| 1. |
- Ahlbeck Bergendahl, Ida, et al.
(författare)
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Fisk- och skaldjursbestånd i hav och sötvatten 2016 : Resursöversikt
- 2016
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I rapporten kan du ta del av bedömningen som görs av situationen för bestånd som regleras inom ramen för EU:s gemensamma fiskeripolitik (GFP). Bedömningarna baseras på det forskningssamarbete och den rådgivning som sker inom det Internationella Havsforskningsrådet (ICES).De bestånd som förvaltas nationellt baseras på de biologiska underlagen, och rådgivningen i huvudsak på den forskning och övervakning samt analys som bedrivs av Institutionen för akvatiska resurser vid Sveriges lantbruksuniversitet (SLU Aqua) samt yrkesfiskets rapportering.Rapporten omfattar 41 fiskarter uppdelade i olika bestånd, samt sju skal- och blötdjursarter.Nytt för årets upplaga är kapitlet om ekosystemtjänster. Avsnittet beskriver de fördelar människan får genom ekosystemen, till exempel hur fisk och skaldjur kommer till nytta för människan genom föda, rekreation och biologisk mångfald. Nytt för i år är också att rapportens diagram och figurer anpassats för läsare med defekt färgseende.Översikten är utarbetad av SLU Aqua på uppdrag av Havs- och vattenmyndigheten.
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| 2. |
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| 3. |
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| 4. |
- Graae, Lisette, et al.
(författare)
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Self-declarations of environmental classification at Fass.se - Experiences from the reviewing process during 2019
- 2021
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Since 2005 Sweden has a unique environmental classification system for pharmaceutical products. It is a self-declaration system where each pharmaceutical company is responsible for their own environmental information, which is published on the open web-based portal www.Fass.se. Prior to publication the environmental risk assessments are reviewed by IVL Swedish Environmental Research Institute (IVL) as an independent, external part. The present report describes the experiences from the review process during the year 2019. In 2019, environmental risk assessments (ERAs) were sent in for review 421 times. 63% of the reviewed assessments received the comment no remarks and were recommended to be published, whereas the other 37% were recommended to be corrected or needed to be corrected before publication. The number of unique substances that were published at Fass.se during 2019 was 315. Of these substances 43% were exempted from classification, 27% were classified regarding environmental risk, and 30% could not gain any classification due to lack of data. The work of improving the review system is an on-going process. As a part of this work IVL performs studies and activities to increase the knowledge of pharmaceuticals in the environment. In 2018/2019 a model was developed for environmental assessment of pharmaceutical products, in regard to environmental risks related to emissions of Active Pharmaceutical Ingredient (API) from production processes and product carbon footprint in a life cycle perspective.
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| 5. |
- Jansson, Ann, 1950, et al.
(författare)
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Role of a consensus AP-2 regulatory sequence within the Epstein-Barr Virus LMP1 promoter in EBNA2 mediated transactivation
- 2007
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Ingår i: Virus Genes. - : Springer Science and Business Media LLC. - 0920-8569 .- 1572-994X. ; 35:2, s. 203-14
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Tidskriftsartikel (refereegranskat)abstract
- The Epstein-Barr virus (EBV) tumor-associated latent membrane protein 1 (LMP1) gene expression is transactivated by EBV nuclear antigen 2 (EBNA2) in human B cells. We previously reported that an E-box element at the LMP1 regulatory sequence (LRS) represses transcription of the LMP1 gene through the recruitment of a Max-Mad1-mSin3A complex. In the present study, using deletion/mutation analysis, and electrophoretic mobility shift assays, we show that the promoter region adjacent to the E-box (-59/-67) is required for the full repression conferred by E-box binding proteins. The repressive effect of these factors was overcome by an inhibitor of histone deacetylation, Trichostatin A (TSA), concurring with the reports that histone deacetylation plays an important role in repression mediated by Max-Mad1-mSin3A complex. Furthermore, ChIP analyses showed that histones at the transcriptionally active LMP1 promoter were hyperacetylated, whereas in the absence of transcription they were hypoacetylated. EBNA2 activation of the promoter required a consensus AP-2 sequence in the -103/-95 LRS region. While EMSA results and the low level of AP-2 factors expression in B cells argue against known AP-2 factors binding to this site, several pieces of evidence point to a similar mechanism of promoter activation as seen by AP-2 factors. We conclude that an AP-2 site-binding factor and EBNA2 act in concert to overcome the repression of the LMP1 promoter via the consensus AP-2 site. This activation showed strong correlation with histone hyperacetylation at the promoter, indicating this to be a major mechanism for the EBNA2 mediated LMP1 transactivation.
