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| 2. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 3. |
- Muller, S, et al.
(författare)
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Target 2035 - update on the quest for a probe for every protein
- 2022
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Ingår i: RSC medicinal chemistry. - : Royal Society of Chemistry (RSC). - 2632-8682. ; 13:1, s. 13-21
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Twenty years after the publication of the first draft of the human genome, our knowledge of the human proteome is still fragmented. Target 2035 aims to develop a pharmacological modulator for every protein in the human proteome to fill this gap.
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| 4. |
- Ackloo, S, et al.
(författare)
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Target 2035 - an update on private sector contributions
- 2023
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Ingår i: RSC medicinal chemistry. - : Royal Society of Chemistry (RSC). - 2632-8682. ; 14:6, s. 1002-1011
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Target 2035, an international federation of biomedical scientists from the public and private sectors, is leveraging ‘open’ principles to develop a pharmacological tool for every human protein.
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| 5. |
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| 6. |
- Arrowsmith, CH, et al.
(författare)
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The promise and peril of chemical probes
- 2015
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Ingår i: Nature chemical biology. - : Springer Science and Business Media LLC. - 1552-4469 .- 1552-4450. ; 11:8, s. 536-541
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Tidskriftsartikel (refereegranskat)
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| 7. |
- Chiesa, Marco, 1987-, et al.
(författare)
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SIXPACK : Securing internet eXchange points against curious onlookers
- 2017
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Ingår i: CoNEXT 2017 - Proceedings of the 2017 13th International Conference on emerging Networking EXperiments and Technologies. - New York, NY, USA : Association for Computing Machinery (ACM). - 9781450354226 ; , s. 120-133
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Konferensbidrag (refereegranskat)abstract
- Internet eXchange Points (IXPs) play an ever-growing role in Internet inter-connection. To facilitate the exchange of routes amongst their members, IXPs provide Route Server (RS) services to dispatch the routes according to each member's peering policies. Nowadays, to make use of RSes, these policies must be disclosed to the IXP. This poses fundamental questions regarding the privacy guarantees of route-computation on confidential business information. Indeed, as evidenced by interaction with IXP administrators and a survey of network operators, this state of affairs raises privacy concerns among network administrators and even deters some networks from subscribing to RS services. We design sixpack1, an RS service that leverages Secure Multi-Party Computation (SMPC) to keep peering policies confidential, while extending, the functionalities of today's RSes. As SMPC is notoriously heavy in terms of communication and computation, our design and implementation of sixpack aims at moving computation outside of the SMPC without compromising the privacy guarantees. We assess the effectiveness and scalability of our system by evaluating a prototype implementation using traces of data from one of the largest IXPs in the world. Our evaluation results indicate that sixpack can scale to support privacy-preserving route-computation, even at IXPs with many hundreds of member networks.
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| 8. |
- Chiesa, Marco, 1987-, et al.
(författare)
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Towards securing internet eXchange points against curious onlooKers
- 2016
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Ingår i: ANRW 2016 - Proceedings of the ACM, IRTF and ISOC Applied Networking Research Workshop. - New York, NY, USA : Association for Computing Machinery (ACM). - 9781450344432 ; , s. 32-34
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Konferensbidrag (refereegranskat)abstract
- The growing relevance of Internet eXchange Points (IXPs), where an increasing number of networks exchange routing information, poses fundamental questions regarding the privacy guarantees of confidential business information. To facilitate the exchange of routes among their members, IXPs provide Route Server (RS) services to dispatch the routes according to each member's export policies. Nowadays, to make use of RSes, these policies must be disclosed to the IXP. This state of affairs raises privacy concerns among network administrators and even deters some networks from subscribing to RS services. We design SIXPACK (which stands for "Securing Internet eXchange Points Against Curious onlooKers"), a RS service that leverages Secure Multi-Party Computation (SMPC) techniques to keep export policies confidential, while maintaining the same functionalities as today's RSes. We assess the effectiveness and scalability of our system by evaluating our prototype implementation and using traces of data from one of the largest IXPs in the world.
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| 9. |
- Dittrich, Christian, et al.
(författare)
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ESMO / ASCO Recommendations for a Global Curriculum in Medical Oncology Edition 2016
- 2016
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Ingår i: ESMO Open. - : Elsevier BV. - 2059-7029. ; 1:5
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Tidskriftsartikel (refereegranskat)abstract
- The European Society for Medical Oncology (ESMO) and the American Society of Clinical Oncology (ASCO) are publishing a new edition of the ESMO/ ASCO Global Curriculum (GC) thanks to contribution of 64 ESMOappointed and 32 ASCO-appointed authors. First published in 2004 and updated in 2010, the GC edition 2016 answers to the need for updated recommendations for the training of physicians in medical oncology by defining the standard to be fulfilled to qualify as medical oncologists. At times of internationalisation of healthcare and increased mobility of patients and physicians, the GC aims to provide state-of-the-art cancer care to all patients wherever they live. Recent progress in the field of cancer research has indeed resulted in diagnostic and therapeutic innovations such as targeted therapies as a standard therapeutic approach or personalised cancer medicine specialised training for medical oncology trainees. Thus, several new chapters on technical contents such as molecular pathology, translational research or molecular imaging and on conceptual attitudes towards human principles like genetic counselling or survivorship have been integrated in the GC. The GC edition 2016 consists of 12 sections with 17 subsections, 44 chapters and 35 subchapters, respectively. Besides renewal in its contents, the GC underwent a principal formal change taking into consideration modern didactic principles. It is presented in a template-based format that subcategorises the detailed outcome requirements into learning objectives, awareness, knowledge and skills. Consecutive steps will be those of harmonising and implementing teaching and assessment strategies.
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