| 1. |
- Ahvenniemi, Esko, et al.
(författare)
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Recommended reading list of early publications on atomic layer deposition-Outcome of the "Virtual Project on the History of ALD"
- 2017
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Ingår i: Journal of Vacuum Science & Technology. A. Vacuum, Surfaces, and Films. - : American Vacuum Society. - 0734-2101 .- 1520-8559. ; 35:1
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Forskningsöversikt (refereegranskat)abstract
- Atomic layer deposition (ALD), a gas-phase thin film deposition technique based on repeated, self-terminating gas-solid reactions, has become the method of choice in semiconductor manufacturing and many other technological areas for depositing thin conformal inorganic material layers for various applications. ALD has been discovered and developed independently, at least twice, under different names: atomic layer epitaxy (ALE) and molecular layering. ALE, dating back to 1974 in Finland, has been commonly known as the origin of ALD, while work done since the 1960s in the Soviet Union under the name "molecular layering" (and sometimes other names) has remained much less known. The virtual project on the history of ALD (VPHA) is a volunteer-based effort with open participation, set up to make the early days of ALD more transparent. In VPHA, started in July 2013, the target is to list, read and comment on all early ALD academic and patent literature up to 1986. VPHA has resulted in two essays and several presentations at international conferences. This paper, based on a poster presentation at the 16th International Conference on Atomic Layer Deposition in Dublin, Ireland, 2016, presents a recommended reading list of early ALD publications, created collectively by the VPHA participants through voting. The list contains 22 publications from Finland, Japan, Soviet Union, United Kingdom, and United States. Up to now, a balanced overview regarding the early history of ALD has been missing; the current list is an attempt to remedy this deficiency.
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| 2. |
- Bergquist, Jonas, et al.
(författare)
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Mass spectrometry of proteins - Uppsala perspectives on past and present : Paper in honor of Prof. Peter Roepstorff's 65th birthday
- 2007
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Ingår i: International Journal of Mass Spectrometry. - : Elsevier BV. - 1387-3806 .- 1873-2798. ; 268:2-3, s. 73-82
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Tidskriftsartikel (refereegranskat)abstract
- The development of biological mass spectrometry has been rapid in the past three to four decades. In particular, the possibility to detect and identify peptides and proteins from biologically and medically relevant samples has revolutionized life sciences. The development has gone from a stage where the detection of insulin in a mass spectrum was a major event to one in which the recording of mass spectra with more than 104 resolved and calibrating peaks in each spectrum is a routine task.In this paper, the evolution of protein mass spectrometry will be discussed from the Uppsala horizon with special emphasis on the unique coupling between ion induced desorption of biomolecules and ion track physics.
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| 3. |
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| 4. |
- Dahlgren, Claes, 1949, et al.
(författare)
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G protein coupled pattern recognition receptors expressed in neutrophils: Recognition, activation/modulation, signaling and receptor regulated functions.
- 2022
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Ingår i: Immunological reviews. - : Wiley. - 1600-065X .- 0105-2896. ; 314:1, s. 69-92
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Forskningsöversikt (refereegranskat)abstract
- Neutrophils, the most abundant white blood cell in human blood, express receptors that recognize damage/microbial associated pattern molecules of importance for cell recruitment to sites of inflammation. Many of these receptors belong to the family of G protein coupled receptors (GPCRs). These receptor-proteins span the plasma membrane in expressing cells seven times and the down-stream signaling rely in most cases on an activation of heterotrimeric G proteins. The GPCRs expressed in neutrophils recognize a number of structurally diverse ligands (activating agonists, allosteric modulators, and inhibiting antagonists) and share significant sequence homologies. Studies of receptor structure and function have during the last 40years generated important information on GPCR biology in general; this knowledge aids in the overall understanding of general pharmacological principles, governing regulation of neutrophil function and inflammatory processes, including novel leukocyte receptor activities related to ligand recognition, biased/functional selective signaling, allosteric modulation, desensitization, and reactivation mechanisms as well as communication (receptor transactivation/cross-talk) between GPCRs. This review summarizes the recent discoveries and pharmacological hallmarks with focus on some of the neutrophil expressed pattern recognition GPCRs. In addition, unmet challenges, including recognition by the receptors of diverse ligands and how biased signaling mediate different biological effects are described/discussed.
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| 5. |
- Dahlgren, Claes, 1949, et al.
