| 1. |
- Uhlén, Mathias, et al.
(författare)
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A human protein atlas for normal and cancer tissues based on antibody proteomics
- 2005
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
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Tidskriftsartikel (refereegranskat)abstract
- Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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| 2. |
- Jernberg, Hugo, et al.
(författare)
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Getting Started with Chaos Engineering – design of an implementation framework in practice
- 2020
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Ingår i: Proceedings of the ACM/IEEE International Symposium on Empirical Software Engineering and Measurement (ESEM) Industry Track. - New York, NY, USA : ACM. - 9781450375801
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Konferensbidrag (refereegranskat)abstract
- Background. Chaos Engineering is proposed as a practice to verify a system’s resilience under real, operational conditions. It employs fault injection, is originally developed at Netflix, and supported by several tools from there and other sources. Aims. We aim to intro- duce Chaos Engineering at ICA Gruppen AB, a group of companies whose core business is grocery retail, to improve their systems’ resilience, and to capture our knowledge gained from literature and interviews in a process framework for the introduction of Chaos Engineering. Method. The research is conducted under the design science paradigm, where the problem is conceptualized through a literature study of Chaos Engineering and exploratory interviews in the company. The solution framework is designed based on the literature and a tool survey, and validated by letting software en- gineers at ICA apply parts of it to the software systems of ica.se website, including its e-shop. Results. The main contributions are a synthesis of Chaos Engineering literature and tools, in depth un- derstanding of the needs of the case company, and guidelines for introducing Chaos Engineering. Conclusions. The applied parts were concluded to be feasible and they successfully discovered a set of initial improvement opportunities for the system’s resilience, as well as a suitable Chaos Engineering practice for future resilience testing of the system. We recommend companies using the frame- work as a guide for the implementation of Chaos Engineering.
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| 3. |
- Lindén, Mårten, et al.
(författare)
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Tumour expression of bladder cancer-associated urinary proteins
- 2013
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Ingår i: BJU International. - 1464-4096 .- 1464-410X. ; 112:3, s. 407-415
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Tidskriftsartikel (refereegranskat)abstract
- WHAT'S KNOWN ON THE SUBJECT? AND WHAT DOES THE STUDY ADD?:The current basis for diagnosis and prognosis in urinary bladder cancer is based on the pathologists' assessment of a biopsy of the tumour. Urinary biomarkers are preferable as they can be non-invasively sampled. Urinary cytology is the only test with widespread use but is hampered by poor reproducibility and low sensitivity.By studying the protein expression in bladder tumour tissue samples of proteins previously found in elevated levels in the urine of patients with bladder cancer, we have been able to show that these proteins originate from the tumour. The immunoreactivity of three of the investigated proteins increased with higher stage. Also a serine peptidase inhibitor was found to be predictive of progression from non-muscle-invasive to muscle-invasive tumours.OBJECTIVES:To analyse the expression of five bladder cancer-associated urinary proteins and investigate if expression is related to the malignant phenotype of the tumour.To explore the possible prognostic value of these proteins.PATIENTS AND METHODS:Urine samples, 16 from patients with bladder cancer and 26 from controls, were used in Western Blotting experiments.Tissue microarrays with bladder tissue from 344 patients diagnosed with bladder cancer between 1984 and 2005 was used in immunohistochemistry experiments.The proteins apolipoprotein E (APOE), fibrinogen β chain precursor (FGB), leucine-rich α2-glycoprotein (LRG1), polymerase (RNA) I polypeptide E (POLR1E), α1-antitrypsin (SERPINA1) and topoisomerase 2A (TOP2A) were probed with antibodies validated by the Human Protein Atlas.RESULTS:Increased expressions of APOE, FGB and POLR1E were correlated with increased tumour stage (P < 0.001).Expression of SERPINA1 in Ta and T1 tumours was found to increase the risk of tumour progression (hazard ratio 2.57, 95% confidence interval 1.13-5.87; P = 0.025)CONCLUSIONS: All proteins previously detected in urine from patients with bladder cancer were also expressed in bladder cancer tissue.The expression of APOE, FGB and POLR1E increased with stage and they are potential diagnostic markers.SERPINA1 was identified as a prognostic marker candidate.
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