| 1. |
- Biscaia, S. M. P., et al.
(författare)
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Safe therapeutics of murine melanoma model using a novel antineoplasic, the partially methylated mannogalactan from Pleurotus eryngii
- 2017
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Ingår i: Carbohydrate Polymers. - : ELSEVIER SCI LTD. - 0144-8617 .- 1879-1344. ; 178, s. 95-104
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Tidskriftsartikel (refereegranskat)abstract
- A heteropolysaccharide was isolated by cold aqueous extraction from edible mushroom Pleurotus eryngii ("King Oyster") basidiocarps and its biological properties were evaluated. Structural assignments were carried out using mono-and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. A man-nogalactan having a main chain of (1 -> 6)-linked alpha-D-galactopyranosyl and 3-O-methyl-alpha-D-galactopyranosyl residues, both partially substituted at OH-2 by beta-D-Manp (MG-Pe) single-unit was found. Biological effects of mannogalactan from P. eryngii (MG-Pe) were tested against murine melanoma cells. MG-Pe was non-cytotoxic, but reduced in vitro melanoma cells invasion. Also, 50 mg/kg MG-Pe administration to melanoma-bearing C57BL/6 mice up to 10 days decreased in 60% the tumor volume compared to control. Additionally, no changes were observed when biochemical profile, complete blood cells count (CBC), organs, and body weight were analyzed. Mg-Pe was shown to be a promising anti-melanoma molecule capable of switching melanoma cells to a non-invasive phenotype with no toxicity to melanoma-bearing mice.
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| 2. |
- Ruthes, Andrea C., et al.
(författare)
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Comparative Recalcitrance and Extractability of Cell Wall Polysaccharides from Cereal (Wheat, Rye, and Barley) Brans Using Subcritical Water
- 2020
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Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 8:18, s. 7192-7204
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Tidskriftsartikel (refereegranskat)abstract
- Cereal brans constitute an unexploited source of valuable cell wall polysaccharides and phenolics. Sequential subcritical water extraction (SWE) of wheat, barley, and rye bran enables the isolation of cell wall polysaccharides with targeted molecular structures. The extracts switched from glucan-rich to higher contents of feruloylated arabinoxylan (F-AX) with extraction time, resulting in significant radical scavenging activity. Structurally, wheat and rye F-AXs exhibited mostly single C(O)-3 arabinose substitutions, whereas barley bran F-AX showed a complex substitution pattern. The recalcitrance of the insoluble residues after SWE was revealed by the enrichment of F-AX populations with disubstituted arabinose units and the presence of hydroxycinnamic acids, lignin, and proteins. This study demonstrates the versatility of SWE to extract cell wall polysaccharides from cereal brans with tailored molecular structures and radical scavenging activity for potential use in food and biomedical applications.
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| 3. |
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| 4. |
- Rudjito, Reskandi C., et al.
(författare)
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Feruloylated Arabinoxylans from Wheat Bran : Optimization of Extraction Process and Validation at Pilot Scale
- 2019
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Ingår i: ACS Sustainable Chemistry and Engineering. - : American Chemical Society (ACS). - 2168-0485. ; 7:15, s. 13167-13177
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Tidskriftsartikel (refereegranskat)abstract
- The extraction of feruloylated arabinoxylan (AX) from wheat bran was optimized in terms of the pretreatments prior to subcritical water extraction and then validated at the pilot scale. Destarching of wheat bran was critical to improve the yields and purity of the extracted AX. Time evolution profiles from sequential extractions revealed differences in the extractability of different polysaccharides, whereby beta-D-glucans were first extracted followed by AX. At longer extraction times, the AX population was more substituted and more uniform in terms of molar mass distribution. Validation of the process at pilot scale (with a scaling factor of 33) resulted in AXs with similar molecular characteristics as in the laboratory scale. Both solid to liquid ratio and extraction time were essential in improving the yields. Our study demonstrated scalability of the process, opening opportunities for the further development of multifunctional food and material products from the extracted AXs.
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| 5. |
- Amaral, Sarah da Costa, et al.
