| 1. |
- Rumjantseva, Viktoria, 1978
(författare)
-
Dual roles for hepatic lectin receptors in the clearance of chilled platelets
- 2009
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Platelets for transfusion are stored at room temperature because refrigerated platelets are cleared rapidly from circulation. Room temperature storage causes major inconveniences for blood banks and medical risks for recipients. To address this clinically important problem a macrophage-dependent clearance mechanism was defined for platelets refrigerated < 4 h in buffer. Short-term platelet cooling (< 4 h) leads to exposure of βGlcNAc residues on GPIbα. Exposed βGlcNAc are recognized by lectin domains on hepatic macrophages αMβ2, which leads to increased clearance. Capping of exposed βGlcNAc by galactosylation using endogenous galactosyl transferases prevented clearance of short-term cooled platelets. In a follow up clinical study, galactosylation did not improve the circulation of human platelets stored in plasma at 4ºC for > 48 h (Paper 1). To investigate this different outcome we developed a mouse model for studies of storage of platelet-rich plasma (PRP) at 4°C for 48 h. In vitro characterization of the 4°C stored mouse platelets showed similar changes as measured on human platelets after 48 h of refrigeration. Importantly, using the mouse model we demonstrated that galactosylation has no effect on the survival of > 48 h long-term refrigerated mouse platelets (Paper 1). Using the mouse platelet storage model we dissected the clearance mechanism of platelets stored for > 48 h in plasma at 4ºC, showing that 48 h refrigerated platelets are removed by an additional macrophage-independent mechanism, i.e. by the hepatic galactose-dependent lectin the Ashwell-Morell Receptor (AMR) (Paper 2). This conclusion was based on the following evidence: (a) macrophage depletion in mice dramatically improved the circulation of 4 h chilled platelets, but not clearance of 48 h refrigerated platelets; (b) streptavidin-POD staining revealed the localization of long-term refrigerated biotinylated platelets in hepatocytes; (c) 48 h refrigeration tremendously increased the binding of the βgalactose-recognizing lectin RCA-I to platelets; (d) KO mice lacking Asgpr-1 or Asgpr-2 subunits of the AMR supported 48 h refrigerated platelet circulation; (e) co-injection of asialofetuin, a competitive inhibitor of the AMR, restored the survival of 48 h refrigerated platelets but not of isolated platelets stored for <4 h; (f) ingestion of fluorescently labeled long-term refrigerated platelets in a hepatocyte culture was prevented by asialofetuin. Circulation of 48 h platelets was markedly improved by removal of the N-terminal domain of GPIbα using O-sialopeptidase (Paper 2). Platelets from mice lacking the sialyltransferase ST3GalIV were used to investigate the increased clearance of platelets with decreased sialylation. We demonstrated that platelets with increased galactose exposure due to the lack of ST3GalIV expression are also removed by a macrophage-independent mechanism, through AMRs on hepatocytes (Paper 3). In conclusion, our results defined a new hepatic-based clearance mechanism for desialylated platelets, representing the first example of blood cell removal by a non-myeloid cell.
|
|
| 2. |
- Rumjantseva, Viktoria, 1978, et al.
(författare)
-
Dual roles for hepatic lectin receptors in the clearance of chilled platelets.
- 2009
-
Ingår i: Nature medicine. - : Springer Science and Business Media LLC. - 1546-170X .- 1078-8956. ; 15:11, s. 1273-80
-
Tidskriftsartikel (refereegranskat)abstract
- Rapid chilling causes glycoprotein-Ib (GPIb) receptors to cluster on blood platelets. Hepatic macrophage beta(2) integrin binding to beta-N-acetylglucosamine (beta-GlcNAc) residues in the clusters leads to rapid clearance of acutely chilled platelets after transfusion. Although capping the beta-GlcNAc moieties by galactosylation prevents clearance of short-term-cooled platelets, this strategy is ineffective after prolonged refrigeration. We report here that prolonged refrigeration increased the density and concentration of exposed galactose residues on platelets such that hepatocytes, through Ashwell-Morell receptor binding, become increasingly involved in platelet removal. Macrophages rapidly removed a large fraction of transfused platelets independent of their storage conditions. With prolonged platelet chilling, hepatocyte-dependent clearance further diminishes platelet recovery and survival after transfusion. Inhibition of chilled platelet clearance by both beta(2) integrin and Ashwell-Morell receptors may afford a potentially simple method for storing platelets in the cold.
|
|
