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- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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- Bhiwapurkar, M. K., et al.
(författare)
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Effect of magnitudes and directions (mono-axis and multi-axis) of whole body-vibration exposures and subjects postures on the sketching performance
- 2011
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Ingår i: Proceedings of the Institution of mechanical engineers. Part F, journal of rail and rapid transit. - 0954-4097 .- 2041-3017. ; 225:F1, s. 71-83
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Tidskriftsartikel (refereegranskat)abstract
- Whole-body vibrations in trains are known to affect the performance of sedentary activities such as reading, writing, sketching, working on a computer, etc. The objective of the study was to investigate the extent of disturbance perceived in sketching task by seated subjects in two postures under mono-and multi-axis Gaussian random vibration environment. The study involved 21 healthy male subjects in the age group of 23-32 years. Random vibrations were generated both in mono-and multi-axial directions in the frequency range of 1-10 Hz at 0.5, 1.0, and 1.5 m/s(2) rms (root mean square) amplitude. The subjects were required to sketch given geometric figures such as a circle, triangle, rectangle, and square with the help of ball-point pen under given vibration stimuli in two postures (sketch pad on lap and sketch pad on table). The deviation in distortion with respect to the given figure is represented in terms of percentage distortion. The influence of vibrations on the sketching activity was investigated both subjectively and by two specifically designed objective methods, namely, RMS (root mean square methodology) and area methods. The judgements of perceived difficulty to sketch were rated using seven-point semantic judgement scale. The percentage distortion and difficulty in sketching activity increased with an increase in vibration magnitude. Both subjective evaluation and the RMS method revealed that the task was affected more while sketching on the table. The percentage distortion was affected similarly and maximum in all the vibration directions except for the vertical, while sketching difficulty was found to be higher only with longitudinal and multi-axis vibration direction. The subjective evaluation also revealed that there was no effect of the type of entity chosen on the sketching difficulty.
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- Bhiwapurkar, M. K., et al.
(författare)
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Influence of Mono-axis Random Vibration on Reading Activity
- 2010
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Ingår i: Industrial Health. - 0019-8366 .- 1880-8026. ; 48:5, s. 675-681
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Tidskriftsartikel (refereegranskat)abstract
- Recent studies on train passengers' activities found that many passengers were engaged in some form of work, e.g., reading and writing, while traveling by train. A majority of the passengers reported that their activities were disturbed by vibrations or motions during traveling. A laboratory study was therefore set up to study how low-frequency random vibrations influence the difficulty to read. The study involved 18 healthy male subjects of 23 to 32 yr of age group. Random vibrations were applied in the frequency range (1-10 Hz) at 0.5, 1.0 and 1.5 m/s(2) rms amplitude along three directions (longitudinal, lateral and vertical). The effect of vibration on reading activity was investigated by giving a word chain in two different font types (Times New Roman and Anal) and three different sizes (10, 12 and 14 points) of font for each type. Subjects performed reading tasks under two sitting positions (with backrest support and leaning over a table). The judgments of perceived difficulty to read were rated using 7-point discomfort judging scale. The result shows that reading difficulty increases with increasing vibration magnitudes and found to be maximum in longitudinal direction, but with leaning over a table position. In comparison with Times New Roman type and sizes of font, subjects perceived less difficulty with Anal type for all font sizes under all vibration magnitude.
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