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- Li, Peng, et al.
(författare)
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Transcriptional reactivation of OTX2, RX1 and SIX3 during reprogramming contributes to the generation of RPE cells from human iPSCs
- 2016
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Ingår i: International Journal of Biological Sciences. - : Ivyspring International Publisher. - 1449-2288. ; 12:5, s. 505-517
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Tidskriftsartikel (refereegranskat)abstract
- Directed differentiation of human induced pluripotent stem cells (iPSCs) into retinal pigmented epithelium (RPE) holds great promise in cell replacement therapy for patients suffering from degenerative eye diseases, including age-related macular degeneration (AMD). In this study, we generated iPSCs from human dermal fibroblasts (HDFs) by electroporation with episomal plasmid vectors encoding OCT4, SOX2, KLF4, L-MYC together with p53 suppression. Intriguingly, cell reprogramming resulted in a metastable transcriptional activation and selective demethylation of neural and retinal specification-associated genes, such as OTX2, RX1 and SIX3. In contrast, RPE progenitor genes were transcriptionally silent in HDFs and descendant iPSCs. Overexpression of OCT4 and SOX2 directly stimulated the expression of OTX2, RX1 and SIX3 in HDFs and iPSCs. Luciferase and chromatin immunoprecipitation (ChIP) assays further identified an OCT4- and two SOX2-binding sites located in the proximal promoter of OTX2. Histone acetylation and methylation on the local promoter also participated in the reactivation of OTX2. The transcriptional conversion of RX1 and SIX3 genes partially attributed to DNA demethylation. Subsequently, iPSCs were induced into the RPE cells displaying the characteristics of polygonal shapes and pigments, and expressing typical RPE cell markers. Taken together, our results establish readily efficient and safe protocols to produce iPSCs and iPSC-derived RPE cells, and underline that the reactivation of anterior neural transcription factor OTX2, eye field transcription factor RX1 and SIX3 in iPSCs is a feature of pluripotency acquisition and predetermines the potential of RPE differentiation.
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| 2. |
- Chattoraj, Sayantan, et al.
(författare)
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Origin of Deteriorated Crystal Plasticity and Compaction Properties of a 1:1 Cocrystal between Piroxicam and Saccharin
- 2014
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Ingår i: Crystal Growth & Design. - : American Chemical Society (ACS). - 1528-7483 .- 1528-7505. ; 14:8, s. 3864-3874
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Tidskriftsartikel (refereegranskat)abstract
- The 1:1 cocrystal between piroxicam and saccharin exhibits significantly deteriorated powder compaction properties compared to both coformers. The molecular origin of this effect is revealed by a systematic investigation of crystal mechanical properties, probed with nanoindentation, and crystal structure analysis. The order of bulk powder tabletability of the three materials is identical to that of single crystal plasticity (saccharin > piroxicam > cocrystal). The lowest plasticity of the cocrystal is confirmed by its highest crystal hardness and the highest yield strength. The low plasticity of the cocrystal is attributed to structural packing features that discourage plastic deformation. This work demonstrates that cocrystallization, even though it may be useful to improve pharmaceutically relevant properties, must be carefully evaluated to avoid unexpected problems in formulation and drug product manufacturing due to compromised mechanical properties.
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| 3. |
- Kristan, Matej, et al.
(författare)
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The first visual object tracking segmentation VOTS2023 challenge results
- 2023
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Ingår i: 2023 IEEE/CVF International conference on computer vision workshops (ICCVW). - : Institute of Electrical and Electronics Engineers Inc.. - 9798350307443 - 9798350307450 ; , s. 1788-1810
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking Segmentation VOTS2023 challenge is the eleventh annual tracker benchmarking activity of the VOT initiative. This challenge is the first to merge short-term and long-term as well as single-target and multiple-target tracking with segmentation masks as the only target location specification. A new dataset was created; the ground truth has been withheld to prevent overfitting. New performance measures and evaluation protocols have been created along with a new toolkit and an evaluation server. Results of the presented 47 trackers indicate that modern tracking frameworks are well-suited to deal with convergence of short-term and long-term tracking and that multiple and single target tracking can be considered a single problem. A leaderboard, with participating trackers details, the source code, the datasets, and the evaluation kit are publicly available at the challenge website1
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| 6. |
- Yang, Tao, et al.
(författare)
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Regional frequency analysis and spatio-temporal pattern characterization of rainfall extremes in the Pearl River Basin, China
- 2010
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Ingår i: Journal of Hydrology. - : Elsevier BV. - 0022-1694 .- 1879-2707. ; 380:3-4, s. 386-405
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents a method for regional frequency analysis and spatio-temporal pattern characterization of rainfall-extreme regimes (i.e. extremes, durations and timings) in the Pearl River Basin (PRB) using the well-known L-moments approach together with advanced statistical tests including stationarity test and serial correlation check, which are crucial to the valid use of L-moments for frequency analysis. Results indicate that: (1) the entire Pearl River Basin (40 sites) can be categorized into six regions by cluster analysis together with consideration of the topography and spatial patterns of mean precipitation in the basin. The results of goodness-of-fit measures indicate that the GNO, GLO, GEV, and PE3 distributions fit well for most of the basin for different HOM regions, but their performances are slightly different in term of curve fitting; (2) the estimated quantiles and their biases approximated by Monte Carlo simulation demonstrate that the results are reliable enough for the return periods of less than 100 years; (3) excessive precipitation magnitude records are observed at Guilin region of Guangxi Province and Fogang region of Guangdong Province, which have sufficient climate conditions (e.g. precipitation and humidity) responsible for the frequently occurred flood disasters in the regions. In addition, the spatial variations of precipitation in different return periods (Return period = 1, 10, 50 years to 100 years) increase from the upstream to downstream at the regional scale; (4) the seasonal patterns of precipitation extremes for different topographical regions are different. The major precipitation events of AM1R, AM3R, AM5R and AM7R in regions of low-elevation in lower (south-eastern) part of the basin occur mainly in May, June, July and August, while the main precipitation periods for the mountainous region upstream are June, July and August. Further analysis of the NCAR/NCEP reanalysis data indicates that the eastern Asian summer monsoon and typhoons (or hurricanes) are major metrological driving forces on the precipitation regimes. Additionally, topographical features (i.e. elevation, distance to the sea, and mountain’s influences) also exert different impacts on the spatial patterns of such regimes. To the best of our knowledge, this study is the first attempt to conduct a systematic regional frequency analysis on various annual precipitation extremes (based on consecutive 1-, 3-, 5-, 7-day averages) and to establish the possible links to climate pattern and topographical features in the Pearl River Basin and even in China. These findings are expected to contribute to exploring the complex spatio-temporal patterns of extreme rainfall in this basin in order to reveal the underlying linkages between precipitation and floods from a broad geographical perspective. (C) 2009 Published by Elsevier B.V.
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