| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Huang, Wenbin, et al.
(författare)
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A Novel LiDAR-Camera Fused Player Tracking System in Soccer Scenarios
- 2024
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Ingår i: IEEE Sensors Journal. - : Institute of Electrical and Electronics Engineers (IEEE). - 1530-437X .- 1558-1748. ; 24:9, s. 15630-15642
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Tidskriftsartikel (refereegranskat)abstract
- Localization and tracking in multiplayer sports present significant challenges. Existing global positioning system (GPS) and ultra wideband (UWB)-based methods have higher accuracy but require players to wear inconvenient electronic devices. On the other hand, camera-based solutions are device-free, but have unsatisfied accuracy and necessitate significant manual intervention. Thus, we introduced a light detection and ranging (LiDAR)-camera fused system tailored for soccer scenarios, which can combine highest position accuracy and convenient application. With this continuous data collection system, a new benchmark dataset is constructed, and a simple and effective tracking-by-detection method is designed. The method comprises two main modules. The cascade PointNet module effectively identifies players from the point cloud, while the subsequent Tracklet-Zoom module efficiently performs tracklet association. Our method shows significant improvements in multiple evaluation metrics compared with other methods. Specifically, we achieved a score of 95.21% on the GT-consistency metric, representing a 13.29% increase. This indicates that our method has made substantial progress toward achieving automated tracking.
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| 3. |
- Wang, Hongya, et al.
(författare)
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Comparing mineral magnetic properties of sediments in two reservoirs in "strongly" and "mildly" eroded regions on the Guizhou Plateau, southwest China: A tool for inferring differences in sediment sources and soil erosion
- 2011
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Ingår i: Geomorphology. - : Elsevier BV. - 0169-555X. ; 130:3-4, s. 255-271
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Tidskriftsartikel (refereegranskat)abstract
- Shibanqiao Reservoir (0.24 km(2)) and Xiaohe Reservoir (0.28 km(2)) have catchments of 6.00 km(2) and 22.69 km(2), respectively. They essentially represent "strongly eroded" (Shibanqiao) and "mildly eroded" (Xiaohe) regions in Guizhou Plateau, southwestern China. Sediment cores were retrieved from the two reservoirs and soils were also sampled from their catchments. Mineral magnetic measurements were performed on the sediments and soils, and the particle-size analysis of selected sediment cores and soil profiles were also made. SIRM versus chi if was used to compare sediment and soil samples. In addition, correlations between percentages of different particle-size fractions and magnetic parameters/ratios of sediment samples were also checked. These results revealed differences in sediment sources and soil erosion in these two representative catchments. The sediments in Shibanqiao Reservoir are derived from both the topsoil and subsoil. When erosion is relatively weak, it is limited to the topsoil and preferentially transports the relatively finer magnetic grains into the reservoir, which may imply rain splash and/or sheet erosion. In this relatively steep catchment, stronger erosional forces are capable of not only moving relatively coarser ferrimagnetic grains from the topsoil, but also incising the subsoil and moving anti-ferromagnetic minerals into the reservoir, which seems to hint at not only sheet-wash but also rill and gully erosion. As there are no stable streams nor channel networks developed in this mountainous catchment, contributions from river-bank erosion to sediment yield are almost negligible. The sediments in Xiaohe Reservoir, which has a lower relief, are predominately derived from topsoil erosion. Here, erosion is concentrated to the topsoil and preferentially transports the relatively fine magnetic grains into the reservoir when erosional forces are weak. However, even when erosional forces increase in this relatively flat catchment, erosion is still restricted to the topsoil. Thus, relatively coarser ferrimagnetic grains from the topsoil are transported to the reservoir, but the subsoil remains undisturbed and there are no significant changes in the proportion of anti-ferromagnetic minerals in the sediments. Consequently, only sheet-washing occurs even when erosion is intensifying in this hilly plain catchment. In general, the more effective erosion in the Shibanqiao Catchment is probably due to its steeper topography than Xiaohe Catchment, although higher and more seasonal rainfalls and lower percentages of woodlands may also contribute to the differences in susceptibility to erosional forces. (C) 2011 Elsevier B.V. All rights reserved.
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