| 1. |
- Abreu, Ilka, et al.
(författare)
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A metabolite roadmap of the wood-forming tissue in Populus tremula
- 2020
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Ingår i: New Phytologist. - : John Wiley & Sons. - 0028-646X .- 1469-8137. ; 228:5, s. 1559-1572
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Tidskriftsartikel (refereegranskat)abstract
- Wood, or secondary xylem, is the product of xylogenesis, a developmental process that begins with the proliferation of cambial derivatives and ends with mature xylem fibers and vessels with lignified secondary cell walls. Fully mature xylem has undergone a series of cellular processes, including cell division, cell expansion, secondary wall formation, lignification and programmed cell death. A complex network of interactions between transcriptional regulators and signal transduction pathways controls wood formation. However, the role of metabolites during this developmental process has not been comprehensively characterized. To evaluate the role of metabolites during wood formation, we performed a high spatial resolution metabolomics study of the wood-forming zone of Populus tremula, including laser dissected aspen ray and fiber cells. We show that metabolites show specific patterns within the wood-forming zone, following the differentiation process from cell division to cell death. The data from profiled laser dissected aspen ray and fiber cells suggests that these two cell types host distinctly different metabolic processes. Furthermore, by integrating previously published transcriptomic and proteomic profiles generated from the same trees, we provide an integrative picture of molecular processes, for example, deamination of phenylalanine during lignification is of critical importance for nitrogen metabolism during wood formation.
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| 2. |
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| 3. |
- Blåvarg, Ebba Christina, 1967-
(författare)
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Psykologi på schemat : Formeringen av ett skolämne, 1960–2015
- 2023
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The school subject psychology in Sweden has undergone several extensive transformations during the period 1960–2015. Since the launch of Läroplan för gymnasiet 1965, psychology is a part of the national curriculum for upper secondary level as a standalone school subject in Sweden. However, it has been formulated differently within different discursive practices. This thesis focuses on the formation of the school subject psychology by analyzing texts on the subject from mainly three different practices where the subject is constructed. The first is a bureaucratic space that includes public policy texts developed through cooperation and regulation by way of bureaucratic processes. The second is a practice-oriented space and it includes teaching materials such as textbooks produced by publishers and individual authors. The third practice is a professional space and includes the discourse on the school subject psychology among teachers in the subject teacher’s association, teacher magazines and debate articles. Through a discursive perspective, the source material from these practices have been analyzed both by focusing on each space separately, and in parallel. Overall, the discourse on the school subject psychology has changed dynamically during the focused period, in the different spaces, which is depicted in detail with examples and excerpts. In addition, four overarching discourse orders are presented. First, the overarching empirical and scientific stance that permeated the subject at the beginning of the national curriculum in the 1960s. Later, in the 1990s and 2000s, an inward shift in subject focus, from being useful for understanding the world with a critical view, to critically examining and evaluating various psychological theories. However, perhaps the most unexpected finding are two major orders of discourse that can be seen in between these in time. Firstly, an overall vivid activity in all the practices and the major change that the subject underwent in the 1970s, in conjunction with the subject revision in 1979. And secondly, the extensive and almost absolute silence that followed in relation to the subject psychology. This thesis contributes to the research field by mapping the formation of psychology as a school subject in Sweden through different discursive practices, as well as highlighting various orders of discourse in relation to psychology as a school subject.
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| 4. |
- Brackmann, Christian, 1973, et al.
(författare)
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In situ Imaging of Collagen Synthesis by Osteoprogenitor Cells in Microporous Bacterial Cellulose Scaffolds.
- 2012
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Ingår i: Tissue Engineering - Part C: Methods. - 1937-3384 .- 1937-3392. ; 18:3, s. 227-234
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Tidskriftsartikel (refereegranskat)abstract
- Microscopy techniques based on laser-induced nonlinear optical processes allow for chemically specific imaging of unmodified samples at high spatial resolution in three dimensions and provide powerful tools for characterization of tissue-engineering constructs. This is highlighted by the simultaneous imaging of scaffold material, cells, and produced extracellular matrix collagen in samples consisting of osteoprogenitor MC3T3-E1 cells seeded on microporous bacterial cellulose (BC), a potential scaffold material for synthesis of osseous tissue. BC and collagen have been visualized by second harmonic generation (SHG) microscopy, and verification of collagen identification on cellulose scaffolds has been carried out on sectioned samples by comparison with the conventional histological staining technique. Both methods showed similar collagen distributions and a clear increase in the amount of collagen when comparing measurements from two time points during growth. For investigations of intact cellulose scaffolds seeded with cells, SHG was combined with simultaneous coherent anti-Stokes Raman scattering (CARS) microscopy for visualization of cell arrangement in three dimensions and to be correlated with the SHG data. Results showed that the osteoprogenitor cells were able to produce collagen already during the first days of growth. Further on, developed collagen fiber networks could be imaged inside compact regions of cells located in the cellulose micropores. Collagen production, the initial step of tissue mineralization, demonstrates the potential of BC as a scaffold material for bone tissue engineering. Furthermore, the noninvasive in situ monitoring of collagen inside compact tissue clearly manifests the benefits of nonlinear microscopy techniques, such as SHG and CARS, for use in tissue engineering.