(författare)
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Neutrophil Signaling That Challenges Dogmata of G Protein-Coupled Receptor Regulated Functions
- 2020
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Ingår i: ACS Pharmacology and Translational Science. - : American Chemical Society (ACS). - 2575-9108. ; 3:2, s. 203-220
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Forskningsöversikt (refereegranskat)abstract
- Activation as well as recruitment of neutrophils, the most abundant leukocyte in human blood, to sites of infection/inflammation largely rely on surface-exposed chemoattractant receptors. These receptors belong to the family of 7-transmembrane domain receptors also known as G protein-coupled receptors (GPCRs) due to the fact that part of the downstream signaling relies on an activation of heterotrimeric G proteins. The neutrophil GPCRs share significant sequence homologies but bind many structurally diverse activating (agonistic) and inhibiting (antagonistic) ligands, ranging from fatty acids to purines, peptides, and lipopeptides. Recent structural and functional studies of neutrophil receptors have generated important information on GPCR biology in general; this knowledge aids in the overall understanding of general pharmacological principles, governing regulation of neutrophil function and inflammatory processes, including novel leukocyte receptor activities related to ligand recognition, biased/functional selective signaling, allosteric modulation, desensitization mechanisms and reactivation, and communication (cross-talk) between GPCRs. This review summarizes the recent discoveries and pharmacological hallmarks with focus on neutrophil GPCRs. In addition, unmet challenges are dealt with, including recognition by the receptors of diverse ligands and how biased signaling mediates different biological effects. © 2020 American Chemical Society.
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| 6. |
- Ek, Mats, et al.
(författare)
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Avfall från skogsindustrin- mängder, sammansättning och omhändertagande
- 1996
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- En genomgång av mängder, sammansättning och omhändertagande av vissa typer av avfall från den svenska massa- och pappersindustrin har gjorts. I rapporten finns en tabell som sammanfattar de viktigaste resultaten. Övrigt omhändertagande för kemiskt slam och bioslam är huvudsakligen som jordförbättringsmedel. Det uttagna överskottet av elfilterstoft från sodapannan går till avlopp. Metallanalyser har utförts på ett stort antal slam för att ge underlag för bedömningar av lämpligt omhändertagande. Mängden lakvatten från egna deponier uppskattades 1994 till ca 2 000 000 m3. Av den mängden går 25-30 % till brukens biologiska rening, där den akuta toxiciteten troligen elimineras. Rapporten innehåller beskrivning av förbehandling (t ex avvattning), diskussion av alternativt omhändertagande av de olika avfallen, och en liten internationell utblick.
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| 7. |
- Emsing, Mikael, 1980-, et al.
(författare)
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Swedish Police Officers’ Perceptions of Conflict Management Training in School and Probationary Training
- 2020
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Ingår i: Nordic Journal of Studies in Policing. - : Universitetsforlaget. - 2703-7045. ; 7:2, s. 80-98
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study is to increase knowledge of how police officers define conflict and conflict management as well as how they perceive their training in conflict management, in relation to previous experiences, in-school training and their probationary training. Swedish police officers (n = 20) who had recently finished their probationary training were interviewed focusing on conflict and conflict management. The study shows that the respondents had general descriptions of conflict, which focused almost solely on interpersonal conflict. Further, the development of adaptive conflict behaviors during probationary training was largely dependent on their instructors, whose role and tasks are very complex. In addition, respondents reported an accelerated maturation process of sorts, in which they described themselves as less naïve and more cynical, despite their short time at work. The findings in this study might provide valuable insights into how police officers perceive conflict and conflict management.
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| 8. |
- Forsman, Huamei, et al.
(författare)
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AZ2158 is a more potent formyl peptide receptor 1 inhibitor than the commonly used peptide antagonists in abolishing neutrophil chemotaxis.
