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- Damén, Tor, et al.
(författare)
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Effects of different mean arterial pressure targets on plasma volume, ANP and glycocalyx-A randomized trial.
- 2021
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Ingår i: Acta anaesthesiologica Scandinavica. - : Wiley. - 1399-6576 .- 0001-5172. ; 65:2, s. 220-227
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Tidskriftsartikel (refereegranskat)abstract
- Arterial haematocrit (Hct) has been shown to decrease after anaesthesia induction, most probably because of an increased plasma volume (PV). The primary objective was to quantify change in PV if mean arterial pressure (MAP) was kept at baseline level or allowed to decrease to 60mm Hg. Our secondary objective was to evaluate underlying mechanisms of this response.Twenty-four coronary artery bypass patients were randomized to a higher (90mm Hg, intervention group) or lower (60mm Hg, control group) MAP by titration of norepinephrine. During the experimental procedure, no fluids were administered. Baseline PV was measured by 125 I-albumin and the change in PV was calculated from the change in Hct. Changes in MAP, plasma 125 I-albumin, colloid osmotic pressure, albumin, Mid Regional-pro Atrial Natriuretic Peptide (MR-proANP) and endothelial glycocalyx components were measured from baseline to 50minutes after anaesthesia induction.The MAP during the trial was 93±9mm Hg in the intervention group and 62±5mm Hg in the control group. PV increased with up to 420±180mL in the control group and 45±130mL in the intervention group (P<.001). Albumin and colloid osmotic pressure decreased significantly more in the control group. MR-proANP increased in the control group but no shedding of the glycocalyx layer was detected in either of the groups.Allowing mean arterial pressure to fall to 60mm Hg during anaesthesia induction, increases the plasma volume due to reabsorption of interstitial water, with no ANP-induced degradation of the endothelial glycocalyx.
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| 2. |
- Saadati, Sofia, 1992, et al.
(författare)
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Binding and internalization of 177Lu-octreotate in human tumor cell lines of different origin
- 2017
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Ingår i: 63rd Annual Meeting of the Radiation Research Society, Cancun, Mexico.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Peptide Receptor Radionuclide Therapy (PRRT) with 177Lu-octreotate is used for systemic treatment of patients with somatostatin receptor (SSTR)-expressing neuroendocrine tumors (NETs), mainly for small-intestine NETs and endocrine pancreatic tumors. Further research is needed to evaluate the possibility of using this type of treatment in patients with other SSTR-expressing tumors. Tumor binding and uptake of the radiopharmaceutical is highly dependent on SSTR expression. In order to determine the potential of using 177Lu-octreotate for treatment of other tumor cell lines, in vitro studies of binding and internalization are needed. The aim of this study was to asses binding and internalization of 177Lu-octreotate in various cancer cell lines and compare with our previous results. In vitro studies were performed on neuroblastoma (CLB-BAR, IMR-32), lung adenocarcinoma (h1975, h2228) and invasive breast carcinoma (BT474, MCF-7, MDA-MB-231, MDA-MB-361, T47D, ZR-75-1) cell lines. Cell cultures were incubated with low or high amounts of 177Lu-octreotate. To block SSTR and thereby determine the specific uptake, control groups were incubated with 177Lu-octreotate and excess octreotide. The amount of unbound, membrane-bound, and internalized 177Lu in each sample was determined after 24 h. Several of the studied tumor cell lines showed specific binding of 177Lu-octreotate. The highest binding and internalization after 24 h was seen for the neuroblastoma cell lines IMR-32 (58% internalized, 9.4% membrane-bound) and CLB-BAR (26% internalized, 3.4% membrane-bound). Specific binding was also found in some breast cancer cell lines (e.g. 3.1% internalized, 0.5% membrane-bound in MDA-MB-361). No specific binding was found in lung adenocarcinoma. In comparison with our previous findings in NET and NET-like cell lines, these results indicate that SSTR-based PRRT may be a potential treatment option for patients with neuroblastoma and certain types of breast cancer. Promising results showing specific tumor uptake of 177Lu-octreotate were obtained for SSTR-expressing tumor cell lines in vitro, indicating the possibility of using SSTR-based diagnostic and therapeutic regimes on more tumor types than those in current clinical practice.
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| 3. |
- Saadati, Sofia, 1992, et al.
(författare)
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Early indications on UVC-related carcinogenesis: premutagenic DNA damage in mice following 222-nm light exposure
- 2016
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Ingår i: 4th Swedish Cancer Research Meeting, Gothenburg, Sweden, November 7-8, 2016.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Surgical site infections, caused by air borne bacteria and viruses, can have lethal consequences. The use of conventional germicidal UV lamps, emitting primarily 254-nm light, at the surgical site has been prevented by being cancerogenic. Far-UVC light (200-222 nm) has been shown to be equieffective without being as damaging to human cells in vitro. In mammalian skin, it is DNA-damage in the basal cells that contributes to the major risk of cancer induction. The aim was to test the hypothesis that 222-nm light is less premutagenic than 254-nm light in vivo. SKH1 mice were exposed to different doses of 222-nm light and compared with positive (254-nm light) and negative (sham irradiated) controls. The mice were killed after 48h and dorsal skin samples were excised. UV specific DNA-damage cyclobutane pyrimidine dimer (CPD) was assessed by immunohistochemical analysis and a damage depth profile was determined. Loss of light intensity was also determined using phantoms with different protein concentrations, simulating various depths in skin. While the amount of CPD was significantly lower following 222-nm light in comparison with 254-nm light at all epidermal depths, no difference was seen in comparison with negative controls. Absorption of 222-nm light was much higher than 254-nm light. Results show that 222-nm light, for the investigated doses, does not appear to be premutagenic. These promising results indicate the potentials of far-UVC lamps as a disinfecting tool without patient and staff being subject to hazardous exposure.
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