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- Kuchinskaya, Ekaterina, et al.
(författare)
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Interphase fluorescent in situ hybridization deletion analysis of the 9p21 region and prognosis in childhood acute lymphoblastic leukaemia (ALL) : results from a prospective analysis of 519 Nordic patients treated according to the NOPHO-ALL 2000 protocol
- 2011
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Ingår i: British Journal of Haematology. - : Wiley. - 0007-1048 .- 1365-2141. ; 152:5, s. 615-622
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Tidskriftsartikel (refereegranskat)abstract
- Interphase fluorescent in situ hybridization (FISH) was applied on diagnostic BM smears from 519 children with acute lymphoblastic leukaemia (ALL) in order to establish the frequency and prognostic importance of 9p21 deletion in children enrolled in the Nordic Society of Paediatric Haematology and Oncology (NOPHO) - 2000 treatment protocol. Among the patients, 452 were diagnosed with B-cell precursor (BCP)-ALL and 66 with T-ALL. A higher incidence of 9p21 deletions was found in T-ALL (38%) compared to BCP-ALL (15·7%). Homozygous deletions were found in 19·7% of T-ALL and 4·0% of BCP-ALL; hemizygous deletions were found in 18·2% and 11·7% respectively. In our series, 9p21 deletions were detected in all age groups with a steady rise in the frequency with age. There was no significant difference in outcome between cases with or without 9p21 deletion or between cases with hemi- or homozygous deletions of 9p21. In conclusion, in this large series of childhood ALL deletion of 9p21 was not associated with worse prognosis. However, interphase FISH deletion analysis of 9p21 could be used as a first step to detect unfavourable subtle cytogenetic aberrations such as the dic(9;20) rearrangement.
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| 2. |
- Stanezai, Sanga, et al.
(författare)
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Higher intensity of low molecular weight protein tyrosine phosphatase/ ACP-1 in survivors of patients diagnosed with diffuse large B cell lymphoma (DLBCL) compared to non-survivors
- 2016
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Ingår i: Austin Biology. - : Austin Publishing. ; 1:2:2
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Tidskriftsartikel (refereegranskat)abstract
- Adult diffuse large B cell lymphoma (DLBCL) is a heterogeneous form of hematopoietic cancer and difficult to treat. In order to find a better diagnostic indication for the disease, we analyzed low molecular weight protein tyrosine phosphatase (LMWPTP) that in humans is encoded by the ACP1 gene. LMWPTP is an enzyme shown to counteract protein tyrosine kinases (PTK) and was suggested to be a negative growth factor regulator. However, the 18 kDa PTP can also have a positive effect on cell growth and proliferation, indicating a controversial role in the tumorigenic process. LMWPTP exists in different isoforms which are electrophoretically, kinetically and immunologically distinct. We have studied two subgroups of DLBCL consisting of a germinal center B cell like (GCB) and a non-germinal center B cell like (non-GCB) group. The two subgroups have been defined by gene-expressing profiling and are associated with differential outcome. The expression levels of LMWPTP protein was compared and showed significant differences between the GCB and non-GCB subgroups (p=0.012). Interestingly, when the samples were divided into survivors and non-survivors, and thereafter analyzed for LMWPTP expression, the samples from patients with a higher survival rate showed increased staining intensity, whereas the samples from patients with lower intensity of LMWPTP did not survive the disease (p=0.001). In conclusion, we have shown that DLBCL patients with worse outcome express LMWPTP with a lower intensity, suggesting a tumor suppressor role for this form of the enzyme.
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| 4. |
- Wulff Sahlén, Elisabeth, 1969-
(författare)
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Interactive Technology in the FL Classroom : Using Polling and Chat to Promote Student Participation in Campus Education
- 2011
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Ingår i: Conference Proceedings, International Conference ICT for Language Learning, 4th Edition. - Milano : Simonelli Editore - University Press. - 9788876476778
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- This paper presents a project where polling and chat were used in the foreign language classroom to promote student participation and, at the same time, provide a record of student contributions. The setting is a Swedish university course in grammar and translation for advanced learners of English. The majority of seminars are devoted to discussing translation assignments that students have prepared at home. During the project, Adobe Connect Pro, a web conferencing solution for online meetings, was used, and all students had access to a computer in the classroom. For each seminar, an online meeting was set up in Adobe Connect with the following tools enabled: A poll, used as a clicker at the start of the seminar to gather input from students on a specific language issue. A chat, where students typed in their solutions to the translation assignment. A whiteboard, where a selection of student solutions were displayed for feedback and discussion. Student evaluations of the project express that students are positive to using online technology in the classroom. The students participated actively in the poll and chat sessions. In the subsequent oral discussions, participation was remarkably wide. From the teacher’s perspective, the chat provided a written record of student contributions that could be used for further analysis and discussion. Some challenges included managing both written and oral communication in the classroom, and dealing with larger chunks of text in the chat.
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