| 1. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 2. |
- Bountra, K., et al.
(författare)
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Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD
- 2017
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Ingår i: Embo Journal. - : EMBO. - 0261-4189 .- 1460-2075. ; 36:20, s. 3062-3079
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Tidskriftsartikel (refereegranskat)abstract
- Certain pathogenic bacteria produce and release toxic peptides to ensure either nutrient availability or evasion from the immune system. These peptides are also toxic to the producing bacteria that utilize dedicated ABC transporters to provide self-immunity. The ABC transporter McjD exports the antibacterial peptide MccJ25 in Escherichia coli. Our previously determined McjD structure provided some mechanistic insights into antibacterial peptide efflux. In this study, we have determined its structure in a novel conformation, apo inward-occluded and a new nucleotide-bound state, high-energy outward-occluded intermediate state, with a defined ligand binding cavity. Predictive cysteine cross-linking in E.coli membranes and PELDOR measurements along the transport cycle indicate that McjD does not undergo major conformational changes as previously proposed for multi-drug ABC exporters. Combined with transport assays and molecular dynamics simulations, we propose a novel mechanism for toxic peptide ABC exporters that only requires the transient opening of the cavity for release of the peptide. We propose that shielding of the cavity ensures that the transporter is available to export the newly synthesized peptides, preventing toxic-level build-up.
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| 3. |
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| 4. |
- Schiemann, M., et al.
(författare)
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Technical solutions to foster the global energy transition: Special issue on clean fuel conversion technologies for carbon dioxide and pollutant reduction
- 2022
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Ingår i: Renewable & sustainable energy reviews. - : Elsevier. - 1364-0321 .- 1879-0690. ; 154
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Tidskriftsartikel (refereegranskat)abstract
- In the field of energy supply, the focus is shifting from conventional combustion to a variety of new energy conversion technologies. Nineteen articles in this Special Issue describe different aspects, that make combustion and combustion products attractive in the context of future sustainable energy systems. Carbon capture and recycling can bridge the current energy system and the transition to 100% renewables. The energy storage required for this is possible with combustion systems and specific synthetic fuels. In this context, bio-based fuel applications also benefit from current research and support flexible and efficient reduction of carbon dioxide emissions. All these aspects call for better experimental and numerical methods for process characterization and design.
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