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- Rydberg Sterner, Therese, et al.
(författare)
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The Gothenburg H70 Birth cohort study 2014-16: design, methods and study population.
- 2019
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Ingår i: European journal of epidemiology. - : Springer Science and Business Media LLC. - 1573-7284 .- 0393-2990. ; 34:2, s. 191-209
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Tidskriftsartikel (refereegranskat)abstract
- To improve health care for older persons, we need to learn more about ageing, e.g. identify protective factors and early markers for diseases. The Gothenburg H70 Birth Cohort Studies (the H70 studies) are multidisciplinary epidemiological studies examining representative birth cohorts of older populations in Gothenburg, Sweden. So far, six birth cohorts of 70-year-olds have been examined over time, and examinations have been virtually identical between studies. This paper describes the study procedures for the baseline examination of the Birth cohort 1944, conducted in 2014-16. In this study, all men and women born 1944 on specific dates, and registered as residents in Gothenburg, were eligible for participation (n=1839). A total of 1203 (response rate 72.2%; 559 men and 644 women; mean age 70.5years) agreed to participate in the study. The study comprised sampling of blood and cerebrospinal fluid, psychiatric, cognitive, and physical health examinations, examinations of genetics and family history, use of medications, social factors, functional ability and disability, physical fitness and activity, body composition, lung function, audiological and ophthalmological examinations, diet, brain imaging, as well as a close informant interview, and qualitative studies. As in previous examinations, data collection serves as a basis for future longitudinal follow-up examinations. The research gained from the H70 studies has clinical relevance in relation to prevention, early diagnosis, clinical course, experience of illness, understanding pathogenesis and prognosis. Results will increase our understanding of ageing and inform service development, which may lead to enhanced quality of care for older persons.
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- Arvidsson Rådestig, Maya, et al.
(författare)
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Subtle Differences in Cognition in 70-Year-Olds with Elevated Cerebrospinal Fluid Neurofilament Light and Neurogranin: A H70 Cross-Sectional Study
- 2023
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Ingår i: Journal of Alzheimers Disease. - : IOS Press. - 1387-2877 .- 1875-8908. ; 91:1, s. 291-303
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Tidskriftsartikel (refereegranskat)abstract
- Background: Most research on cerebrospinal fluid (CSF) neurofilament light protein (NfL) as a marker for neurodegeneration and neurogranin (Ng) for synaptic dysfunction has largely focused on clinical cohorts rather than population-based samples. Objective: We hypothesized that increased CSF levels of NfL and Ng are associated with subtle cognitive deficits in cognitively unimpaired (CU) older adults. Methods: The sample was derived from the Gothenburg H70 Birth Cohort Studies and comprised 258 CU 70-year-olds, with a Clinical Dementia Rating score of zero. All participants underwent extensive cognitive testing. CSF levels of NfL and Ng, as well as amyloid beta(1-42), total tau, and phosphorylated tau, were measured. Results: Participants with high CSF NfL performed worse in one memory-based test (Immediate recall, p = 0.013) and a language test (FAS, p = 0.016). Individuals with high CSF Ng performed worse on the memory-based test Supra Span (p = 0.035). When stratified according to CSF tau and A beta(42) concentrations, participants with high NfL and increased tau performed worse on a memory test than participants normal tau concentrations (Delayed recall, p = 0.003). In participants with high NfL, those with pathologic A beta(42) concentrations performed worse on the Delayed recall memory (p = 0.044). In the high Ng group, participants with pathological A beta(42) concentrations had lower MMSE scores (p = 0.027). However, in regression analysis we found no linear correlations between CSF NfL or CSF Ng in relation to cognitive tests when controlled for important co-variates. Conclusion: Markers of neurodegeneration and synaptic pathology might be associated with subtle signs of cognitive decline in a population-based sample of 70-year-olds.
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| 6. |
- Molander, Sverker, 1957, et al.
(författare)
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Calculating the Swedish economy‐wide emissions of additives from plastic materials
- 2012
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Ingår i: 33rd Annual Meeting SETAC North America.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- "Plastics" are very diverse, multipurpose and ubiquitous materials found in very many types of products like shoes, cars, bags and containers. Plastic materials have these many uses due to the possibility of modifying the polymer matrices constituting the bulk material in very many ways with a large number of additives; compounds that are more or less permanently attached in the matrix. Some of these additives have properties with high importance for the final functionality of the product. Flame retardants is a well know example of an additive making otherwise combustible plastic materials (often textile fibres) much less apt for taking fire. There are however a very large number of substances which have got less attention.Our modeling approach aims at a quantification of emissions from a large set of materials occuring in a typical developed country - Sweden. The approach is "bottom-up" in the sense that it is not based on a back-calculation of measured emitted substances. It is rather a combination of a diffusive mass-transfer emission model with models providing information on aggregated product surface areas and material composition of these areas. This combination of the physico-chemical modeling of substance release from a surface under a specific set of environmental conditions, which also rely on substance and material characteristics, and the substance and material flow models, based on trade statistics, longevity data and product properties, is a unique emission model giving the possibility to feed in results to environmental fate modelling and environmental analysis.Results show that textiles, and the huge number of substances occuring in them, are of particular interest due to the fact that this product category constitutes the largest surface area. Due to wear and washing textiles are also fragmented, giving very small fibers and fragments contributing to a further increased surface area that increase the emission rate from the fibre materials in textiles. The result indicate that a number of substances, beside known environmental pollutant, are emitted from textiles.
