cultivated strawberry (Fragaria x ananassa)
- 2002
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Ingår i: PROCEEDINGS OF THE FOURTH INTERNATIONAL STRAWBERRY SYMPOSIUM, VOLS 1 AND 2. - : INTERNATIONAL SOCIETY HORTICULTURAL SCIENCE. ; , s. 615-618
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Konferensbidrag (refereegranskat)abstract
- In France, particularly in the southwestern area, most damage to the cultivated strawberry is anthracnose, caused by Colletotrichum acutatum. Anthracnose is a polycyclic disease for which no satisfactory disease control method has been developed. Therefore, the objective of our project is to construct durable resistance by combining the different genetic components of resistance to lower both the level of damage and the use of chemicals. Genetic components will be selected by using molecular markers linked to these loci. For monogenic resistance to the pathogenicity group 2 of C. acutatum, bulk segregant analysis (BSA) combined with the AFLP markers has been developed. Four markers were found linked to the dominant gene with a recombination percentage ranging from 0 to 13.9%. For complex resistance to the pathogenicity group 1 of C. acutatum, a linkage map has been constructed in order to detect putative QTLs associated with this complex resistance. The female map was comprised of 31 SDRF (Single Dose Restriction Fragment) markers assigned to 11 linkage groups giving a total size of 392.9 cM. On the male map, 52 SDRF markers mapped on 17 linkage groups giving a total size of 406.9 cM. The work is being continued with the addition of more markers on the maps.
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