| 1. |
- Björkner, Bert, et al.
(författare)
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Allergic contact dermatitis as a complication of lid loading with gold implants.
- 2008
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Ingår i: Dermatitis. - : Mary Ann Liebert Inc. - 1710-3568. ; 19:3, s. 148-153
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Tidskriftsartikel (refereegranskat)abstract
- Paralysis of the orbicularis oculi muscle in patients with a facial nerve injury causes functional and cosmetic disabilities including inability to close the eyelid. This complication is often treated by implanting a gold weight in the upper eyelid, which, however, is not without side effects. Four patients are described who, after lid loading with a gold implant, acquired an inflammatory reaction due to contact allergy to the metal inserted. The allergy was demonstrated by patch testing with gold sodium thiosulfate. The implant was removed and the dermatitis resolved. Contact allergy to gold occurs frequently and may explain many cases of complications to lid loading with the metal.
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| 2. |
- Gustafson, Carl-Johan, et al.
(författare)
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Employing human keratinocytes cultured on macroporous gelatin spheres to treat full thickness-wounds : an in vivo study on athymic rats.
- 2007
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Ingår i: Burns. - : Elsevier BV. - 0305-4179 .- 1879-1409. ; 33:6, s. 726-35
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Tidskriftsartikel (refereegranskat)abstract
- Providing cutaneous wounds with sufficient epidermis to prevent infections and fluid loss is one of the most challenging tasks associated with surgical treatment of burns. Recently, application of cultured keratinocytes in this context has allowed this challenge to be met without several of the limitations connected with the use of split-thickness skin grafts. The continuous development of this novel approach has now revealed that transplantation of cultured autologous keratinocytes as single-cell suspensions exhibits several advantages over the use of cultured epidermal grafts. However, a number of methodological problems remain to be solved, primarily with regards to the complexity of culturing these cells; loss of viability and other negative effects during their preparation and transportation; the relatively long period of time required following transplantation to obtain a sufficiently protective epidermis. In the present investigation we attempted to eliminate these limitations by culturing the keratinocytes on macroporous gelatin spheres. Accordingly, the efficacies of normal human keratinocytes in single-cell suspension or growing on macroporous gelatin spheres, as well as of split-thickness skin grafts in healing wounds on athymic rats were compared. Human keratinocytes were found to adhere and proliferate efficiently both on the surface and within the pores of such spheres. Transplantation of such cells adherent to the spheres resulted in significantly more rapid formation of a stratified epidermis than did transplantation of single-cell suspensions or spheres alone. Twenty-three days after transplantation, the epidermis formed from the cells bound to the spheres was not as thick as the epidermis on wounds covered with split-thickness skin grafts, but significantly thicker than on wounds to which single-cell suspensions, spheres alone or no transplant at all was applied. Furthermore, fluorescence in situ hybridisation revealed that the transplanted keratinocytes, both those adherent to gelatin spheres and those in single-cell suspension, were components of the newly formed epidermis. These findings indicate that application of biodegradable macroporous spheres may prove to be of considerable value in designing cell-based therapies for the treatment of acute and persistent wounds.
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| 3. |
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| 4. |
- Jin, Yuesheng, et al.
(författare)
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Clonal chromosome abnormalities in premalignant lesions of the skin.
- 2002
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Ingår i: Cancer Genetics and Cytogenetics. - 0165-4608. ; 136:1, s. 48-52
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Tidskriftsartikel (refereegranskat)abstract
- Two lesions, actinic keratosis (AK) and squamous cell carcinoma in situ (CIS), are believed to be precursors of squamous cell carcinoma (SCC) of the skin. These lesions can serve as an excellent model system for studying genetic changes associated with the inception of skin SCC. In the present study, five such lesions of the skin, three AKs and two AK+CIS, from three patients were short-term cultured and analyzed cytogenetically. One of the patients (case 3) had also an SCC in addition to three premalignant lesions. All lesions, but one, showed clonal karyotypic abnormalities. The recurrent changes identified were numerical, that is, +7 and +20. The structural rearrangements found in three AK were different, but it could be noted that the distal part of the long arm of chromosome 4 was involved in two AK and the SCC of case 3A. It was also interesting that chromosome 1 participated in structural rearrangements in three AK with band 1p31 being involved in two tumors. The karyotypic profile of these lesions is compared with that of skin SCC; it turns out that the general patterns are different in the sense that the SCC more often have complex karyotypes and display unbalanced aberrations involving the centromeric regions. Some karyotypic similarities between the SCC and their precursors are revealed. The fact that the structural rearrangements involving chromosomal band 3p13 and the centromeric region of chromosome 3 in AK are common features for many types of malignant tumors, including skin SCC, indicates that these changes are early genetic events associated with malignant transformation.
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| 5. |
- Mzezewa, S, et al.