(författare)
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Cytotoxic effect of crude and purified pectins from Campomanesia xanthocarpa Berg on human glioblastoma cells
- 2019
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Ingår i: Carbohydrate Polymers. - : ELSEVIER SCI LTD. - 0144-8617 .- 1879-1344. ; 224
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Tidskriftsartikel (refereegranskat)abstract
- A new source of pectin with a cytotoxic effect on glioblastoma cells is presented. A homogeneous GWP-FP-S fraction (M-w, of 29,170 g mol(-1)) was obtained by fractionating the crude pectin extract (GW) from Campomanesia xanthocarpa pulp. According to the monosaccharide composition, the GWP-FP-S was composed of galacturonic acid (58.8%), arabinose (28.5%), galactose (11.3%) and rhamnose (1.1%), comprising 57.7% of homogalacturonans (HG) and 42.0% of type I rhamnogalacturonans (RG-I). These structures were characterized by chromatographic and spectroscopic methods; GW and GWP-FP-S fractions were evaluated by MIT and crystal violet assays for their cytotoxic effects. Both fractions induced cytotoxicity (15.55-37.65%) with concomitant increase in the cellular ROS levels in human glioblastoma cells at 25-400 mu g mL(-)(1), after 48 h of treatment, whereas no cytotoxicity was observed for normal NIH 3T3 cells. This is the first report of in vitro bioactivity and the first investigation of the antitumor potential of gabiroba pectins.
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| 6. |
- Barbieri, Shayla Fernanda, et al.
(författare)
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Extraction, purification and structural characterization of a galactoglucomannan from the gabiroba fruit (Campomanesia xanthocarpa Berg), Myrtaceae family
- 2017
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Ingår i: Carbohydrate Polymers. - : Elsevier BV. - 0144-8617 .- 1879-1344. ; 174, s. 887-895
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Tidskriftsartikel (refereegranskat)abstract
- In this study, we isolated and structurally characterized, for the first time, a galactoglucomannan (GGM) from the pulp of gabiroba, a Myrtaceae family species. The HPSEC-MALLS-RI analysis showed a homogeneous polysaccharide with molar mass of 25,340 g mol(-1). The monosaccharide composition showed that the GGM consisted of Man:Glc:Gal in a molar ratio of 1:1:0.6. Methylation and 1D and 2D NMR analyses suggested that the main chain of the GGM consisted of beta-D-Glcp and beta-D-Manp units (1 -> 4)-linked. The alpha-D-Galp substitutions occur mainly at O-6 position of beta-D-Manp units. The glycosidic linkages of the GGM were evident by the presence of the characteristic signals of 4-O-substituted residues at delta 78.6/3.69 for both beta-D-Glcp and beta-D-Manp. Furthermore, the 0-6 substitutions for both beta-D-Glcp and beta-D-Manp units were confirmed by signals at delta 67.1/4.00 and 3.93. The interglycosidic correlations, obtained through the analysis of the HMBC spectrum, further confirm the structure. (C) 2017 Elsevier Ltd. All rights reserved.
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| 7. |
- Bhattacharya, Abhishek, et al.
(författare)
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Enzyme synergy for the production of arabinoxylo-oligosaccharides from highly substituted arabinoxylan and evaluation of their prebiotic potential
- 2020
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Ingår i: Lebensmittel-Wissenschaft + Technologie. - : Elsevier BV. - 0023-6438 .- 1096-1127. ; 131
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Tidskriftsartikel (refereegranskat)abstract
- Wheat bran arabinoxylan can be converted by enzymatic hydrolysis into short arabinoxylo-oligosaccharides (AXOS) with prebiotic potential. Alkali extraction of arabinoxylan from wheat-bran offers advantages in terms of yield and results in arabinoxylan with highly-substituted regions which has been a challenge to hydrolyse using endoxylanases. We show that this hurdle can be overcome by selecting an arabinoxylanase that attacks these regions. The yield of AXOS can be increased by enzyme synergy, involving the hydrolysis of some arabinoxylan side groups. Thus, arabinoxylanase (CtXyl5At) from Clostridium thermocellum, belonging to subfamily 34 of glycoside hydrolase (GH) family 5 was investigated pertaining to its specificity for highly-substituted regions in the arabinoxylan-backbone. CtXyl5At preferentially hydrolysed the water-soluble fraction of alkali-extracted arabinoxylan. AXOS with DP 2-4 were determined as major products from CtXyl5At catalyzed hydrolysis. Increase in AXOS yield was observed with enzyme synergy, involving an initial treatment of soluble arabinoxylan with a GH43 alpha-L-arabinofuranosidase from Bifidobacterium adolescentis termed BaAXHd3 (30 degrees C, 6h), followed by hydrolysis with CtXyl5At (50 degrees C, 24h). The prebiotic potential of AXOS was shown by growth analysis using the human gut bacteria Bifidobacterium adolescentis ATCC 15703 and Roseburia hominis DSM 6839. Importantly, AXOS were utilized by the bacteria and short-chain fatty acids were produced.
