| 1. |
- Madeja, Z, et al.
(författare)
-
New cationic polyprenyl derivative proposed as a lipofecting agent
- 2007
-
Ingår i: Acta biochimica Polonica. - : Polskie Towarzystwo Biochemiczne (Polish Biochemical Society). - 0001-527X .- 1734-154X. ; 54, s. 873-876
-
Tidskriftsartikel (refereegranskat)abstract
- Cationic linear poly-cis-isoprenoid prepared from natural plant polyprenol in a mixture with dioleyl phosphatidylethanolamine was found to be an effective lipofection agent for eukaryotic cells. The transfecting activity is related to the poly-cis structure of the polyprenyl chain.
|
|
| 2. |
|
|
| 3. |
- Carlson, Tomas, et al.
(författare)
-
Single polyprenol and dolichol isolation by semipreparative high-performance liquid chromatography technique
- 2000
-
Ingår i: Journal of Lipid Research. - 0022-2275 .- 1539-7262. ; 41:7, s. 1177-1180
-
Tidskriftsartikel (refereegranskat)abstract
- A new method of separation of single polyprenols (or dolichols) from a mixture of isoprenoid alcohols is described. Application of a high-performance liquid chromatography (HPLC) apparatus equipped with a semipreparative ODS column resulted in preparation of long-chain (dihydro)polyprenols of high purity (>95%). This approach substantially decreases the time scale of the conventional chromatographical preparative procedure. The method can be widely used in chemical and biochemical projects, where single polyprenols or dolichols are required.
|
|
| 4. |
- Dallner, G., et al.
(författare)
-
Dehydro-Tocotrienol-beta Counteracts Oxidative-Stress-Induced Diabetes Complications in db/db Mice
- 2021
-
Ingår i: Antioxidants. - : MDPI AG. - 2076-3921. ; 10:7
-
Tidskriftsartikel (refereegranskat)abstract
- Hyperglycemia, hyperlipidemia, and adiposity are the main factors that cause inflammation in type 2 diabetes due to excessive ROS production, leading to late complications. To counteract the effects of increased free radical production, we searched for a compound with effective antioxidant properties that can induce coenzyme Q biosynthesis without affecting normal cellular functions. Tocotrienols are members of the vitamin E family, well-known as efficient antioxidants that are more effective than tocopherols. Deh-T3 beta is a modified form of the naturally occurring tocotrienol-beta. The synthesis of this compound involves the sequential modification of geranylgeraniol. In this study, we investigated the effects of this compound in different experimental models of diabetes complications. Deh-T3 beta was found to possess multifaceted capacities. In addition to enhanced wound healing, deh-T3 beta improved kidney and liver functions, reduced liver steatosis, and improved heart recovery after ischemia and insulin sensitivity in adipose tissue in a mice model of type 2 diabetes. Deh-T3 beta exerts these positive effects in several organs of the diabetic mice without reducing the non-fasting blood glucose levels, suggesting that both its antioxidant properties and improvement in mitochondrial function are involved, which are central to reducing diabetes complications.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
- Parmryd, Ingela, et al.
(författare)
-
Chloroplastic prenylated proteins
- 1997
-
Ingår i: FEBS Letters. - 0014-5793 .- 1873-3468. ; 414:3, s. 527-531
-
Tidskriftsartikel (refereegranskat)abstract
- By in vivo [3H]mevalonate labelling of spinach combined with biochemical analysis, evidence is provided for the existence of protein prenylation in chloroplasts. Approximately 20 prenylated polypeptides were resolved by SDS-PAGE followed by autoradiography. Thermolysin treatment of intact chloroplasts revealed that about 40% of the prenylated polypeptides were associated with the cytoplasmic surface of the outer envelope membrane. The remaining portion was present in thylakoids and/or the inner envelope membrane. The majority of the prenylated polypeptides were associated with larger membrane protein complexes. A farnesyl protein transferase activity was found to be associated with the thylakoid membrane.
|
|
| 9. |
- Parmryd, Ingela, et al.
(författare)
-
Identification of spinach farnesyl protein transferase : Dithiothreitol as an acceptor in vitro
- 1995
-
Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 234:3, s. 723-731
-
Tidskriftsartikel (refereegranskat)abstract
- Spinach seedlings were found to contain farnesyl protein transferase. The enzyme is activated by Zn2+, but not by Mg2+. The pH optimum is approximately 7.0 and maximal activity is obtained at 40-45 degrees C. The apparent Km for the farnesyl diphosphate substrate is 7 microM. Western blotting of soluble proteins with an antiserum raised against mammalian farnesyl protein transferase demonstrated a specific cross-reactivity with the spinach enzyme. The antiserum preferentially recognises the beta-subunit of the heterodimeric farnesyl protein transferase, and the corresponding spinach polypeptide has a molecular mass of 42 kDa on SDS/PAGE. The enzyme can employ dithiothreitol as an acceptor for the farnesyl moiety and catalyses the formation of a thioether linkage between these substrates. On the basis of this discovery, a new method was developed utilising the hydrophobicity of the reaction product, and its interaction with poly(propylene). During in vivo labelling, the plants took up dithiothreitol, which inhibited the incorporation of [3H]mevalonate metabolites into proteins, indicating that dithiothreitol might be isoprenylated in vivo as well as in vitro. However, isoprenylation of some proteins remains unaffected by dithiothreitol suggesting the existence of different isoprenylation mechanisms. Thus, it is demonstrated that plants possess farnesyl protein transferase, which resembles its mammalian and yeast homologues.
|
|
| 10. |
- Shipton, C. A., et al.
(författare)
-
Isoprenylation of plant proteins in vivo : Isoprenylated proteins are abundant in the mitochondria and nuclei of spinach
- 1995
-
Ingår i: Journal of Biological Chemistry. - : Elsevier BV. - 0021-9258 .- 1083-351X. ; 270:2, s. 566-572
-
Tidskriftsartikel (refereegranskat)abstract
- Protein isoprenylation in vivo is demonstrated using spinach seedlings labeled with [3H]mevalonate. This report provides evidence for the occurrence of a large number of isoprenylated proteins in plants. Seedlings, without roots, were labeled quantitatively through the cut stem. Mevinolin treatment of the seedlings resulted in increased incorporation of radiolabel into proteins. Approximately 30 labeled bands could be detected after autoradiography of SDS-polyacrylamide gel electrophoresis-separated polypeptides, ranging in molecular mass from 6 to 200 kDa. Methyl iodide hydrolysis resulted in the release of covalently bound farnesol, geranylgeraniol, phytol, and some unidentified isoprenoid compounds from mevalonate-labeled proteins. It was found that all cellular fractions contained some isoprenylated proteins, although most were located in the mitochondria and nuclei. Subfractionation of the nucleus revealed that the majority of isoprenylated proteins in this compartment were components of the nuclear matrix. The results demonstrate that in vivo labeling of a complex organism can be performed using a plant system in order to study protein isoprenylation and distribution of modified proteins in different cellular compartments.
|
|
