| 1. |
- Kokaia, Z, et al.
(författare)
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BDNF makes cultured dentate granule cells more resistant to hypoglycaemic damage
- 1994
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Ingår i: NeuroReport. - : Ovid Technologies (Wolters Kluwer Health). - 0959-4965 .- 1473-558X. ; 5:10, s. 4-1241
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to explore whether brain-derived neurotrophic factor (BDNF) can improve neuronal survival in cell cultures of rat dentate gyrus subjected to a hypoglycaemic insult. Glucose deprivation for 15 h caused severe neuronal loss (about 70%). BDNF added either 24 h before or 4 h after onset of hypoglycaemia completely protected granule cells against this insult-induced damage. Nerve growth factor (NGF) had similar effects. These findings support the hypothesis that the rapid upregulation of BDNF mRNA in dentate granule cells after brief periods of hypoglycaemic coma and other insults is a local protective mechanism.
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| 2. |
- Nikkhah, G, et al.
(författare)
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Platelet-derived growth factor promotes survival of rat and human mesencephalic dopaminergic neurons in culture
- 1993
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Ingår i: Experimental Brain Research. - 0014-4819 .- 1432-1106. ; 92:3, s. 516-523
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Tidskriftsartikel (refereegranskat)abstract
- The effect of two isoforms of platelet-derived growth factor (PDGF), PDGF-AA and PDGF-BB, was tested on dissociated cell cultures of ventral mesencephalon from rat and human embryos. PDGF-BB but not PDGF-AA reduced the progressive loss of tyrosine hydroxylase- (TH)-positive neurons in rat and human cell cultures. The mean number of TH-positive cells in the PDGF-BB-treated rat culture was 64% and 106% higher than in the control cultures after 7 and 10 days in vitro, respectively. Corresponding figures for human TH-positive neurons were 90% and 145%. The influence of PDGF-BB was specific for TH-positive neurons and not a general trophic effect, since no change of either total cell number or metabolic activity was found. In PDGF-BB-treated cultures of human but not rat tissue the TH-positive neurons had longer neurites than observed in control or PDGF-AA-treated cultures. These data indicate that PDGF-BB may act as a trophic factor for mesencephalic dopaminergic neurons and suggest that administration of PDGF-BB could ameliorate degeneration and possibly promote axonal sprouting of these neurons in vivo.
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| 3. |
- SanMartin, Agneta, et al.
(författare)
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Microarray Analysis Reveals Moderate Gene Expression Changes in Cortical Neural Stem Cells Cultured on Nanowire Arrays
- 2014
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Ingår i: Journal of Nanoscience and Nanotechnology. - : American Scientific Publishers. - 1533-4880 .- 1533-4899. ; 14:7, s. 4880-4885
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Tidskriftsartikel (refereegranskat)abstract
- Although nanowires are widely used in biological applications, especially as cell-manipulation tools, the effect of nanowires on living cells has not been fully investigated. Here, we examined the full gene expression of mouse cortical stem cells cultured on vertical nanowire substrates using RNA microarrays. Genes involved in cell adhesion, cell morphology regulation and cell metabolism were up regulated on the nanowire substrates as compared to flat controls. Scanning electron microscopy images of the samples corroborated our findings, revealing significant differences in cell morphology and adhesion between the nanowire and the flat substrates. Cells on nanowires exhibited a round morphology with a mature appearance characterized by numerous and long processes adhering to the nanowires, while cells on flat substrates had a flat, spread-out morphology and very little neurite outgrowth.
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| 4. |
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| 5. |
- Studer, Lorenz, et al.
(författare)
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Effects of brain-derived neurotrophic factor on neuronal structure of dopaminergic neurons in dissociated cultures of human fetal mesencephalon
- 1996
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Ingår i: Experimental Brain Research. - 0014-4819. ; 108:2, s. 328-336
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Tidskriftsartikel (refereegranskat)abstract
- Brain-derived neurotrophic factor (BDNF) has been shown to promote the survival of cultured fetal mesencephalic dopaminergic neurons of rat and human origin. In the present study, BDNF was tested for its ability to influence neuronal structure of dopaminergic neurons in dissociated cultures of human fetal ventral mesencephalon after 7 days in vitro. Following immunocytochemical staining for tyrosine hydroxylase, all surviving dopaminergic neurons were counted. Computer-assisted three-dimensional reconstructions of uniform randomly selected neurons cultured with 50 ng/ml BDNF (n = 120) or without BDNF (n = 80) were made. BDNF increased the number of surviving human dopaminergic neurons by 76%. Mean soma profile area was significantly enlarged by 18% in BDNF-treated neurons as compared to controls. Analysis of parameters of neuritic size and complexity in these cultures revealed that combined neuritic length, combined neuritic volume, and neuritic field area were increased by 60%, 125% and 129%, respectively, and the mean number of segments per cell was increased by 41%. A change in neurite complexity in BDNF-treated cultures was further confirmed by the Sholl's concentric sphere analysis. These results demonstrate that BDNF promotes development and differentiation of human fetal dopaminergic neurons in vitro.
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