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| 6. |
- Jarvius, Malin, et al.
(författare)
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In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method
- 2007
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 6:9, s. 1500-1509
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Tidskriftsartikel (refereegranskat)abstract
- Improved methods are needed for in situ characterization of post-translational modifications in cell lines and tissues. For example, it is desirable to monitor the phosphorylation status of individual receptor tyrosine kinases in samples from human tumors treated with inhibitors to evaluate therapeutic responses. Unfortunately the leading methods for observing the dynamics of tissue post-translational modifications in situ, immunohistochemistry and immunofluorescence, exhibit limited sensitivity and selectivity. Proximity ligation assay is a novel method that offers improved selectivity through the requirement of dual recognition and increased sensitivity by including DNA amplification as a component of detection of the target molecule. Here we therefore established a generalized in situ proximity ligation assay to investigate phosphorylation of platelet-derived growth factor receptor β (PDGFRβ) in cells stimulated with platelet-derived growth factor BB. Antibodies specific for immunoglobulins from different species, modified by attachment of DNA strands, were used as secondary proximity probes together with a pair of primary antibodies from the corresponding species. Dual recognition of receptors and phosphorylated sites by the primary antibodies in combination with the secondary proximity probes was used to generate circular DNA strands; this was followed by signal amplification by replicating the DNA circles via rolling circle amplification. We detected tyrosine phosphorylated PDGFRβ in human embryonic kidney cells stably overexpressing human influenza hemagglutinin-tagged human PDGFRβ in porcine aortic endothelial cells transfected with the β-receptor, but not in cells transfected with the α-receptor, and also in immortalized human foreskin fibroblasts, BJ hTert, endogenously expressing the PDGFRβ. We furthermore visualized tyrosine phosphorylated PDGFRβ in tissue sections from fresh frozen human scar tissue undergoing wound healing. The method should be of great value to study signal transduction, screen for effects of pharmacological agents, and enhance the diagnostic potential in histopathology.
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| 7. |
- Langer, Sarka, 1960, et al.
(författare)
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Squalene Depletion in Skin Following Human Exposure to Ozone under Controlled Chamber Conditions
- 2024
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Ingår i: Environmental Science & Technology. - 0013-936X .- 1520-5851. ; 58:15, s. 6693-6703
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Tidskriftsartikel (refereegranskat)abstract
- A major component of human skin oil is squalene, a highly unsaturated hydrocarbon that protects the skin from atmospheric oxidants. Skin oil, and thus squalene, is continuously replenished on the skin surface. Squalene is also quickly consumed through reactions with ozone and other oxidants. This study examined the extent of squalene depletion in the skin oils of the forearm of human volunteers after exposure to ozone in a climate chamber. Temperature, relative humidity (RH), skin coverage by clothing, and participants’ age were varied in a controlled manner. Concentrations of squalene were determined in skin wipe samples collected before and after ozone exposure. Exposures to ozone resulted in statistically significant decreases in post-exposure squalene concentrations compared to pre-exposure squalene concentrations in the skin wipes when squalene concentrations were normalized by concentrations of co-occurring cholesterol but not by co-occurring pyroglutamic acid (PGA). The rate of squalene loss due to ozonolysis was lower than its replenishment on the skin surface. Within the ranges examined, temperature and RH did not significantly affect the difference between normalized squalene levels in post-samples versus pre-samples. Although not statistically significant, skin coverage and age of the volunteers (three young adults, three seniors, and three teenagers) did appear to impact squalene depletion on the skin surfaces.
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| 8. |
- Ljungström, Viktor, et al.
