| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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| 3. |
- Chen, Yujie, et al.
(författare)
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Unraveling dual phase transformations in a CrCoNi medium-entropy alloy
- 2021
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Ingår i: Acta Materialia. - : Elsevier BV. - 1359-6454 .- 1873-2453. ; 215
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Tidskriftsartikel (refereegranskat)abstract
- The emergence of multi-principal element alloys (MPEAs) holds great promise for the development of high performance metallic materials. However, it remains unclear whether MPEAs can provide previously unknown deformation mechanisms to drastically enhance their mechanical performance. Here we report a new deformation mechanism of mechanically-induced dual phase transformations from the face-centered cubic (FCC) to hexagonal close- packed (HCP) phase and then back to the FCC phase with nanotwins in a CrCoNi medium-entropy alloy (MEA). During the two sequential steps of phase transformation, continued shear occurs in the same < 110 >(FCC) parallel to < 11 (2) over bar0 >(HCP) direction along different {111}(FCC) parallel to (0001)(HCP) planes, producing a total shear transformation strain up to 70%. The dual phase transformations stem from a unique capability of facile slip in between the close-packed {111}(FCC) parallel to (0001)(HCP) atomic layers in both FCC and HCP phases, leading to flexible stacking sequences of those close-packed layers with low stacking fault energies. Our work demonstrates that MPEAs can offer unconventional deformation mechanisms such as dual phase transformations in the CrCoNi MEA, thereby opening opportunities for enhancing the mechanical properties of advanced alloys.
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| 4. |
- Feng, Xingxing, et al.
(författare)
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Human recognition with the optoelectronic reservoir-computing-based micro-Doppler radar signal processing
- 2022
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Ingår i: Applied Optics. - : Optica Publishing Group (formerly OSA). - 1559-128X .- 2155-3165. ; 61:19, s. 5782-5789
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Tidskriftsartikel (refereegranskat)abstract
- Current perception and monitoring systems, such as human recognition, are affected by several environmental factors, such as limited light intensity, weather changes, occlusion of targets, and public privacy. Human recognition using radar signals is a promising direction to overcome these defects; however, the low signal-to-noise ratio of radar signals still makes this task challenging. Therefore, it is necessary to use suitable tools that can efficiently deal with radar signals to identify targets. Reservoir computing (RC) is an efficient machine learning scheme that is easy to train and demonstrates excellent performance in processing complex time-series signals. The RC hardware implementation structure based on nonlinear nodes and delay feedback loops endows it with the potential for real-time fast signal processing. In this paper, we numerically study the performance of the optoelectronic RC composed of optical and electrical components in the task of human recognition with noisy micro-Doppler radar signals. A single-loop optoelectronic RC is employed to verify the application of RC in this field, and a parallel dual-loop optoelectronic RC scheme with a dual-polarization Mach–Zehnder modulator (DPol-MZM) is also used for performance comparison. The result is verified to be comparable with other machine learning tools, which demonstrates the ability of the optoelectronic RC in capturing gait information and dealing with noisy radar signals; it also indicates that optoelectronic RC is a powerful tool in the field of human target recognition based on micro-Doppler radar signals.
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| 5. |
- He, Xiaoyu, et al.
(författare)
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Dual-optimization strategy engineered Ti-based metal-organic framework with Fe active sites for highly-selective CO2 photoreduction to formic acid
- 2023
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Ingår i: Applied Catalysis B. - : Elsevier BV. - 0926-3373 .- 1873-3883. ; 327
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Tidskriftsartikel (refereegranskat)abstract
- Increasing CO2 conversion efficiency over metal-organic framework (MOF) based photocatalysts is of great significance to promote the carbon capture and utilization. In this work, a dual-benefit design strategy is deployed in the synthesis of a new two-dimensional Fe/Ti-BPDC MOF photocatalyst with atomically dispersed Fe sites. This catalyst demonstrated an excellent catalytic performance in the visible-light-driven CO2 conversion to HCOOH, achieving a high yield of 703.9 μmol g-1 h-1 at a selectivity greater than 99.7%. This is attributed to the ‘dual-optimization’ achieved by this catalyst to sustain the supply of photogenerated electrons and to effectively activate CO2. Specifically, the Fe/Ti-BPDC catalyst provides a high proportion of effective photogenerated electrons for the CO2 photocatalysis process via a unique electron transfer mechanism. Meanwhile, the strong O/Fe affinity between CO2 and atomically dispersed Fe active sites not only enables a fast CO2 activation, but also dictates the intermediate reaction pathways towards high HCOOH selectivity.
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| 6. |
- Lou, Xiaolong, et al.
