| 1. |
- Manneberg, Otto, et al.
(författare)
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A three-dimensional ultrasonic cage for characterization of individual cells
- 2008
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Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 93, s. 063901-
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate enrichment, controlled aggregation, and manipulation of microparticles and cells by an ultrasonic cage integrated in a microfluidic chip compatible with high-resolution optical microscopy. The cage is designed as a dual-frequency resonant filleted square box integrated in the fluid channel. Individual particles may be trapped three dimensionally, and the dimensionality of one-dimensional to three-dimensional aggregates can be controlled. We investigate the dependence of the shape and position of a microparticle aggregate on the actuation voltages and aggregate size, and demonstrate optical monitoring of individually trapped live cells with submicrometer resolution.
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| 2. |
- Manneberg, Otto, 1981-, et al.
(författare)
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Spatial confinement of ultrasonic force fields in microfluidic chips
- 2009
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Ingår i: Ultrasonics. - : Elsevier BV. - 0041-624X .- 1874-9968. ; 49, s. 112-119
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate and investigate multiple localized ultrasonic manipulation functions in series in microfluidic chips. The manipulation functions are based on spatially separated and confined ultrasonic primary radiation force fields, obtained by local matching of the resonance condition of the microfluidic channel. The channel segments are remotely actuated by the use of frequency-specific external transducers with refracting wedges placed on top of the chips. The force field in each channel segment is characterized by the use of micrometer-resolution particle image velocimetry ( micro-PIV). The confinement of the ultrasonic fields during single-or dual-segment actuation, as well as the cross-talk between two adjacent. fields, is characterized and quantified. Our results show that the field confinement typically scales with the acoustic wavelength, and that the cross-talk is insignificant between adjacent. fields. The goal is to define design strategies for implementing several spatially separated ultrasonic manipulation functions in series for use in advanced particle or cell handling and processing applications. One such proof-of-concept application is demonstrated, where. flow-through-mode operation of a chip with. flow splitting elements is used for two-dimensional pre-alignment and addressable merging of particle tracks.
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| 3. |
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| 4. |
- Manneberg, Otto, 1981-, et al.
(författare)
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Ultrasonic micro-cages : A new approach for manipulation and monitoring of individual cells and for fluid mixing
- 2008
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Ingår i: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. - : Chemical and Biological Microsystems Society. ; , s. 1495-1497
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Konferensbidrag (refereegranskat)abstract
- Four designs of ultrasonic microcages are presented together with force field simulations and experimental verification. The microcages enable three-dimensional ultrasonic manipulation of individual microparticles combined with on-line monitoring using high-resolution optical microscopy. The microcages can also be employed as acoustic-streaming-based micromixers. We investigate and compare the force field distributions and streaming patterns in the cages, and we demonstrate concentration, aggregation and positioning of individual particles. The cages can be used for, e.g., studies of interactions between single cells and functionalized particles or pairs of cells in contact only with each other.
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| 5. |
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| 6. |
- Manneberg, Otto, et al.
(författare)
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Wedge transducer design for two-dimensional ultrasonic manipulation in a microfluidic chip
- 2008
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Ingår i: Journal of Micromechanics and Microengineering. - : IOP Publishing. - 0960-1317 .- 1361-6439. ; 18, s. 095025-
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Tidskriftsartikel (refereegranskat)abstract
- We analyze and optimize the design of wedge transducers used for the excitation of resonances in the channel of a microfluidic chip in order to efficiently manipulate particles or cells in more than one dimension. The design procedure is based on (1) theoretical modeling of acoustic resonances in the transducer-chip system and calculation of the force fields in the fluid channel, (2) full-system resonance characterization by impedance spectroscopy and (3) image analysis of the particle distribution after ultrasonic manipulation. We optimize the transducer design in terms of actuation frequency, wedge angle and placement on top of the chip, and we characterize and compare the coupling effects in orthogonal directions between single- and dual-frequency ultrasonic actuation. The design results are verified by demonstrating arraying and alignment of particles in two dimensions. Since the device is compatible with high-resolution optical microscopy, the target application is dynamic cell characterization combined with improved microfluidic sample transport.
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| 7. |
- Svennebring, Jessica, et al.
(författare)
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Dynamic flow cytometry in an acousto-optic microfluidic chip
- 2008
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Ingår i: 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. - : Chemical and Biological Microsystems Society. ; , s. 1175-1177
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Konferensbidrag (refereegranskat)abstract
- A microfluidic ultrasonic manipulation system is used for controlled selection, re-tention and optical characterization of individual particles or cells. The system is based on a chip-integrated focused ultrasonic resonator that is actuated on either one or both of two different frequencies. Particles are pre-aligned, and either bypassed through or injected and retained in the focused resonator, depending on the actuation mode. Dynamic flow cytometry is demonstrated by real-time optical monitoring of controlled numbers of retained and positioned cells or beads.
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| 8. |
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| 9. |
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| 10. |
- Svennebring, Jessica, et al.
(författare)
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Selective bioparticle retention and characterization in a chip-integrated confocal ultrasonic cavity
- 2009
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Ingår i: Biotechnology and Bioengineering. - : Wiley. - 0006-3592 .- 1097-0290. ; 103, s. 323-328
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate selective retention and positioning of cells or other bioparticles by ultrasonic manipulation in a microfluidic expansion chamber during microfluidic perfusion. The chamber is designed as a confocal ultrasonic resonator for maximum confinement of the ultrasonic force field at the chamber center, where the cells are trapped. We investigate the resonant modes in the expansion chamber and its connecting inlet channel by theoretical modeling and experimental verification during no-flow conditions. Furthermore, by triple-frequency ultrasonic actuation during continuous microfluidic sample feeding, a set of several manipulation functions performed in series is demonstrated: sample bypass-injection-aggregation and retention-positioning. Finally, we demonstrate transillumination microscopy imaging Of Ultrasonically trapped COS-7 cell aggregates.
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