| 1. |
- Kozhevnikov, Evgeny, et al.
(författare)
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A dual-transduction-integrated biosensing system to examine the 3D cell-culture for bone regeneration
- 2019
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Ingår i: Biosensors & bioelectronics. - : ELSEVIER ADVANCED TECHNOLOGY. - 0956-5663 .- 1873-4235. ; 141
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Tidskriftsartikel (refereegranskat)abstract
- Three-dimensional (3D) cell cultures developed with living cells and scaffolds have demonstrated outstanding potential for tissue engineering and regenerative medicine applications. However, no suitable tools are available to monitor dynamically variable cell behavior in such a complex microenvironment. In particular, simultaneously assessing cell behavior, cell secretion, and the general state of a 3D culture system is of a really challenging task. This paper presents our development of a dual-transduction-integrated biosensing system that assesses electrical impedance in conjunction with imaging techniques to simultaneously investigate the 3D cell-culture for bone regeneration. First, we created models to mimic the dynamic deposition of the extracellular matrix (ECM) in 3D culture, which underwent osteogenesis by incorporating different amounts of bone-ECM components (collagen, hydroxyapatite [HAp], and hyaluronic acid [HA]) into alginate-based hydrogels. The formed models were investigated by means of electrical impedance spectroscopy (EIS), with the results showing that the impedances increased linearly with collagen and hyaluronan, but changed in a more complex manner with HAp. Thereafter, we created two models that consisted of primary osteoblast cells (OBs), which expressed the enhanced green fluorescent protein (EGFP), and 4T1 cells, which secreted the EGFP-HA, in the alginate hydrogel. We found the capacitance (associated with impedance and measured by EIS) increased with the increases in initial embedded OBs, and also confirmed the cell proliferation over 3 days with the EGFP signal as monitored by the fluorescent imaging component in our system. Interestingly, the change in capacitance is found to be associated with OB migration following stimulation. Also, we show higher capacitance in 4T1 cells that secret HA when compared to control 4T1 cells after a 3-day culture. Taken together, we demonstrate that our biosensing system is able to investigate the dynamic process of 3D culture in a non-invasive and real-time manner.
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| 2. |
- Wang, Lihui, et al.
(författare)
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Development of a Function Block Designer for Collaborative Process Planning
- 2005
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Ingår i: Proceedings of the 9th International Conference on Computer Supported Cooperative Work in Design. - : Institute of Electrical and Electronics Engineers (IEEE). - 1846000025 ; , s. 217-222
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Konferensbidrag (refereegranskat)abstract
- The research objective is to develop methodologies and framework for collaborative process planning and scheduling, supported by a real-time monitoring system in distributed environments. A function block enabled collaborative process planning approach is proposed to handle various dynamic changes during process plan generation and execution. This paper focuses on collaborative process planning, particularly on the development of a function block designer As function blocks can sense environmental changes, it is expected that a so generated process plan can adapt itself to the changes with dynamically optimized solutions for plan execution and process monitoring.
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| 3. |
- Wang, Lihui, et al.
(författare)
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FBD : A Function Block Designer for Distributed and Collaborative Process Planning
- 2006
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Ingår i: Computer Supported Cooperative Work in Design II. - : Springer Berlin/Heidelberg. - 3540329692 ; , s. 434-444
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Bokkapitel (refereegranskat)abstract
- The objective of this research is to develop methodologies and framework for distributed and collaborative process planning. Facilitated by a real-time monitoring system, the proposed methodologies can also be applied to integrate with functions of dynamic scheduling in a distributed environment. A function block enabled collaborative process planning approach is proposed to handle dynamic changes during process plan generation and execution. This chapter focuses on collaborative process planning, particularly on the development of a function block designer. As function blocks can sense environmental changes in a shop floor, it is expected that a so generated process plan can adapt itself to the shop floor environment with dynamically optimized solutions for plan execution and process monitoring.
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| 4. |
- Zhu, Yanji, et al.
(författare)
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Nano SiC enhancement in the BN micro structure for high thermal conductivity epoxy composite
- 2021
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Ingår i: Journal of polymer research. - : Springer. - 1022-9760 .- 1572-8935. ; 28:10
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Tidskriftsartikel (refereegranskat)abstract
- Improving the heat dissipation efficiency of electronic products is the key to the design of many modern electronic and mechanical systems. Herein, we combined the 3D network fabricating with the way of micron-nano reinforcement to prepare high thermal conductivity and excellent thermal stability composites. Epoxy resin was used as the matrix, while the silicon carbide foam (f-SiC) as skeleton and the BN/nano-SiC as thermally conductive fillers. The thermal conductivity of the EP/f-SiC/BN/nano-SiC composite reaches 3.5 W·m− 1·K− 1, which is about 16.6 times higher than that of pure epoxy resin. The characterization results of TC and infrared thermography images indicate that the EP/f-SiC/BN/nano-SiC composite possess superior heat transport performance. Meantime, the EP/f-SiC/BN/nano-SiC composite have excellent thermal stability, the THRI of EP/f-SiC/BN/nano-SiC reaches 195.8℃, which is 21.3℃ higher than that of pure EP. This work would provide a new strategy for improving the TC of polymers by using other 3D skeletons and micron-nano fillers, and is conducive to the development of high thermal conductivity and excellent thermal stability materials.
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