| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Li, Xinyu, et al.
(författare)
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Biodegradable MOFilters for Effective Air Filtration and Sterilization by Coupling MOF Functionalization and Mechanical Polarization of Fibrous Poly(lactic acid)
- 2023
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Ingår i: ACS Applied Materials and Interfaces. - : American Chemical Society (ACS). - 1944-8244 .- 1944-8252. ; 15:22, s. 26812-26823
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Tidskriftsartikel (refereegranskat)abstract
- High-performance air filtration materials are importantfor addressingthe airborne pollutants. Herein, we propose an unprecedented accessto biodegradable poly-(lactic acid) (PLA)-based MOFilters with excellentfiltering performance and antibacterial activity. The fabricationinvolved a stepwise in situ growth of zeolitic imidazolate framework-8(ZIF-8) crystals at the surface of microfibrous PLA membranes, followedby mechanical polarization under high pressure and low temperature(5 MPa, 40 degrees C) to trigger the ordered alignment of dipoles inPLA chains and ZIF-8. The unique structural features allowed thesePLA-based MOFilters to achieve an exceptional combination of excellenttensile properties, high dielectric constant (up to 2.4 F/m), andenhanced surface potential as high as 4 kV. Arising from the remarkablesurface activity and electrostatic adsorption effect, a significantincrease (from over 12% to nearly 20%) in PM0.3 filtrationefficiency was observed for the PLA-based MOFilters compared to thatof pure PLA counterparts, with weak relation to the airflow velocities(10-85 L/min). Moreover, the air resistance was controlledat a considerably low level for all the MOFilters, that is, below183 Pa even at 85 L/min. It is worth noting that distinct antibacterialproperties were achieved for the MOFilters, as illustrated by theinhibitive rates of 87 and 100% against Escherichiacoli and Staphylococcus aureus, respectively. The proposed concept of PLA-based MOFilters offersunprecedented multifunction integration, which may fuel the developmentof biodegradable versatile filters with high capturing and antibacterialperformances yet desirable manufacturing feasibility.
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| 4. |
- Liang, Yue, et al.
(författare)
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Late Ordovician lingulid brachiopods from the Pingliang Formation (Shaanxi Province, North China) : Morphological and ecological implications
- 2024
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Ingår i: Journal of Asian Earth Sciences. - : Elsevier. - 1367-9120 .- 1878-5786. ; 263
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Tidskriftsartikel (refereegranskat)abstract
- Brachiopods first appeared in the early Cambrian and persist till present. They are one of the main lineages of marine invertebrates that diversified throughout the Paleozoic and reached their maximum diversity of high-rank taxonomy during the Ordovician. During this time interval, brachiopods were mainly dominated by the articulated Orthida and Strophomenida, which represent major components of the Paleozoic Evolutionary Fauna; however, the inarticulated lingulid brachiopods (Order Lingulida) were comparatively less abundant and received less attention during this time period. Here, we report a new record of the lingulid brachiopod Anomaloglossa porca from the Upper Ordovician (Sandbian) Pingliang Formation of the Xilinggou Section, Shaanxi Province, North China. Collected specimens are preserved as calcium phosphatic shells with highly mineralization which preserve detailed morphology and shell ornamentation of both ventral and dorsal valves. The new occurrence of A. porca extends its paleogeographic distribution from Gondwana and Tarim to North China Platform. Moreover, comprehensive geometric morphometric analysis of A. porca is performed and the results indicate that both the shell shape and pseudointerarea are very close to the recent infaunal lingulids. If interpreted correctly, the fossils represent the first example of infaunal lifestyles achieved by Ordovician lingulids from North China, exhibiting the ecological complexities of the Late Ordovician benthos composed of epibenthos and infaunas as well.
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| 5. |
- Xiao, Tian, et al.
(författare)
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Effect of natural convection on charging of phase change materials in graded metal foam : Pore-scale simulation
- 2023
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Ingår i: International Communications in Heat and Mass Transfer. - 0735-1933. ; 149
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Tidskriftsartikel (refereegranskat)abstract
- Open-cell metal foams (MFs) possess exceptional thermal conductivity and are often utilized to enhance the phase transition efficiency of phase change materials (PCMs) that have insufficient thermal conductivity. The movement of energy within MFs during phase transition is influenced by natural convection and heat conduction. In this study, pore-scale numerical simulation (PNS) was employed to study the effect of natural convection on the phase transition of graded MFs (positive gradient foam-PGF, homogenous foam-HF, and negative gradient foam-NGF). Simplified tetrakaidecahedron cells were used for PNS to capture the primary geometric features of MFs. Results have shown that natural convection significantly influences the phase transition process of PCMs. Compared to pure thermal conductivity, the full melting times (FMTs) of PCMs in PGF, HF, and NGF were reduced by 85.2% - 88.3% when natural convection was considered. Furthermore, the integrated average temperature response rates of PCMs in PGF, HF, and NGF, considering natural convection, were improved by 378.4%, 301.9%, and 342.5%, respectively. The presence of natural convection resulted in a gradient in the phase interface distribution and temperature field. The PNS method proved useful in illustrating the influence of metallic ligaments on phase interface and temperature distributions.
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