(författare)
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A prospective double blind randomized study comparing the need for blood transfusion with terlipressin or a placebo during early excision and grafting of burns
- 2004
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Ingår i: Burns. - : Elsevier BV. - 0305-4179. ; 30:3, s. 236-240
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: Early excision and skin grafting has become the standard of good burn management, but it is associated with major blood loss. Aim: To determine the haemostatic effect of terlipressin compared with placebo. Material and methods: Fifty-one patients with bums of 10-20% total body surface area had early excision and split skin grafting of deep burns. The surface area of the bum wound and of the heated graft were measured by planimetry. The patients were randomly allocated to medication, either terlipressin or placebo. Blood loss and number of transfused units of blood were recorded. Results: Twenty-one patients received terlipressin, 13 received terlipressin late (cross-over) and 17 received placebo. Six out of 21 patients exposed to terlipressin were transfused with eleven units of packed red blood cells. Seven out of 13 patients crossed over from placebo to terlipressin (late terlipressin) were transfused with 17 units of blood. Eight out of 17 patients exposed to the placebo were transfused with 22 units of blood (P < 0.05). Graft healing was 1055 +/- 609 cm(2) out of 1452 +/- 1811 cm(2) in terlipressin and 914 +/- 633 cm(2) out of 1288 720 cm 2 in the placebo group (n.s.). Conclusion: Terlipressin reduced the need for blood transfusion by a factor of 2.5 compared to a placebo without impairment of graft healing.
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| 6. |
- Mzezewa, S, et al.
(författare)
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Comparison of Surface Swab Cultures and Quantitative Tissue Biopsy Cultures to Predict Sepsis in Burn Patients: A Prospective Study.
- 2003
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Ingår i: Journal of Burn Care and Rehabilitation. - 1534-5939. ; 24:6, s. 365-370
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Tidskriftsartikel (refereegranskat)abstract
- This study aimed at evaluating the possibility of predicting septicemia in burn patients by using wound surface and tissue culture techniques as well as blood cultures. Fifty patients with full-thickness burn wounds covering at least 10% of the total body surface area were included. Signs of septicemia were noted in 21 patients (42%) and 29 patients died (58%). The bacterial colonization of the burn wounds consisted mainly of Staphylococcus aureus and Pseudomonas aeruginosa. Sepsis was better correlated to quantitative burn tissue biopsy cultures than surface swab cultures but the time needed for processing limits its predictive and therapeutic value.
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| 7. |
- Nordquist, J, et al.
(författare)
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Does ultrasound influence experimentally induced thrombus formation in the central artery of the rabbit ear?
- 2000
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Ingår i: Journal of Thrombosis and Thrombolysis. - 1573-742X. ; 9:3, s. 243-249
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Thrombosis is one of the most important causes of morbidity in the medical field. Several independent in vitro studies have shown that the fibrinolytic process may be enhanced by ultrasound, but the effect of ultrasound on thrombus formation in vivo is unexplored. The present study was designed to investigate this matter. METHODS: In a blind randomized study, standardized arteriotomies and intimectomies were performed on the central arteries of the ears of 25 rabbits. The rabbits were allocated to two groups, an untreated control group and a group treated with ultrasound (10 pulses of frequency 1 MHz and intensity 1 W/cm(2) per millisecond giving an averaged intensity of 0.01 W/cm(2)). Immediately after reperfusion, patency was confirmed by a manual empty/refill test, after which blood-flow was monitored using ultrasonic flow-probes twice a minute for two hours. At two hours, patency was rechecked. RESULTS: All vessels were patent at reperfusion, but only seven vessels (three control, four treated) were patent when flow-rate measurements started. At 2 h, patency-frequencies were 12/23 in the control group and 11/22 in the treated group. Flow-rate curves in patent vessels in both groups were similar. Microscopic investigation at one week showed no difference in thrombus accumulation. CONCLUSIONS: Ultrasound with the above characteristics does not significantly improve patency in vivo.
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| 8. |
- Sjoberg, T, et al.
(författare)
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Immune response in burn patients in relation to HIV infection and sepsis
- 2004
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Ingår i: Burns. - : Elsevier BV. - 0305-4179. ; 30:7, s. 670-674
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Tidskriftsartikel (refereegranskat)abstract
- The post-burn immune dysfunction predisposes patients to sepsis and multiple organ failure leading to increased mortality. HIV infection also results in a depressed immune response. The combination of burn injury and HIV might therefore lead to an increased morbidity and mortality as compared to non-HIV infected burn patients. Twenty burn patients and 10 healthy volunteers were included in a prospective study. To evaluate their immune status, CD4+ and CD8+ T-lymphocyte counts were determined in peripheral blood. HIV serology samples were obtained on admission. Bacteriological cultures were obtained from wound surface samples and wound tissue biopsies. Six burn patients were HIV infected. Clinical signs of sepsis were observed in 10 patients. The number of CD4+ T-lymphocytes were lower in burn patients compared to healthy volunteers (P < 0.05). HIV infected burn patients had lower CD4+ lymphocyte counts than non-HIV infected patients (P < 0.05). Patients with clinical signs of sepsis had lower CD4+ counts compared to patients without sepsis (P < 0.05). There was no difference in the mortality rate or the length of hospitalisation between patient groups. burn injury, HIV infection and sepsis independently result in immunosuppression. (C) 2004 Elsevier Ltd and ISBI. All rights reserved.
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