| 1. |
|
|
| 2. |
|
|
| 3. |
- Guevara-Martínez, Mónica, 1989-, et al.
(författare)
-
Regulating the production of (R)-3-hydroxybutyrate in Escherichia coli by N or P limitation
- 2015
-
Ingår i: Frontiers in Microbiology. - : Frontiers Research Foundation. - 1664-302X. ; 6
-
Tidskriftsartikel (refereegranskat)abstract
- The chiral compound (R)-3-hydroxybutyrate (3HB) is naturally produced by many wild type organisms as the monomer for polyhydroxybutyrate (PHB). Both compounds are commercially valuable and co-polymeric polyhydroxyalkanoates have been used e.g., in medical applications for skin grafting and as components in pharmaceuticals. In this paper we investigate cultivation strategies for production of 3HB in the previously described E. coil strain AF1000 pJBGT3RX. This strain produces extracellular 3HB by expression of two genes from the PHB pathway of Halomonas boliviensis. H. boliviensis is a newly isolated halophile that forms PHB as a storage compound during carbon excess and simultaneous limitation of another nutrient like nitrogen and phosphorous. We hypothesize that a similar approach can be used to control the flux from acetylCoA to 3HB also in E coli; decreasing the flux to biomass and favoring the pathway to the product. We employed ammonium- or phosphate-limited fed-batch processes for comparison of the productivity at different nutrient limitation or starvation conditions. The feed rate was shown to affect the rate of glucose consumption, respiration, 3HB, and acetic acid production, although the proportions between them were more difficult to affect. The highest 3HB volumetric productivity, 1.5 g L-1 h(-1), was seen for phosphate-limitation.
|
|
| 4. |
- Lundberg, Olle, et al.
(författare)
-
The effect of social protection and income maintenance policies on health and health inequalities
- 2013
-
Ingår i: European Journal of Public Health. - : Oxford University Press (OUP). - 1101-1262 .- 1464-360X. ; 23:Suppl. 1
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- BackgroundAs a starting point we review the existing evidence on welfare states, health and health inequalities, from 2005 onwards. Three different approaches emerge in these previous studies – the welfare regime approach, the welfare institutions approach and the social spending approach. While no clear picture emerges for the welfare regime approach, summarising findings regarding the institutional and expenditure approach suggest that a higher degree of generosity and social spending benefits public health. These are therefore approaches we follow to arrive at a better understanding of what type of policies are linked to smaller inequalities in health across the life-course.MethodsA starting point in the analyses is the relation between income, poverty and mortality. The cross-national variation in poverty rates, both absolute (poverty threshold) and relative (60 per cent of median income) measure, and mortality rates in European 26 countries will be considered.The second step in the analysis focuses on the relationship between social rights and subjective health in Europe, with a focus on national variations and changes in social rights to levels and changes in subjective health outcomes across several countries. The data holds information regarding social rights and social expenditure, including individual data from EU-SILC.ResultsPreliminary results indicate that it is the totality of social protection that is important rather than individual policies. A sub-study regarding social rights and health among youth highlight also the importance of active and passive labour market policy in the 16 included countries.ConclusionsIn sum our diverse approach to analysing welfare state efforts and their links to health inequalities suggest that there is a clear relationship between more ambitious policies and smaller inequalities in health. These results are discussed in relation to previous findings.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
- Palmberg, Lars Johan, 1713-1804
(författare)
-
Dissertatio academica, de antiquitatum scientia, cujus partem quartam, adprobante ampliss. facultate philosophica, in regia academia Upsaliensi, publice bonorum censuræ committunt auctor, mag. Laur. J. Palmberg, ... respondens ..., Magnus Sjöberg, Roslagus. In auditorio Gustav. majori die XX. decemb. MDCCLXXIX. ..., P. 4
- 1779
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)
|
|
| 8. |
- Pöysä, Hannu, et al.
(författare)
-
Recovering Whooper Swans do not cause a decline in Eurasian Wigeon via their grazing impact on habitat
- 2018
-
Ingår i: Journal of Ornithology = Journal fur Ornithologie. - : Springer Science Business Media. - 0021-8375 .- 1439-0361. ; 159, s. 447-455
-
Tidskriftsartikel (refereegranskat)abstract
- The Whooper Swan (Cygnus cygnus) is a good example of successful conservation, with rapidly growing numbers in Fennoscandia in recent decades. To the contrary, Eurasian Wigeon (Mareca penelope) shows a strong negative trend in breeding numbers, which raises conservation concerns. Previous research suggests a causal link between recent population trajectories of the two species. Both preferentially breed on wetlands with abundant horsetail (Equisetum spp.), a plant providing food for Whooper Swan and crucial feeding microhabitat for Eurasian Wigeon broods. We here test predictions based on the hypothesis that grazing on Equisetum by Whooper Swan reduces breeding habitat or breeding habitat quality for Eurasian Wigeon. We use data from 60 lakes in which waterfowl were counted in 1990–1991 and 2016, and Equisetum was mapped in 1990–1991 and 2013–2014. Lakes colonized by Whooper Swan typically had more abundant Equisetum vegetation in the past than lakes not colonized. Lake-specific decrease of Equisetum was not associated with colonization by Whooper Swan. The number of lakes occupied by Eurasian Wigeon decreased, but the decrease was not stronger on lakes colonized by Whooper Swan than on those that were not. Contrary to our prediction, current Eurasian Wigeon abundance was positively associated with Whooper Swan abundance. Moreover, Eurasian Wigeon did not decrease more on lakes from which Equisetum disappeared than on lakes in which there was still Equisetum left. This study does not support the idea that Whooper Swan affects Eurasian Wigeon negatively by grazing on Equisetum.
|
|
| 9. |
- Rein-Hedin, Erik, et al.
(författare)
-
Utilizing venous occlusion plethysmography to assess vascular effects: A study with buloxibutid, an angiotensin II type 2 receptor agonist
- 2024
-
Ingår i: Clinical and Translational Science. - : WILEY. - 1752-8054 .- 1752-8062. ; 17:2
-
Tidskriftsartikel (refereegranskat)abstract
- Buloxibutid (also known as C21) is a potent and selective angiotensin II type 2 receptor (AT2R) agonist, in development for oral treatment of fibrotic lung disease. This phase I, open-label, pharmacodynamic study investigated vascular effects of buloxibutid in five healthy male volunteers. Subjects were administered intra-arterial infusions of buloxibutid for 5 min in ascending doses of 3, 10, 30, 100, and 200 mu g/min, infused sequentially in the forearm. Infusions of sodium nitroprusside (SNP) solution in doses of 0.8-3.2 mu g/min were administered as a positive control. Forearm blood flow (FBF) was measured by venous occlusion plethysmography. Safety and tolerability of intra-arterial administrations of buloxibutid were evaluated. Following infusion of buloxibutid in doses of 3-200 mu g/min, the range of increase in FBF was 27.8%, 17.2%, 37.0%, 28.5%, and 60.5%, compared to the respective baseline. The largest increase was observed in the highest dose group. Infusions of SNP as a positive control, increased FBF 230-320% compared to baseline. Three adverse events (AEs) of mild intensity, not related to buloxibutid or SNP, were reported for two subjects. Two of these AEs were related to study procedures. There were no clinically relevant changes in arterial blood pressure during the study period. Intra-arterial infusion of buloxibutid in low, ascending doses increased FBF, indicating that buloxibutid may be effective in conditions associated with endothelial dysfunction. Venous occlusion plethysmography was found to be a useful method to explore pharmacodynamic vascular effects of novel AT2R agonists, while avoiding systemic adverse effects.
|
|
| 10. |
|
|
