| 1. |
- Ericsson, Olle, et al.
(författare)
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Clinical validation of a novel automated cell-free DNA screening assay for trisomies 21, 13, and 18 in maternal plasma.
- 2019
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Ingår i: Prenatal diagnosis. - : Wiley. - 1097-0223 .- 0197-3851. ; 39:11, s. 1011-1015
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Tidskriftsartikel (refereegranskat)abstract
- To evaluate clinical performance of a new automated cell-free (cf)DNA assay in maternal plasma screening for trisomies 21, 18, and 13, and to determine fetal sex.Maternal plasma samples from 1200 singleton pregnancies were analyzed with a new non-sequencing cfDNA method, which is based on imaging and counting specific chromosome targets. Reference outcomes were determined by either cytogenetic testing, of amniotic fluid or chorionic villi, or clinical examination of neonates.The samples examined included 158 fetal aneuploidies. Sensitivity was 100% (112/112) for trisomy 21, 89% (32/36) for trisomy 18, and 100% (10/10) for trisomy 13. The respective specificities were 100%, 99.5%, and 99.9%. There were five first pass failures (0.4%), all in unaffected pregnancies. Sex classification was performed on 979 of the samples and 99.6% (975/979) provided a concordant result.The new automated cfDNA assay has high sensitivity and specificity for trisomies 21, 18, and 13 and accurate classification of fetal sex, while maintaining a low failure rate. The study demonstrated that cfDNA testing can be simplified and automated to reduce cost and thereby enabling wider population-based screening.
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| 2. |
- Lindholm, Anna, et al.
(författare)
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Utvärdering av förståelse på skriftlig tentamen
- 2011
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Konferensbidrag (refereegranskat)abstract
- I tidigare studier har det visats att examinationen i hög grad styr hur studenterna kommer att studera under en kurs. Därför är det viktigt att examinationen inte bara testar ytliga kunskaper och beräkningsförmåga, utan även förståelse. I denna artikel diskuteras olika sätt att utvärdera studenternas förståelse genom att analysera resultaten på skriftliga tentamina. En fallstudie på Institutionen för reglerteknik vid Lunds tekniska högskola presenteras och möjliga åtgärder för att öka förståelsen hos studenterna diskuteras.
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| 3. |
- Arndt, Christoph, et al.
(författare)
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Laser Applications to Chemical, Security, and Environmental Analysis : introduction to the feature issue
- 2023
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Ingår i: Applied Optics. - 1559-128X. ; 62:6, s. 1-3
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Tidskriftsartikel (refereegranskat)abstract
- The eighteenth topical meeting on Laser Applications to Chemical, Security, and Environmental Analysis (LACSEA) was held in Vancouver, Canada from 11–15 July 2022, as part of the Optica Optical Sensors and Sensing Congress in a hybrid format allowing on-site and online attendance. The meeting featured a broad range of distinguished papers focusing on recent advances in laser and optical spectroscopy. A total of 52 contributed and invited papers were presented during the meeting, including topics such as photo-acoustic spectroscopy, imaging, non-linear technologies, frequency combs, remote sensing, environmental monitoring, aerosols, combustion diagnostics, hypersonic flow diagnostics, nuclear diagnostics, fs/ps applications, and machine learning and computational sensing.
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| 4. |
- Bang, Charlotte Sahlberg, 1967-, et al.
(författare)
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Multiresistant uropathogenic extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are susceptible to the carbon monoxide releasing molecule-2 (CORM-2).
- 2014
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Ingår i: Microbial Pathogenesis. - London : Elsevier. - 0882-4010 .- 1096-1208. ; 66, s. 29-35
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Tidskriftsartikel (refereegranskat)abstract
- Carbon monoxide (CO) releasing molecules (CO-RMs) have been shown to inhibit growth of commensal Escherichia coli (E. coli). In the present study we examined the effect of CORM-2 on uropathogenic E. coli (UPEC) that produces extended-spectrum β-lactamase (ESBL). Viability experiments showed that CORM-2 inhibited the growth of several different ESBL-producing UPEC isolates and that 500 μM CORM-2 had a bactericidal effect within 4 h. The bactericidal effect of CORM-2 was significantly more pronounced than the effect of the antibiotic nitrofurantoin. CORM-2 demonstrated a low level of cytotoxicity in eukaryotic cells (human bladder epithelial cell line 5637) at the concentrations and time-points where the antibacterial effect was obtained. Real-time RT-PCR studies of different virulence genes showed that the expression of capsule group II kpsMT II and serum resistance traT was reduced and that some genes encoding iron acquisition systems were altered by CORM-2. Our results demonstrate that CORM-2 has a fast bactericidal effect against multiresistant ESBL-producing UPEC isolates, and also identify some putative UPEC virulence factors as targets for CORM-2. CO-RMs may be candidate drugs for further studies in the field of finding new therapeutic approaches for treatment of uropathogenic ESBLproducing E. coli.
