| 1. |
- Bartosiewicz, Laszlo, 1954-, et al.
(författare)
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Animal Exploitation in Medieval Hungary
- 2018
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Ingår i: The Economy of Medieval Hungary. - Leiden : Brill Academic Publishers. - 9789004310155 ; , s. 113-165
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Bokkapitel (refereegranskat)abstract
- During the last few years there has been an upswing in interest in animal stud-ies among medievalists. Historical research into medieval animal husbandry and the use of related products has intensified in terms of the analysis of docu-mentary (and to some extent, iconographic) sources, but the help of archae-ologists has also been enlisted. Eventually, the study of animal bone finds also began, although this type of inquiry is far better developed in the field of pre-historic archaeology: in the absence of written sources, prehistorians had to turn to less spectacular evidence, including animal remains. Archaeozoology is devoted to the identification, analysis and interpretation of animal remains from archaeological sites. Although the detailed analysis of written sources and animal iconography fall outside the scope of archaeozoology, familiarity with this process is indispensable for properly interpreting the archaeological traces of medieval animal exploitation.
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| 2. |
- Giesecke, Marianne, et al.
(författare)
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The protonation state and binding mode in a metal coordination complex from the charge measured in solution by electrophoretic NMR
- 2013
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Ingår i: Analytical Methods. - : Royal Society of Chemistry (RSC). - 1759-9660 .- 1759-9679. ; 5:7, s. 1648-1651
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Tidskriftsartikel (refereegranskat)abstract
- We measured with high accuracy the effective charge of a uranium (VI)-AMP complex by electrophoretic NMR (eNMR). Using the same method, the degree of counterion association is also assessed which leads to a quantitative determination of the nominal charge which then provides the degree of ligand deprotonation in the complex. This demonstrates a new application of eNMR for resolving structural details of supramolecular complexes.
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| 3. |
- Henriksson, Gunnar, et al.
(författare)
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Substrate specificity of cellobiose dehydrogenase from Phanerochaete chrysosporium
- 1998
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Ingår i: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 1383:1, s. 48-54
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Tidskriftsartikel (refereegranskat)abstract
- Substrate structural mapping suggests that the catalytic site of cellobiose dehydrogenase from Phanerochaete chrysosporium forms a narrow cave with two hexose binding subsites, Kinetic data also show that beta-di or oligosaccharides are favored electron donors with respect to both KM and kcat. Surprisingly, thiocellobiose showed an even higher kcat than cellobiose, although the KM value was somewhat higher. The CDH was purified using an updated protocol.
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| 4. |
- Johansson, L, et al.
(författare)
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Gasrening med regenererande aktiv-kol-system adsorptions- och desorptionsstudier i laboratorieskala
- 1984
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Adsorption av organiska föroreningar i luft till aktiverat kol med efterföljande desorption och återvinning av adsorbatet, är i många fall en tekniskt/ekonomiskt intressant reningsmetod. Tekniken används idag konventionellt i samband med återvinning av bl a tri-och perkloretylen och toluen. Möjligheterna att använda samma förfarande för att behandla processgasströmmar som innehåller mer högkokande ämnen har tidigare studerats genom fältförsök i pilotskala (IVL-rapport A737). Denna rapport redogör för de fortsatta försök av aktiv-kol teknikens möjligheter som genomförts i laboratorieskala. Resultaten visar att studium av adsorption- och desorptionsförloppen med genomströmningsreaktor i laboratorieskala kan ge värdefull information som kan ligga till grund för dimensionering av en fullskaleanläggning. Enbart adsorptionsisotermer utgör däremot inte ett användbart underlag. Möjligheterna att effektivisera desorptionen med hetgas vid olika absoluttryck eller genom mikrovågsvärmning har också studerats. Någon teknisk/ekonomisk bedömning av metoderna går dock inte att göra med erhållna resultat.
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| 5. |
- Johansson, Leif, et al.
(författare)
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Lösningsmedelshantering vid möbelindustri och träimpregnering.
- 1984
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Industrin i Sverige släpper ut c:a 160.000 ton lösningsmedel per år i form av processluft, fast och flytande avfall. Huvuddelen av detta utgörs av luftutsläpp. Miljövårdskrav och stigande råvarupriser har framtvingat en bättre slutning av processer, så att förluster av lösningsmedel succesivt har kunnat minskas. Reningstekniker såsom adsorption, absorption, biobädd och kondensering har visat att det är tekniskt/ekonomiskt möjligt att behandla lösningsmedelhaltiga luftutsläpp. Ett företag från vardera möbelindustrin respektive träimpregneringen har här utvalts för att belysa åtgärdsmöjligheter vid specifika branscher. Kostnaderna för extern luftreningsutrustning är starkt beroende av det luftflöde som ska behandlas. I denna studie har därför ett antal tänkta fall medtagits som innebär en viss processmodifiering och därmed reducering av emitterat luftflöde.
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| 6. |
- Leekitcharoenphon, Pimlapas, et al.
