| 1. |
- Aguetoni Cambui, Camila, et al.
(författare)
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Patterns of Plant Biomass Partitioning Depend on Nitrogen Source
- 2011
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Nitrogen (N) availability is a strong determinant of plant biomass partitioning, but the role of different N sources in this process is unknown. Plants inhabiting low productivity ecosystems typically partition a large share of total biomass to belowground structures. In these systems, organic N may often dominate plant available N. With increasing productivity, plant biomass partitioning shifts to aboveground structures, along with a shift in available N to inorganic forms of N. We tested the hypothesis that the form of N taken up by plants is an important determinant of plant biomass partitioning by cultivating Arabidopsis thaliana on different N source mixtures. Plants grown on different N mixtures were similar in size, but those supplied with organic N displayed a significantly greater root fraction. (15)N labelling suggested that, in this case, a larger share of absorbed organic N was retained in roots and split-root experiments suggested this may depend on a direct incorporation of absorbed amino acid N into roots. These results suggest the form of N acquired affects plant biomass partitioning and adds new information on the interaction between N and biomass partitioning in plants.
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| 2. |
- Carlsson, Johanna, et al.
(författare)
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Nitrogen uptake and assimilation in proliferating embryogenic cultures of Norway spruce-Investigating the specific role of glutamine
- 2017
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Somatic embryogenesis is an in vitro system employed for plant propagation and the study of embryo development. Nitrogen is essential for plant growth and development and, hence, the production of healthy embryos during somatic embryogenesis. Glutamine has been shown to increase plant biomass in many in vitro applications, including somatic embryogenesis. However, several aspects of nitrogen nutrition during somatic embryogenesis remain unclear. Therefore, we investigated the uptake and assimilation of nitrogen in Norway spruce pro-embryogenic masses to elucidate some of these aspects. In our study, addition of glutamine had a more positive effect on growth than inorganic nitrogen. The nitrogen uptake appeared to be regulated, with a strong preference for glutamine; 67% of the assimilated nitrogen in the free amino acid pool originated from glutamine-nitrogen. Glutamine addition also relieved the apparently limited metabolism (as evidenced by the low concentration of free amino acids) of pro-embryogenic masses grown on inorganic nitrogen only. The unusually high alanine concentration in the presence of glutamine, suggests that alanine biosynthesis was involved in alleviating these constraints. These findings inspire further studies of nitrogen nutrition during the somatic embryogenesis process; identifying the mechanism(s) that govern glutamine enhancement of pro-embryogenic masses growth is especially important in this regard.
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| 3. |
- Carlsson, Johanna, et al.
(författare)
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Nitrogen utilization during germination of somatic embryos of Norway spruce : revealing the importance of supplied glutamine for nitrogen metabolism
- 2019
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Ingår i: Trees. - : SPRINGER HEIDELBERG. - 0931-1890 .- 1432-2285. ; 33:2, s. 383-394
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Tidskriftsartikel (refereegranskat)abstract
- Key messageThis paper shows that germinating Norway spruce somatic embryos are dependent on the carbon and nitrogen supplied in the medium, and that supplied glutamine accounts for 50 % of assimilated nitrogen during germination.AbstractThe female megagametophyte, which provides the zygotic embryo with nitrogen (N), carbon (C) and energy during germination, is not present in Norway spruce (Picea abies) mature somatic embryos. Therefore, somatic embryos presumably rely on nutrients supplied in the germination medium in addition to their storage compounds accumulated during maturation. However, to what extent stored versus supplied compounds contribute to a somatic embryo germination is unclear. In this 24-day study, we addressed the above question by monitoring the biomass changes and the N and C budget during somatic embryo germination, under low-intensity red light. We found that the C and N storage reserves, accumulated during the maturation phase, were not sufficient to support the growth of the germinating somatic embryos, rather they were dependent on the medium components. In addition, in a previous study it has been found that glutamine (Gln) supplied in the medium was crucial for maintaining the primary amino acid (AA) metabolism and growth of the proliferating embryogenic cultures of Norway spruce (Carlsson et al., PLoS One 12(8):e0181785, 2017). Therefore, we hypothesised that Gln would be required as a significant source of N also during somatic embryo germination. By tracing the uptake of isotopically labelled N-sources from the medium and further into primary N assimilation, we found that Gln was the preferred source of N for the germinating somatic embryos, accounting for 50% of assimilated N. As the amounts of both arginine (Arg) and Gln were increased in the germinating somatic embryos, it also suggested that germination in low-intensity red light promoted N storage, similar to what has been observed in the zygotic embryo maturation in conifers (King, Gifford, Plant Physiol 113:1125-1135, 1997).
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| 4. |
- Ganeteg, Ulrika, et al.
