| 1. |
- Murphy, M. P., et al.
(författare)
-
Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo
- 2022
-
Ingår i: Nature Metabolism. - : Springer Science and Business Media LLC. - 2522-5812. ; 4:6, s. 651-662
-
Tidskriftsartikel (refereegranskat)abstract
- Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo. Reactive oxygen species (ROS) have important roles in health and disease, but are chemically complex and difficult to measure accurately. This consensus statement proposes guidelines and best practices on the nomenclature and assessment of ROS, oxidative reactions and oxidative damage in cells, tissues and in vivo.
|
|
| 2. |
- Laurent, C M, et al.
(författare)
-
Stability of RPE increase during repeated intermittent sprints
- 2010
-
Ingår i: Journal of exercise science & fitness. - 1728-869X. ; 8:1, s. 1-10
-
Tidskriftsartikel (refereegranskat)abstract
- The current investigation examined the potential teleoanticipatory effect on perceptual response during repeated bouts of maximal sprint work. To determine the consistency of ratings of perceived exertion (RPE) increase during identical exercise bouts following variable recovery periods, 16 (8 men, 8 women) participants completed four separate trials of repeated maximal sprinting on 4 separate days utilizing different recovery periods. Following completion of the baseline trial, participants were given variable, counter-balanced recovery periods of 24, 48 and 72 hours, whereupon they repeated the intermittent exercise protocol. To determine the degree of similarity among trials, each individual's rate of RPE progression during each cycle of eight sprints throughout the recovery trials were compared to the rate of progression during the baseline exercise session. A series of 4 (trial) x 3 (cycle of sprints) repeated measures ANOVA were performed to identify significant main effects between trials and among cycles while session RPE was analyzed using one-way repeated measures ANOVA. Fisher's least significant difference post-hoc procedures were performed to identify where significant differences occurred when appropriate. Results revealed an inconsistency in the stability of RPE across repeated bouts of sprint exercise, with at least 50% of individuals having a substantial difference in RPE (i.e. +/- 1 unit change) in at least one subsequent trial. These variations in perceptual responses were observed despite a concomitant stability of physiological and performance responses between sessions. Results suggest that rate of RPE increase correspond more closely to increased or decreased physiologic strain than to an anticipatory, feed-forward mechanism following variable recovery durations.
|
|