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| 8. |
- Cantu-Jungles, Thaisa Moro, et al.
(författare)
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In vitro fermentation of Cookeina speciosa glucans stimulates the growth of the butyrogenic Clostridium cluster XIVa in a targeted way
- 2018
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Ingår i: Carbohydrate Polymers. - : Elsevier. - 0144-8617 .- 1879-1344. ; 183, s. 219-229
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Tidskriftsartikel (refereegranskat)abstract
- Dietary fiber chemical and physical structures may be critical to the comprehension of how they may modulate gut bacterial composition. We purified insoluble polymers from Cookeina speciosa, and investigated its fermentation profile in an in vitro human fecal fermentation model. Two glucans, characterized as a (1 -> 3),(1 -> 6)-linked and a (1 -> 3)-linked beta-D-glucans were obtained. Both glucans were highly butyrogenic and propiogenic, with low gas production, during in vitro fecal fermentation and led to distinct bacterial shifts if compared to fructooligosaccharides. Specific increases in Bacteroides uniformis and genera from the Clostridium cluster XIVa, such as butyrogenic Anaerostipes and Roseburia were observed. The (1 -> 3)-linked beta-D-glucan presented a faster fermentation profile compared to the branched (1 -> 3),(1 -> 6)-linked beta-D-glucan. Our findings support the view that depending on its fine chemical structure, and likely its insoluble nature, these dietary fibers can be utilized to direct a targeted promotion of the intestinal microbiota to butyrogenic Clostridium cluster XIVa bacteria.
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| 9. |
- de Jesus, Liana Inara, et al.
(författare)
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Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus
- 2018
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Ingår i: International Journal of Biological Macromolecules. - : Elsevier. - 0141-8130 .- 1879-0003. ; 117, s. 1361-1366
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Tidskriftsartikel (refereegranskat)abstract
- A water-soluble beta-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 x 10(5) Da with a random coil conformation for molecular weights below 1 x 10(6) Da. Typical signals of beta-(1 -> 3)-linkages were observed in NMR spectrum (delta 102.7/4.76; 102.8/4.74; 102.9/4.52; and delta 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at delta 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a beta-D-glucan with a main chain (1 -> 3)-linked, substituted at O-6 by single-units of glucose. The beta-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 mu g mL(-1) and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.
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| 10. |
- Martínez-Abad, Antonio, et al.
(författare)
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Enzymatic-assisted extraction and modification of lignocellulosic plant polysaccharides for packaging applications
- 2015
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Ingår i: Journal of Applied Polymer Science. - : John Wiley & Sons. - 0021-8995 .- 1097-4628. ; 133:2
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Tidskriftsartikel (refereegranskat)abstract
- Plant polysaccharides comprise the main renewable resource available in the biosphere for biomaterial production. However, the recalcitrant and heterogeneous structure of lignocellulosic biomass hinders the effective fractionation and exploitation of the polysaccharide components for the design of carbohydrate-based materials. Carbohydrate-active enzymes constitute a selective and versatile biotechnological tool that can assist during the biomass pretreatment steps to extract and fractionate the polysaccharide macromolecular components. Moreover, this enzymatic toolbox can be as well exploited for the tailored modification of the molecular structure of relatively pure polysaccharide components to achieve customized macroscopic properties. This review critically discusses the potential and challenges of the use of plant lignocellulosic polysaccharides and enzymatic modifications to design and prepare suitable materials for packaging applications in terms of their structure-property relations. Structural factors such as the molar mass and crystallinity of the polysaccharide fractions and functional factors such as water sensitivity and processability of the derived films are critical for the material performance.
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