| 1. |
- Magni, Stefano, et al.
(författare)
-
Inferring upstream regulatory genes of FOXP3 in human regulatory T cells from time-series transcriptomic data
- 2024
-
Ingår i: npj Systems Biology and Applications. - : Springer Nature. - 2056-7189. ; 10:1
-
Tidskriftsartikel (refereegranskat)abstract
- The discovery of upstream regulatory genes of a gene of interest still remains challenging. Here we applied a scalable computational method to unbiasedly predict candidate regulatory genes of critical transcription factors by searching the whole genome. We illustrated our approach with a case study on the master regulator FOXP3 of human primary regulatory T cells (Tregs). While target genes of FOXP3 have been identified, its upstream regulatory machinery still remains elusive. Our methodology selected five top-ranked candidates that were tested via proof-of-concept experiments. Following knockdown, three out of five candidates showed significant effects on the mRNA expression of FOXP3 across multiple donors. This provides insights into the regulatory mechanisms modulating FOXP3 transcriptional expression in Tregs. Overall, at the genome level this represents a high level of accuracy in predicting upstream regulatory genes of key genes of interest.
|
|
| 2. |
- Sawlekar, Rucha, et al.
(författare)
-
A Survey of DNA-based Computing Devices and their Applications
- 2021
-
Ingår i: Proceedings of the 2021 European Control Conference (ECC). - : IEEE. ; , s. 769-774
-
Konferensbidrag (refereegranskat)abstract
- The research on DNA-based circuits has grown exponentially during the past few years. Having said that, the technology needed for the implementation of such circuits in living cells has been upgraded immensely as well. DNA exhibits the properties of programmable structure, self-assembly and adequate robust stability, making it an important and interesting building block to construct molecular machines. In the attempt to provide an alternative to the traditional semiconductor-based devices, extensive research has been done to construct molecular level devices with minimal components performing efficiently in living cells. In this article, we present a comprehensive survey of advancements and applications of such devices in molecular computing and nanotechnology. We cover the overview of programming languages, design and development of computational devices like logic gates and controllers, including the former approaches that originally influenced this present development. We also discuss nanorobots, such as DNA walkers and tweezers with the general objective to provide a comprehensive survey of the most state of the art approaches in the DNA based computing devices.
|
|
| 3. |
|
|
| 4. |
- V. Saucedo, Mario A., et al.
(författare)
-
Event Camera and LiDAR based Human Tracking for Adverse Lighting Conditions in Subterranean Environments
- 2023
-
Ingår i: 22nd IFAC World Congress. - : Elsevier. ; , s. 9257-9262
-
Konferensbidrag (refereegranskat)abstract
- In this article, we propose a novel LiDAR and event camera fusion modality for subterranean (SubT) environments for fast and precise object and human detection in a wide variety of adverse lighting conditions, such as low or no light, high-contrast zones and in the presence of blinding light sources. In the proposed approach, information from the event camera and LiDAR are fused to localize a human or an object-of-interest in a robot's local frame. The local detection is then transformed into the inertial frame and used to set references for a Nonlinear Model Predictive Controller (NMPC) for reactive tracking of humans or objects in SubT environments. The proposed novel fusion uses intensity filtering and K-means clustering on the LiDAR point cloud and frequency filtering and connectivity clustering on the events induced in an event camera by the returning LiDAR beams. The centroids of the clusters in the event camera and LiDAR streams are then paired to localize reflective markers present on safety vests and signs in SubT environments. The efficacy of the proposed scheme has been experimentally validated in a real SubT environment (a mine) with a Pioneer 3AT mobile robot. The experimental results show real-time performance for human detection and the NMPC-based controller allows for reactive tracking of a human or object of interest, even in complete darkness.
|
|