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| 5. |
- Bang, Charlotte Sahlberg, 1967-, et al.
(författare)
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The antibacterial effect of nitric oxide against ESBL-producing uropathogenic E-coli is improved by combination with miconazole and polymyxin B nonapeptide
- 2014
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Ingår i: BMC Microbiology. - London : BioMed Central. - 1471-2180. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- Background: Nitric oxide (NO) is produced as part of the host immune response to bacterial infections, including urinary tract infections. The enzyme flavohemoglobin, coded by the hmp gene, is involved in protecting bacterial cells from the toxic effects of NO and represents a potentially interesting target for development of novel treatment concepts against resistant uropathogenic bacteria. The aim of the present study was to investigate if the in vitro antibacterial effects of NO can be enhanced by pharmacological modulation of the enzyme flavohemoglobin.Results: Four clinical isolates of multidrug-resistant extended-spectrum beta-lactamase (ESBL)-producing uropathogenic E. coli were included in the study. It was shown that the NO-donor substance DETA/NO, but not inactivated DETA/NO, caused an initial growth inhibition with regrowth noted after 8 h of exposure. An hmp-deficient strain showed a prolonged growth inhibition in response to DETA/NO compared to the wild type. The imidazole antibiotic miconazole, that has been shown to inhibit bacterial flavohemoglobin activity, prolonged the DETA/NO-evoked growth inhibition. When miconazole was combined with polymyxin B nonapeptide (PMBN), in order to increase the bacterial wall permeability, DETA/NO caused a prolonged bacteriostatic response that lasted for up to 24 h.Conclusion: An NO-donor in combination with miconazole and PMBN showed enhanced antimicrobial effects and proved effective against multidrug-resistant ESBL-producing uropathogenic E. coli.
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| 6. |
- Borggren, Jesper, et al.
(författare)
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Temperature imaging in low-pressure flames using diode laser two-line atomic fluorescence employing a novel indium seeding technique
- 2016
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Ingår i: Applied Physics B. - : Springer Science and Business Media LLC. - 0946-2171 .- 1432-0649. ; 122:3
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Tidskriftsartikel (refereegranskat)abstract
- The use of diode lasers for spatially resolved temperature imaging is demonstrated in low-pressure premixed methane–air flames using two-line atomic fluorescence of seeded indium atoms. This work features the advantages of using compact diode lasers as the excitation sources with the benefits of two-dimensional planar imaging, which is normally only performed with high-power pulsed lasers. A versatile and reliable seeding technique with minimal impact on flame properties is used to introduce indium atoms into the combustion environment for a wide range of flame equivalence ratios. A spatial resolution of around 210 µm for this calibration-free thermometry technique is achieved for three equivalence ratios at a pressure of 50 mbar in a laminar flat flame.
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| 7. |
- Borggren, Jesper, et al.
(författare)
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Temperature imaging in low-pressure flames using diode lasers
- 2014
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Ingår i: Imaging and Applied Optics - Laser Applications to Chemical, Security and Environmental Analysis, LACSEA 2014. - Washington, D.C. : OSA. - 2162-2701. - 9781557523082 - 9781557523082
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Konferensbidrag (refereegranskat)abstract
- We present a calibration free technique for spatially resolved imaging of flame temperature. Its application is demonstrated in a low pressure premixed methane flame seeded with indium. Temperature measurements over a range of equivalence ratios are investigated.
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| 8. |
- Borisov, Vladislav, et al.