- 2023
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Ingår i: Biochemical pharmacology. - : Elsevier BV. - 1873-2968 .- 0006-2952. ; 211
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Tidskriftsartikel (refereegranskat)abstract
- Formyl peptide receptor 1 (FPR1), a G protein-coupled receptor expressed in phagocytes, recognizes short N-formylated peptides originating from proteins synthesized by bacteria and mitochondria. Such FPR1 agonists are important regulators of neutrophil functions and by that, determinants of inflammatory reactions. As FPR1 is implicated in promoting both pro-inflammatory and pro-resolving responses associated with inflammatory diseases, characterization of ligands that potently and selectively modulate FPR1 induced functions might be of high relevance. Accordingly, a number of FPR1 specific antagonists have been identified and shown to inhibit agonist binding or receptor down-stream signaling as well as neutrophil functions such as granule secretion and NADPH oxidase activity. The inhibitory effect on neutrophil chemotaxis induced by FPR1 agonists has generally not been part of basic antagonist characterization. In this study we show that the inhibitory effects on neutrophil chemotaxis of established FPR1 antagonists (i.e., cyclosporin H, BOC1 and BOC2) are limited. Our data demonstrate that the recently described small molecule AZ2158 is a potent and selective FPR1 antagonist in human neutrophils. In contrast to the already established FPR1 antagonists, AZ2158 also potently inhibits chemotaxis. Whereas the cyclosporin H inhibition was agonist selective, AZ2158 inhibited the FPR1 response induced by both a balanced and a biased FPR1 agonist equally well. In accordance with the species specificity described for many FPR1 ligands, AZ2158 was not recognized by the mouse orthologue of FPR1. Our data demonstrate that AZ2158 may serve as an excellent tool compound for further mechanistic studies of human FPR1 mediated activities.
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| 9. |
- Forsman, Huamei, et al.
(författare)
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Function and regulation of GPR84 in human neutrophils
- 2024
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Ingår i: British Journal of Pharmacology. - : Wiley. - 0007-1188 .- 1476-5381. ; 181:10, s. 1536-1549
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Forskningsöversikt (refereegranskat)abstract
- Human neutrophils are components of the innate immune system and are the most abundant white blood cells in the circulation. They are professional phagocytes and express several G protein-coupled receptors (GPCRs), which are essential for proper neutrophil functions. So far, the two formyl peptide receptors, FPR1 and FPR2, have been the most extensively studied group of neutrophil GPCRs, but recently, a new group, the free fatty acid (FFA) receptors, has attracted growing attention. Neutrophils express two FFA receptors, GPR84 and FFA2, which sense medium- and short-chain fatty acids respectively, and display similar activation profiles. The exact pathophysiological role of GPR84 is not yet fully understood, but it is generally regarded as a pro-inflammatory receptor that mediates neutrophil activation. In this review, we summarize current knowledge of how GPR84 affects human neutrophil functions and discuss the regulatory mechanisms that control these responses, focusing on the similarities and differences in comparison to the two FPRs and FFA2.
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| 10. |
- Fredriksson, Johanna, et al.
(författare)
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GRK2 selectively attenuates the neutrophil NADPH-oxidase response triggered by beta-arrestin recruiting GPR84 agonists
- 2022
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Ingår i: Biochimica Et Biophysica Acta-Molecular Cell Research. - : Elsevier BV. - 0167-4889. ; 1869:7
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Tidskriftsartikel (refereegranskat)abstract
- In order to avoid a prolonged pro-inflammatory neutrophil response, signaling downstream of an agonist-activated G protein-coupled receptor (GPCR) has to be rapidly terminated. Among the family of GPCR kinases (GRKs) that regulate receptor phosphorylation and signaling termination, GRK2, which is highly expressed by immune cells, plays an important role. The medium chain fatty acid receptor GPR84 as well as formyl peptide receptor 2 (FPR2), receptors expressed in neutrophils, play a key role in regulating inflammation. In this study, we investigated the effects of GRK2 inhibitors on neutrophil functions induced by GPR84 and FPR2 agonists. GRK2 was shown to be expressed in human neutrophils and analysis of subcellular fractions revealed a cytosolic localization. The GRK2 inhibitors enhanced and prolonged neutrophil production of reactive oxygen species (ROS) induced by GPR84-but not FPR2-agonists, suggesting a receptor selective function of GRK2. This suggestion was supported by beta-arrestin recruitment data. The ROS production induced by a non beta-arrestin recruiting GPR84 agonist was not affected by the GRK2 inhibitor. Termination of this beta-arrestin independent response relied, similar to the response induced by FPR2 agonists, primarily on the actin cytoskeleton. In summary, we show that GPR84 utilizes GRK2 in concert with beta-arrestin and actin cytoskeleton dependent processes to fine-tune the activity of the ROS generating NADPH-oxidase in neutrophils.
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