(författare)
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An Integrated Application of Motion Sensing and Eye Movement Tracking Techniques in Perceiving User Behaviors in a Large Display Interaction
- 2023
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Ingår i: Machines. - : MDPI AG. - 2075-1702. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- In public use of a large display, it is a usual phenomenon that multiple users individually participate in respective tasks on a common device. Previous studies have categorized such activity as independent interaction that involves little group engagement. However, by investigating how users approach, participate in, and interact with large displays, we found that parallel use is affected by group factors such as group size and between-user relationship. To gain a thorough understanding of individual and group behaviors, as well as parallel interaction task performance, one 70-inch display-based information searching task and experiment was conducted, in which a mobile eye movement tracking headset and a motion sensing RGB-depth sensor were simultaneously applied. The results showed that (1) a larger group size had a negative influence on the group users’ concentration on the task, perceived usability, and user experience; (2) a close relationship between users contributed to occasional collaborations, which was found to improve the users’ task completion time efficiency and their satisfaction on the large display user experience. This study proves that an integrated application of eye movement tracking and motion sensing is capable of understanding individual and group users’ behaviors simultaneously, and thus is a valid and reliable scheme in monitoring public activities that can be widely used in public large display systems.
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| 7. |
- Lu, Song, et al.
(författare)
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Theory of transformation-mediated twinning
- 2023
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Ingår i: PNAS NEXUS. - : Oxford University Press (OUP). - 2752-6542. ; 2:1
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Tidskriftsartikel (refereegranskat)abstract
- High-density and nanosized deformation twins in face-centered cubic (fcc) materials can effectively improve the combination of strength and ductility. However, the microscopic dislocation mechanisms enabling a high twinnability remain elusive. Twinning usually occurs via continuous nucleation and gliding of twinning partial dislocations on consecutive close-packed atomic planes. Here we unveil a completely different twinning mechanism being active in metastable fcc materials. The transformation-mediated twinning (TMT) is featured by a preceding displacive transformation from the fcc phase to the hexagonal close-packed (hcp) one, followed by a second-step transformation from the hcp phase to the fcc twin. The nucleation of the intermediate hcp phase is driven by the thermodynamic instability and the negative stacking fault energy of the metastable fcc phase. The intermediate hcp structure is characterized by the easy slips of Shockley partial dislocations on the basal planes, which leads to both fcc and fcc twin platelets during deformation, creating more twin boundaries and further enhancing the prosperity of twins. The disclosed fundamental understanding of the complex dislocation mechanism of deformation twinning in metastable alloys paves the road to design novel materials with outstanding mechanical properties.
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| 8. |
- Ye, Kangpeng, et al.
(författare)
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Human Identification by Mean of Optoelectronic Reservoir Computing
- 2022
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Ingår i: 13TH INTERNATIONAL PHOTONICS AND OPTOELECTRONICS MEETINGS (POEM 2021). - : SPIE-Intl Soc Optical Eng.
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Konferensbidrag (refereegranskat)abstract
- As an improvement of the traditional recurrent neural networks (RNN), the reservoir computing (RC) only needs to train one output connection weight matrix linearly, which greatly reduces the number of machine learning network calculations. The optoelectronic RC can be realized with a delay feedback loop composed of optical and electrical devices. It has the advantages of lower power consumption and faster speed than the all-electric RC scheme. At the same time, it is easier to be controlled than the all-optical RC scheme. In this paper, we propose to employ the optoelectronic RC to process radar signals to distinguish different persons in the indoor environment. The radar signal required for the simulation is referred from the IDRad data set, which contains the echo signals of the frequency modulated continuous wave (FMCW) radar, and five persons of different ages are free to move around in the room, which is close to the real scene. First, the echo signal is processed and the micro-Doppler features are extracted, and each frame corresponds to a row vector. Then, this vector is used as the input signal of the optoelectronic RC. We numerically studied the impact of parameters such as the size of the RC and the regularization coefficient in the system. Finally, the classification accuracy of five targets reaches 87%.
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| 9. |
- Zou, Yatao, et al.
(författare)
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Boosting Perovskite Light-Emitting Diode Performance via Tailoring Interfacial Contact
- 2018
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Ingår i: ACS Applied Materials and Interfaces. - : AMER CHEMICAL SOC. - 1944-8244 .- 1944-8252. ; 10:28, s. 24320-24326
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Tidskriftsartikel (refereegranskat)abstract
- Solution-processed perovskite light-emitting diodes (LEDs) have attracted wide attention in the past several years. However, the overall efficiency and stability of perovskite-based LEDs remain inferior to those of organic or quantum dot LEDs. Nonradiative charge recombination and the unbalanced charge injection are two critical factors that limit the device efficiency and operational stability of perovskite LEDs. Here, we develop a strategy to modify the interface between the hole transport layer and the perovskite emissive layer with an amphiphilic conjugated polymer of poly[(9,9-bis(3-(N,N-dimethylamino)propy1)-2,7-fluorene)-alt-2,7-(9,9-dioctylfluorene)] (PFN). We show evidences that PFN improves the quality of the perovskite film, which effectively suppresses nonradiative recombination. By further improving the charge injection balance rate, a green perovskite LED with a champion current efficiency of 45.2 cd/A, corresponding to an external quantum efficiency of 14.4%, is achieved. In addition, the device based on the PFN layer exhibits improved operational lifetime. Our work paves a facile way for the development of efficient and stable perovskite LEDs.
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