(författare)
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Tuning skyrmions in B20 compounds by 4d and 5d doping
- 2022
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Ingår i: Physical Review Materials. - : American Physical Society (APS). - 2475-9953. ; 6:8
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Tidskriftsartikel (refereegranskat)abstract
- Skyrmion stabilization in novel magnetic systems with the B20 crystal structure is reported here, primarily based on theoretical results. The focus is on the effect of alloying on the 3d sublattice of the B20 structure by substitution of heavier 4d and 5d elements, with the ambition to tune the spin-orbit coupling and its influence on magnetic interactions. State-of-the-art methods based on density functional theory are used to calculate both isotropic and anisotropic exchange interactions. Significant enhancement of the Dzyaloshinskii-Moriya interaction is reported for 5d-doped FeSi and CoSi, accompanied by a large modification of the spin stiffness and spiralization. Micromagnetic simulations coupled to atomistic spin-dynamics and ab initio magnetic interactions reveal the spin-spiral nature of the magnetic ground state and field-induced skyrmions for all these systems. Especially small skyrmions similar to 50 nm are predicted for Co0.75Os0.25Si, compared to similar to 148 nm for Fe0.75Co0.25Si. Convex-hull analysis suggests that all B20 compounds considered here are structurally stable at elevated temperatures and should be possible to synthesize. This prediction is confirmed experimentally by synthesis and structural analysis of the Ru-doped CoSi systems discussed here, both in powder and in single-crystal forms.
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| 9. |
- Dennersten, Ulrika, et al.
(författare)
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Lung function in the aging Swedish cystic fibrosis population.
- 2009
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Ingår i: Respiratory medicine. - : Elsevier BV. - 1532-3064 .- 0954-6111. ; 103:7, s. 1076-82
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Tidskriftsartikel (refereegranskat)abstract
- Swedish Cystic Fibrosis (CF) care follows international guidelines in general. The only difference in our CF care package since 25 years is the physiotherapy regimen. Airway clearance therapy has since the early 1980s, from the day of diagnosis, been based upon daily physical exercise in conjunction with techniques for transporting and evacuating mucus. Postural Drainage+/-percussion and vibration has not been used in any age. The aim of this study was to evaluate our CF care package. Lung function data from the start of the study and with a 3-year interval were collected in the entire Swedish CF population > or =7 years old. Data were analysed for the age groups 7-17 and > or =18 years of age. Change of lung function over the study period was calculated. The impact of chronic Pseudomonas aeruginosa (Pa) colonisation and basal FEV(1)%p was also evaluated in a linear mixed model. Data from 99% of the country-wide CF population were available at inclusion. Mean FEV(1)%p was 90+/-21 vs 73+/-26 in the different age groups and mean VC%p was 94+/-18 vs 91+/-20. Forty percent of the adult group was > or =30 years old. Overall, 41% were chronically Pa colonised. Mean annual FEV(1)%p rate of decline was 0.77 and 0.64 in the different age groups. Lung function among Swedish CF patients is good and annual rate of decline low, even in an old cohort. The large proportion of adult patients emphasises future demands on CF care.
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| 10. |
- Edman, Anna, et al.
(författare)
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HOME Vatten i Bottenvikens vattendistrikt. Integrerat modellsystem för vattenkvalitetsberäkningar
- 2008
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- SMHI har utvecklat ett interaktivt modellsystem för vattenkvalitetsberäkningar i mark, sjöar,vattendrag och kustvatten, HOME Vatten. I detta uppdrag har HOME Vatten implementerats iBottenvikens vattendistrikt. De ingående modellerna i HOME Vatten är HBV-NP modellen förmark, sjöar och vattendrag samt Kustzonsmodellen för kustvattnen. Atmosfärsdepositionen bådepå land och i kustområdet beräknas av den atmosfärskemiska MATCH-modellen.HOME Vatten har utvecklats för att vara ett verktyg i svensk vattenförvaltning med speciellt fokuspå EUs ramdirektiv för vatten.Modelluppsättningen i kustvatten har validerats mot tillgängliga mätdata, och visar överlag en godöverrensstämmelse med data. Dock saknas i flera fall mätdata över salthalt och närsalthalter vilketförsvårar möjligheterna att dra några slutsatser om modellkvalitén.Längs delar av kuststräckan förekommer inget kustvattenkontrollprogram, varför modellen inte harkunnat valideras där.
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