(författare)
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Amino acid transporter mutants of Arabidopsis provides evidence that a non-mycorrhizal plant acquires organic nitrogen from agricultural soil
- 2017
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Ingår i: Plant, Cell and Environment. - : Wiley. - 0140-7791 .- 1365-3040. ; 40, s. 413-423
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Tidskriftsartikel (refereegranskat)abstract
- Although organic nitrogen (N) compounds are ubiquitous in soil solutions, their potential role in plant N nutrition has been questioned. We performed a range of experiments on Arabidopsis thaliana genetically modified to enhance or reduce root uptake of amino acids. Plants lacking expression of the Lysine Histidine Transporter 1 (LHT1) displayed significantly lower contents of C-13 and N-15 label and of U-C-13(5),N-15(2) L-glutamine, as determined by liquid chromatography-mass spectrometry when growing in pots and supplied with dually labelled L-glutamine compared to wild type plants and LHT1-overexpressing plants. Slopes of regressions between accumulation of C-13-labelled carbon and N-15-labelled N were higher for LHT1-overexpressing plants than wild type plants, while plants lacking expression of LHT1 did not display a significant regression between the two isotopes. Uptake of labelled organic N from soil tallied with that of labelled ammonium for wild type plants and LHT1-overexpressing plants but was significantly lower for plants lacking expression of LHT1. When grown on agricultural soil plants lacking expression of LHT1 had the lowest, and plants overexpressing LHT1 the highest C/N ratios and natural N-15 abundance suggesting their dependence on different N pools. Our data show that LHT1 expression is crucial for plant uptake of organic N from soil.Brief Summary We studied the potential role of organic nitrogen (N) for plant N nutrition by feeding dual-labelled glutamine to soil-grown Arabidopsis thaliana mutants with enhanced or impeded expression of the amino-acid transporter LHT1. Significant differences between the genotypes in root contents of labelled glutamine and of N-15 and C-13 validate that it is the glutamine per se that is taken up by the root and not some product derived from it by microbial activity. Our results demonstrate that a non-mycorrhizal plant accesses organic N in competition with soil microbes and that expression of root organic N transporters is decisive for the efficacy of this process.
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| 5. |
- Gratz, Regina, et al.
(författare)
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Organic nitrogen nutrition: LHT1.2 protein from hybrid aspen (Populus tremula L. x tremuloides Michx) is a functional amino acid transporter and a homolog of Arabidopsis LHT1
- 2021
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Ingår i: Tree Physiology. - : Oxford University Press (OUP). - 0829-318X .- 1758-4469. ; 41, s. 1479–1496-
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Tidskriftsartikel (refereegranskat)abstract
- The contribution of amino acids (AAs) to soil nitrogen (N) fluxes is higher than previously thought. The fact that AA uptake is pivotal for N nutrition in boreal ecosystems highlights plant AA transporters as key components of the N cycle. At the same time, very little is known about AA transport and respective transporters in trees. Tree genomes may contain 13 or more genes encoding the lysine histidine transporter (LHT) family proteins, and this complicates the study of their significance for tree N-use efficiency. With the strategy of obtaining a tool to study N-use efficiency, our aim was to identify and characterize a relevant AA transporter in hybrid aspen (Populus tremula L. x tremuloides Michx.). We identified PtrLHT1.2, the closest homolog of Arabidopsis thaliana (L.) Heynh AtLHT1, which is expressed in leaves, stems and roots. Complementation of a yeast AA uptake mutant verified the function of PtrLHT1.2 as an AA transporter. Furthermore, PtrLHT1.2 was able to fully complement the phenotypes of the Arabidopsis AA uptake mutant lht1 aap5, including early leaf senescence-like phenotype, reduced growth, decreased plant N levels and reduced root AA uptake. Amino acid uptake studies finally showed that PtrLHT1.2 is a high affinity transporter for neutral and acidic AAs. Thus, we identified a functional AtLHT1 homolog in hybrid aspen, which harbors the potential to enhance overall plant N levels and hence increase biomass production. This finding provides a valuable tool for N nutrition studies in trees and opens new avenues to optimizing tree N-use efficiency.
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| 6. |
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| 7. |
- Hyll, Kari, et al.