(författare)
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Whole-exome sequencing in relapsing chronic lymphocytic leukemia : clinical impact of recurrent RPS15 mutations
- 2016
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Ingår i: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 127:8, s. 1007-1016
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Tidskriftsartikel (refereegranskat)abstract
- Fludarabine, cyclophosphamide and rituximab (FCR) is first-line treatment for medically fit chronic lymphocytic leukemia (CLL) patients, however despite good response rates many patients eventually relapse. Whilst recent high-throughput studies have identified novel recurrent genetic lesions in adverse-prognostic CLL, the mechanisms leading to relapse after FCR therapy are not completely understood. To gain insight into this issue, we performed whole-exome sequencing of sequential samples from 41 CLL patients who were uniformly treated with FCR but relapsed after a median of 2 years. In addition to mutations with known adverse-prognostic impact (TP53, NOTCH1, ATM, SF3B1, NFKBIE, BIRC3) a large proportion of cases (19.5%) harbored mutations in RPS15, a gene encoding a component of the 40S ribosomal subunit. Extended screening, totaling 1119 patients, supported a role for RPS15 mutations in aggressive CLL, with one-third of RPS15-mutant cases also carrying TP53 aberrations. In most cases selection of dominant, relapse-specific subclones was observed over time. However, RPS15 mutations were clonal prior to treatment and remained stable at relapse. Notably, all RPS15 mutations represented somatic missense variants and resided within a 7 amino-acid evolutionarily conserved region. We confirmed the recently postulated direct interaction between RPS15 and MDM2/MDMX and transient expression of mutant RPS15 revealed defective regulation of endogenous p53 compared to wildtype RPS15. In summary, we provide novel insights into the heterogeneous genetic landscape of CLL relapsing after FCR treatment and highlight a novel mechanism underlying clinical aggressiveness involving a mutated ribosomal protein, potentially representing an early genetic lesion in CLL pathobiology.
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| 9. |
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| 10. |
- Potter, Annika, et al.
(författare)
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Results from the Swedish National Screening Programme 2004 : Subreport 3: Limonene
- 2005
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- IVL har på uppdrag av Naturvårdsverket utfört en screening av limonen i Sverige. Limonen är en terpen som finns naturligt i vår omgivning där den avges från växtlighet, t ex barrskogar. Limonen förekommer som två optiska isomerer: d och l. I Sverige är det framför allt l-limonen som emitteras naturligt då den avges bl a från tall. Limonen används som smak- och lukttillsatts till många livsmedel, hushållsprodukter, hygienprodukter och parfymer. Dessutom används limonen som lösningsmedel inom t ex färgindustrin. I troposfären bidrar limonen, tillsammans med andra volatila kolväten, till den fotokemiska bildningen av ozon. Limonen har visats sig vara toxisk för fisk och zooplankton. Flera oxidationsprodukter av limonen kan orsaka skador på vegetation. Den mänskliga hälsan kan påverkas bl a genom att limonen kan orsaka överkänslighet. I screeningstudien ingick provtagning och analys av d- och l-limonen i både luft och vatten från två bakgrundsstationer, den ena placerad vid havet, den andra i ett skogsområde. Sedimentprover från tre bakgrundslokaler i Östersjön har analyserats. Luftprov från en urban mätplats i Göteborg och tre slamprover från reningsverk inkluderades. Två punktkällor, en massaindustri och en juciefabrik har också undersökts. Länsstyrelserna har också haft möjlighet att skicka in prover för analys. Totalt fem länsstyrelser deltog och bidrog med 34 prover varav 19 slamprov, nio vattenprov, två fiskprov och fyra sedimentprov. Resultaten från luftmätningarna visade inte på några ökade limonenkoncentrationer i Göteborg jämfört med bakgrundsstationerna. Inte heller fördelningen mellan d- och l-limonen visade på någon skillnad i ursprunget av limonen i staden jämfört med bakgrundsstationerna. Halterna av främst d-limonen, ökade med stigande omgivningstemperatur i skogsområdet, vilket visar att en biogen emission från barrträden förkommer. Endast två vattenprover från bakgrundsstationerna hade koncentrationer som översteg detektionsgränserna. Båda dessa var provtagna i skogsområdet under den senare delen av maj när den biogena aktiviteten troligen är hög. Inget av sedimentproverna i det nationella programmet hade koncentrationer överstigande detektionsgränserna. De analyserade reningsverksslammen visade mycket varierande halter. Förhållandet mellan d- och l-limonen varierade också dem emellan vilket tyder på skillnader i antropogen påverkan. Tre prover av utgående vatten från reningsverk analyserades utan några koncentrationer över detektionsgränsen återfanns.
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