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- Molander, Sverker, 1957, et al.
(författare)
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Conceptualizing emissions of chemicals from products - foundations of a modelling approach
- 2011
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Ingår i: 21st Annual Meeting SETAC Europe, Milan, Italy, 2011.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The research reported here is approaching the task of conceptually linking chemicals in products to their occurrence in environmental samples building on earlier work applying the concepts of substance and material flow analysis and bridging that modelling with chemical diffusive mass-transfer models. The bridging of these concepts is possible using the “combined nomenclature”(CN) and concepts such as total stock, total product area and mean composition of materials in the surface of products. The process of conceptual modelling used builds on principles used in design of information systems in multidomain user settings. A conceptual model of the information i.e. the nomenclatures, terminologies and categorisations of concepts and terms, has been identified through workshops with researchers, a references group and a literature study. The aim of the model is to cover the processes that lead to emissions from environmental relevant processes of products along the entire “life-cycle”. The core concepts of the technical system are “product” and “use”. The “product” concept hence includes any physical product not limited to a specific category. Each product consists of one or several components, which in turn are made of one or several materials, which consists of one or several chemicals. Different types of properties and descriptions are related to each of the levels of physical representation from product category (CN-categories) to chemical level.The “use” concept is describing an episode during the “life-time” of a given product. The amount of chemical substances emitted from a product depends on the type of use, the length of time it is used in this particular way and the ambient conditions in the direct vicinity where use takes place. The use concept is thus very wide and encompasses the different stages of a product lifecycle which require a thorough definition of “use types” and ambient conditions.A further specification of concepts has been done, covering around 20 concepts and their further relations and connections to data and data sources useful for calculations will be presented.
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- Möller Rydberg, Emilia, et al.
(författare)
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Knowledge support for ankle fractures in the Swedish Fracture Register - a qualitative study of physicians' experiences
- 2022
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Ingår i: Bmc Health Services Research. - : Springer Science and Business Media LLC. - 1472-6963. ; 22:1
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Tidskriftsartikel (refereegranskat)abstract
- Background The aim of this study is to investigate the experiences of physicians presented with a knowledge support system while registering data on ankle fractures in the Swedish Fracture Register. The present study aims to answer the following research questions: center dot "How is receiving knowledge support while registering a fracture in the Swedish Fracture Register experienced by the physicians using it?". center dot "Can a feeling of increased usability of a quality register be achieved by providing the user with real-time feedback?". Methods A total of 20 physicians using the Swedish Fracture Register were recruited using a purposive sampling strategy. Qualitative content analysis was performed on individual semi-structured interviews performed in May and June 2020. Results The present study demonstrates that the knowledge support system in the Swedish Fracture Register was perceived by the physicians as strengthening the evidence base and improving the quality of ankle fracture treatment. The knowledge support system was evaluated as a good tool for validating clinical decisions and managing the information that needs to be processed to make informed decisions. Conclusions The present study affirms that being provided with knowledge support is appreciated by physicians, increase value for work and enhance the initiative to register. The physicians experienced that the knowledge support provided an appreciated validation of the clinical decisions taken and a feeling of improved care. When incorporating knowledge support into an NQR, consideration must be given to physicians' fears of becoming overly reliant on a template and losing control of the clinical base.
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| 10. |
- Rydberg, Johan, 1973-
(författare)
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Protein-protein interactions in model systems : design, control of catalytic activity and biosensor applications
- 2006
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis describes the design of polypeptides, unordered in the monomeric state but capable of folding into helix-loop-helix motifs and dimerise to form four-helix bundles. The goal of the design was to encode them with the capacity to form dimers highly selectively and the ability to carry out molecular functions in the folded state but not in the unordered state, and thus to establish a molecular link between recognition and function. The 42-residue sequences JR2E and JR2K were both shown by CD spectroscopy to adopt unordered conformations under single solute conditions at pH 7 but to form helical conformations in a 1:1 mixture. Analytical ultracentrifugation showed that JR2E and JR2K formed a clean heterodimer and the dissociation constant Kd, measured by CD spectroscopy, was found to be 5 ± 1 μM. Discrimination was enabled by the incorporation of charged residues at the dimer interface in the helical segments of the helix-loop-helix motif. Glutamic acids were incorporated in JR2E and lysines in JR2K, and charge repulsion prevented the monomeric subunits from forming homodimers. In mixtures, however, highly helical heterodimers were formed. The cooperative transition from unordered conformation to heterodimeric four-helix bundle was exploited in the design of a signal response system by incorporating a reactive site, capable of catalysing the hydrolysis of a m-nitrophenyl ester, into the negatively charged polypeptide. In the unfolded state the functionalised polypeptide was virtually inactive but in the folded state, induced by the interaction with JR2K, the substrate was hydrolysed approximately an order of magnitude more efficiently.Interactions between the designed polypeptides and a functionalised polythiophene polymer were studied and it was found that the conformation of the polymer was controlled by the polypeptides, largely by electrostatic interactions. The negatively charged JR2E forced the polymer to adopt a planar conformation while the positively charged JR2K induced a more twisted conformation of the polymer. The spectral changes coupled to the conformational transitions of the polymer were used to measure the binding of human Carbonic anhydrase II by JR2E functionalised with a benzenesulphonamide ligand, in demonstration of its use as a tool for high-throughput screening.JR2E immobilised on gold nanoparticles was shown to form homodimers reversibly under pH control, with affinities large enough to determine the state of aggregation of the gold nanoparticles.
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