(författare)
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CT-skanning som verktyg för detektering av törskateangrepp på tall
- 2022
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Rapport (refereegranskat)abstract
- Scots pine blister rust fungi (Cronartium pini) is expected to become an increasing problem for both forest owners and sawmills in the Nordic region, and there is great uncertainty about the economic consequences. There is also a lack of knowledge about the biology of Scots pine blister rust, as well as the tree's response and defence against ongoing infection. The tree is known to defend itself by enriching resin at the infected area, creating a damaged area of resin-wood. There is currently no reliable way to detect and assess the degree of damage in sawn timber, which leads to waste, as entire sawlogs are usually downgraded when parts or most of the timber could possibly be used. The objectives of this preliminary study were to: (1) investigate whether X-ray computed tomography (CT scanning) can detect blister rust damage in pine timber, in order to optimise sawing and save the value of the sawn timber; (2) to compare the 3-dimensional CT image description of the fungal attack with how the damage looks on the outside, to enable recommendations for external assessment of the damage attack, for example during felling; and (3) to assess the possibilities for describing the course of the damage and the tree's defence against the fungal attack.Eight rust-infested and two non-infested trees (Scots pine) were collected from a thinning stand and a mature stand outside Bjurselet, Norsjö municipality in Västerbotten. The trees were felled in January 2022. After felling, the stems were visually inspected, and infected parts were marked with paint on the mantle surface. The stems were cut into logs of suitable length for transport and scanning. Cuts through fungal-infested regions were avoided. The wood was transported to LTU’s Wood Science and Engineering facility in Skellefteå for CT scanning.The CT scan of the sample trees showed that damage due to pine blister rust can be detected in a way that enables further development of an industrial detection method and optimisation of the wood decomposition. However, one difficulty discovered was that fungal-infested sapwood areas have similar image intensity as non-infested heartwood. It could also be established that the distribution of damage inside the volume of the stem is greater than can be detected visually on the mantle, especially in the longitudinal direction. In this study the temporal development of the infection from the time of infestation to the time of CT scanning could not be analysed. However, annual rings could be distinguished in both damaged and undamaged wood, making it possible to monitor the evolution of the damage from year to year. DNA analysis detected the presence of blister rust fungus in the wood, both in and outside resin-rich areas. The highest amount of fungus-specific DNA was measured outside the resin-rich area in the outer parts of the sapwood.
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| 8. |
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| 9. |
- Svennerstam, Henrik, et al.
(författare)
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Comprehensive screening of Arabidopsis mutants suggests the lysine histidine transporter 1 to be involved in plant uptake of amino acids
- 2007
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Ingår i: Plant Physiology. - : Oxford University Press (OUP). - 1532-2548. ; 143:4, s. 1853-1860
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Tidskriftsartikel (refereegranskat)abstract
- Plant nitrogen (N) uptake is a key process in the global N cycle and is usually considered a "bottleneck” for biomass production in land ecosystems. Earlier, mineral N was considered the only form available to plants. Recent studies have questioned this dogma and shown that plants may access organic N sources such as amino acids. The actual mechanism enabling plants to access amino acid N is still unknown. However, a recent study suggested the Lysine Histidine Transporter 1 (LHT1) to be involved in root amino acid uptake. In this study, we isolated mutants defective in root amino acid uptake by screening Arabidopsis ( Arabidopsis thaliana) seeds from ethyl methanesulfonate-treated plants and seeds from amino acid transporter T-DNA knockout mutants for resistance against the toxic D-enantiomer of alanine ( Ala). Both ethyl methanesulfonate and T-DNA knockout plants identified as D-Ala resistant were found to be mutated in the LHT1 gene. LHT1 mutants displayed impaired capacity for uptake of a range of amino acids from solutions, displayed impaired growth when N was supplied in organic forms, and acquired substantially lower amounts of amino acids than wild-type plants from solid growth media. LHT1 mutants grown on mineral N did not display a phenotype until at the stage of flowering, when premature senescence of old leaf pairs occurred, suggesting that LHT1 may fulfill an important function at this developmental stage. Based on the broad and unbiased screening of mutants resistant to D-Ala, we suggest that LHT1 is an important mediator of root uptake of amino acids. This provides a molecular background for plant acquisition of organic N from the soil., ISSN = 0032-0889, DOI = 10.1104/pp.106.092205, url = ://WOS:000245781000034, year = 2007, type = Journal Article
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| 10. |
- Svennerstam, Henrik, et al.
(författare)
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Timing is everything - obtaining accurate measures of plant uptake of amino acids
- 2022
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Ingår i: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 234, s. 311-318
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Tidskriftsartikel (refereegranskat)abstract
- Plants are known to have the capacity to take up and utilise amino acids for growth. The significance of this uptake, however, remains elusive, partly due to methodological challenges and biological implications associated with acquiring and interpreting data. This study compared bulk stable isotope analysis and compound-specific liquid chromatography-mass spectrometry, two established methods for determining amino acid uptake. Root amino acid uptake was assayed using U-C-13(5)-N-15(2)-l-glutamine and axenically grown Arabidopsis thaliana. After 15-120 min of exposure, the content of intact glutamine measured in the roots was constant, whilst the N-15 and C-13 content increased over time, resulting in very different estimated uptake rates. The C-13 : N-15 ratio in roots declined with time, suggesting a loss of glutamine carbon of up to 15% within 120 min. The results presented indicate that, regardless of method used, time is a crucial factor when determining plant amino acid uptake. Due to post-uptake metabolism, compound-specific methods should primarily be used in experiments with a time frame of minutes rather than hours or days. Post-uptake metabolism in plants may account for significant loss of carbon, suggesting that it is not just pre-uptake metabolism by microbes that accounts for the N-15-C-13 mismatch reported in ecological studies, but also post-uptake metabolism in the plant.
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