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| 5. |
- Haenni, Arvo, et al.
(författare)
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Effect of fat emulsion (Fabuless) on orocecal transit time in healthy men
- 2009
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Ingår i: Scandinavian Journal of Gastroenterology. - : Informa UK Limited. - 0036-5521 .- 1502-7708. ; 44:10, s. 1186-1190
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Tidskriftsartikel (refereegranskat)abstract
- Background. Given the growing prevalence of overweight and related health consequences, there is increased interest in the search for novel dietary strategies for weight control. A food ingredient, an emulsion based on palm and oat oil (Fabuless, previously known as Olibra), has been associated with short-term reductions of food intake, induction of satiety, alternation in the satiety hormones, as well as long-term effects on weight control. The mechanism by which it can exert these effects is so far unclear, though it has been suggested that the "ileal break'' may play a role in increasing gastrointestinal transit time. The aim of this study was to investigate the effects of this stable fat emulsion on orocecal transit time in healthy men. Material and methods. In a controlled, double-blind, cross-over-designed study, 15 healthy men (aged 20-59 years, body mass index (BMI) 22-28), randomly allocated to two treatments, consumed the stable fat emulsion or a milk fat in yoghurt during two days of investigation, with an interval of 1 week. Orocecal transit time was determined by following blood sulfapyridine levels, which is a metabolite of salazopyrine in the colon. Results. A statistically significant delay in the appearance of sulfapyridine in serum was obtained after active treatment versus control treatment, corresponding to a 45-min longer orocecal transit time due to fat emulsion consumption. Conclusions. This study provides the first evidence to suggest that this stable fat emulsion may affect the ileal brake mechanism by slowing down the gastrointestinal transit time, which might explain the weight control and appetite suppression previously observed in association with this emulsion.
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| 6. |
- Hansson, Markus, et al.
(författare)
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A phase 1 dose-escalation study of antibody BI-505 in relapsed/refractory multiple myeloma.
- 2015
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Ingår i: Clinical Cancer Research. - 1078-0432. ; 21:12, s. 2730-2736
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: This multicenter, first-in-human study evaluated safety, tolerability, pharmacokinetics, and pharmacodynamics of BI-505, a human anti-ICAM-1 monoclonal antibody, in advanced relapsed/refractory multiple myeloma patients. Experimental design: BI-505 was given intravenously, every two weeks, at escalating doses from 0.0004 to 20 mg/kg, with extension of therapy until disease progression for responding or stable patients receiving 0.09 mg/kg or higher doses. Results: A total of 35 patients were enrolled. The most common adverse events were fatigue, pyrexia, headache, and nausea. Adverse events were generally mild to moderate and those attributed to study medication were mostly limited to the first dose, and manageable with premedication and slower infusion. No maximum tolerated dose was identified. BI-505's half-life increased with dose while clearance decreased, suggesting target-mediated clearance. The ICAM-1 epitopes on patient bone marrow myeloma were completely saturated at 10 mg/kg doses. Using the International Myeloma Working Group criteria, seven patients on extended therapy had stable disease for more than two months. Conclusion: BI-505 can be safely administered at doses that saturate myeloma cell ICAM-1 receptors in patients. This study was registered at www.clinicaltrials.gov (NCT01025206).
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| 7. |
- Hasselblad, Annika, 1991-, et al.
(författare)
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Making techno-economic rationality work : tensions in technology-enabled social service evaluations
- 2024
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Ingår i: Journal of technology in human services. - : Routledge. - 1522-8835 .- 1522-8991. ; 42:1, s. 1-24
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Tidskriftsartikel (refereegranskat)abstract
- Contemporary welfare organizations engage in various evaluation practices to assess the quality of their services. In this paper we report a qualitative exploration of how technology-enabled evaluations are understood by organizational members who participate in quality assurance activities in Swedish social services. The study contributes to critical information systems literature, focusing on the tensions professionals experience in relation to the digital systems they use for evaluations. For example, "quantities" take precedence over the qualities of such work, as information systems constrain ambitions to realize knowledge-based social services. The results reveal three tensions in professionals' evaluation-related activities arising from conflicting uses or desires. One is between desires for flexible systems that enable reflection and standardized digital support systems. Another is between uses or desires for indicators that are meaningful at the operational level and for general, comparable measures at the management level. The third is between desires to use evaluation procedures for learning and control. The study contributes to both theory and practice related to technology-enabled evaluation of welfare services, and critical perspectives on information systems.
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| 8. |
- Hasselblad, Annika, 1991-, et al.
(författare)
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When Worlds Collide : Comparing the Logic of the Industrial and Welfare Societies
- 2020
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Ingår i: EGOV-CeDEM-ePart 2020. - : Digital government society. ; , s. 317-326
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Konferensbidrag (refereegranskat)abstract
- As governments around the world are increasingly using digital technology to generate social value, it is important to consider what types of logic underpin this value creation. Previous research has identified a dominant market logic, associated with new public management (NPM) in the public sector, which has had certain adverse effects. Against this backdrop, the purpose of this paper is to outline and compare the logic of two ideal societies: the industrial society and the welfare society. The study examines core works of literature on these two ‘ideal-type’ societies, each with its own logic. The results reveal important differences between these societies. While the welfare society neglects factors related to the mode of production and time, the industrial society lacks several important values, including human rights, solidarity, community and equality. By comparing these societies, this paper generates an understanding of why it is highly problematic for an industrial-based NPM logic to dominate in sectors such as education and health care. Such dominance is likely to lead to important values being overlooked.
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| 9. |
- Hellberg, Lena, et al.
(författare)
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Ett nationellt sammanhållet system för kunskapsbaserad vård : ett system, många möjligheter
- 2020
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Utredningens förslag har primärt två syften. Förslagen syftar dels till att öka förutsättningarna för staten att göra de insatser som mest effektivt bidrar till att målen med hälso- och sjukvården nås, dels till att öka förutsättningarna för hälso- och sjukvårdens professioner att arbeta kunskapsbaserat.Hälso- och sjukvården är en komplex verksamhet med många olika aktörer. För att få ihop hälso- och sjukvården till en fungerande helhet behövs strukturer och väl fungerande samarbeten.Utredningens uppdrag handlar i grunden om att utveckla och stärka strukturer, förutsättningar och samarbeten för att nå en god vård. Utredningens samlade förslag, bedömningar och rekommendationer bidrar till bättre förutsättningar för en långsiktig utveckling där staten, regionerna och kommunerna kan arbeta tillsammans mot målen med hälso- och sjukvården och därmed skapa värde för patienten. Detta görs genom att:• underlätta för regeringen att styra mer strategiskt, kunskapsbaserat och långsiktigt hållbart. En förbättrad uppföljning av statliga initiativ och ett mer strukturerat samarbete mellan myndigheterna bidrar till att skapa ett utvecklat underlag för regeringens beslut om nya insatser.• förutsättningar för att arbeta kunskapsbaserat lokalt i regioner och kommuner stärks bl.a. genom att staten fortsätter att utveckla sitt stöd. Mer fokus än i dag läggs på stöd till den kommunala hälso- och sjukvården.• ett nationellt sammanhållet system för kunskapsbaserad vård får långsiktiga förutsättningar att utvecklas genom att en överenskommelse sluts mellan stat, regioner och kommuner. Principer och strukturer för ett långsiktigt samarbete läggs fast där några centrala utgångspunkter är en gemensam målbild samt principer för prioriterings- och arbetsprocesser.
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| 10. |
- Liungman, Annika, et al.
(författare)
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Växtplankton i Södermanlands län 2017
- 2019
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- På uppdrag av Länsstyrelsen i Södermanland har Medins Havs och Vattenkon-sulter under 2017 provtagit och analyserat växtplankton från 37 sjöar i Söder-manlands län. Undersökningen genomfördes främst i syfte att bedöma närings-statusen i sjöarna. Provtagning och analys utfördes enligt standardiserad me-tod (Havs- och vattenmyndigheten 2016, SS-EN 16698:2015 och SS-EN 15204: 2006) och gällande bedömningsgrunder (Havs- och vattenmyndigheten 2013). I denna rapport presenteras resultaten från provtagningen, laboratorieanalysen och statusklassificeringen.Växtplankton i sjöar studeras inom miljöövervakningen av främst två skäl. Dels för att växtplanktonsamhällets mängd och sammansättning avspeglar närings-tillståndet i den aktuella sjön. Dels kan en del växtplankton själva bli ett direkt problem som t.ex. vid toxiska algblomningar eller om problemskapande arter uppträder i dricksvattentäkter. I denna undersökning studerades växtplankton främst av det första skälet.Artsammansättningen hos växtplankton varierar mellan olika typer av sjöar. Viktiga faktorer som styr artsammansättning och biomassa är bl.a. näringstill-gång, ljus, temperatur, humushalt, pH och det övriga ekosystemets samman-sättning, t.ex. artsammansättning och biomassa av fisk, djurplankton och un-dervattensvegetation. När någon av ovanstående faktorer ändras kan det på-verka växtplanktonsamhället och eftersom växtplankton är relativt kortlivade organismer kan förändringar ske snabbt. Eftersom olika växtplanktonarter har olika krav på omvärldsförhållandena kan man genom att studera växtplankton-samhället få information om framförallt sjöars näringssituation och surhet.
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