(författare)
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Cross-Border Transmission of Salmonella Choleraesuis var. Kunzendorf in European Pigs and Wild Boar : Infection, Genetics, and Evolution
- 2019
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Ingår i: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Salmonella enterica subspecies enterica serotype Choleraesuis is a swine adapted serovar. S. Choleraesuis variant Kunzendorf are responsible for the majority of outbreaks among pigs. S. Choleraesuis is rare in Europe, although there have been serious outbreaks in pigs including two outbreaks in Denmark in 1999-2000 and 2012-2013. Here, we elucidate the epidemiology, possible transmission routes and sources, and clonality of European S. Choleraesuis isolates including the Danish outbreak isolates. A total of 102 S. Choleraesuis isolates from different European countries and the United States of America, covering available isolates from the last two decades were selected for whole genome sequencing. We applied a temporally structured sequence analysis within a Bayesian framework to reconstruct a temporal and spatial phylogenetic tree. MLST type, resistance genes, plasmid replicons and accessory genes were identified using bioinformatics tools. Fifty-eight isolates including 11 out of 12 strains from wild boars were pan-susceptible. The remaining isolates carried multiple resistance genes. Eleven different plasmid replicons in eight plasmids were determined among the isolates. Accessory genes were associated to the identified resistance genes and plasmids. The European S. Choleraesuis was estimated to have emerged in 1837 (95% credible interval, 1733 - 1983) with the mutation rate of 1.02 SNPs/genome/year. The isolates were clustered according to countries and neighbour countries. There were transmission events between strains from the USA and European countries. Wild boar and pig isolates were genetically linked suggesting cross-border transmission and transmission due to a wildlife reservoir. The phylogenetic tree shows that multiple introductions were responsible for the outbreak of 2012-2013 in Denmark, and suggests that poorly disinfected vehicles crossing the border into Denmark were potentially the source of the outbreak. Low levels of single nucleotide polymorphisms (SNP) differences (0-4 SNPs) can be observed between clonal strains isolated from different organs of the same animal. Proper disinfection of livestock vehicles and improved quality control of livestock feed are recommended to prevent future spread of S. Choleraesuis or other more serious infectious diseases such as African swine fever (ASF) into the European pig production system.
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| 7. |
- Mareş, Răzvan Gheorghiţă, et al.
(författare)
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S100A8/A9 is a valuable biomarker and treatment target to detect and modulate neutrophil involvement in myocardial infarction
- 2023
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Ingår i: Romanian Journal of Morphology and Embryology. - 1220-0522. ; 64:2, s. 151-158
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Tidskriftsartikel (refereegranskat)abstract
- Myocardial infarction (MI) leads to irreversible ischemic damage of the heart muscle and is the leading cause of heart failure. The ischemic cardiac injury triggers a potent local and systemic immune response. In the acute phase post-MI, neutrophils infiltrate the myocardium in large numbers and induce further cardiomyocyte death, expanding the infarcted area. The alarmin S100A8/A9 is a proinflammatory mediator primarily produced by myeloid cells, with an emerging role in MI. We previously demonstrated that short-term inhibition of S100A8/A9 during the inflammatory phase of the immune response to MI improves long-term cardiac function. In the present study, we investigated the effects of S100A8/A9 blockade on myocardial inflammation and post-ischemic myocardial injury in a mouse model of coronary artery ligation. Immunohistochemical (IHC) staining revealed that the presence of S100A9 is strongly correlated with neutrophil infiltration in the myocardium on days 1 and 3 post-MI. A 3-day treatment with the S100A8/A9 blocker ABR-238901 starting immediately after MI decreased the number of neutrophils and S100A9 presence in the myocardium and had a positive impact on cardiac damage, reducing infarction size. These findings promote S100A9 as an IHC biomarker of neutrophil infiltration and a promising immunomodulatory target to regulate neutrophil recruitment, reduce ischemic injury and promote long-term beneficial cardiac recovery after MI.
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| 8. |
- Mares, Razvan Gheorghita, et al.
(författare)
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Studying the innate immune response to myocardial infarction in a highly efficient experimental animal model
- 2021
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Ingår i: Revista Romana de Cardiologie. - : Walter de Gruyter GmbH. - 1220-658X. ; 31:3, s. 573-585
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Tidskriftsartikel (refereegranskat)abstract
- The reduction in mortality following acute myocardial infarction (AMI) is an important achievement of modern medicine. Despite this progress, AMI remains the most common cause of heart failure (HF) and HF-related morbidity and mortality. The involvement of the innate immune response in different stages after AMI has attracted important attention in recent years. With the increasing range of potential therapeutic compounds and delivery vectors, the need of highly efficient experimental AMI models is increasing, to support further advancement in this field. Here, we present a high-throughput model for the assessment of the innate immune response to AMI. The model is based on permanent surgical ligation of the left descending coronary artery (LAD) in mice, followed by complex flow-cytometry and histological analyses of immune cellular populations in blood and myocardium. We are presenting time-dependent qualitative and quantitative analysis results, demonstrating intense accumulation of Ly6Ghi neutrophils and Ly6Chi monocytes in the infarcted myocardium on days 1 and 3 post-AMI, followed by successive accumulation of reparatory Ly6CloMerTKhi macrophages, neovascularization and fibrosis development by day 7.
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| 9. |
- Marinković, Goran, et al.
(författare)
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Inhibition of pro-inflammatory myeloid cell responses by short-term S100A9 blockade improves cardiac function after myocardial infarction
- 2019
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Ingår i: European Heart Journal. - : Oxford University Press (OUP). - 1522-9645 .- 0195-668X. ; 40:32, s. 2713-2723
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Tidskriftsartikel (refereegranskat)abstract
- AIMS: Neutrophils have both detrimental and beneficial effects in myocardial infarction (MI), but little is known about the underlying pathways. S100A8/A9 is a pro-inflammatory alarmin abundantly expressed in neutrophils that is rapidly released in the myocardium and circulation after myocardial ischaemia. We investigated the role of S100A8/A9 in the innate immune response to MI. METHODS AND RESULTS: In 524 patients with acute coronary syndrome (ACS), we found that high plasma S100A8/A9 at the time of the acute event was associated with lower left ventricular ejection fraction (EF) at 1-year and increased hospitalization for heart failure (HF) during follow-up. In wild-type C57BL/6 mice with MI induced by permanent coronary artery ligation, treatment with the S100A9 blocker ABR-238901 during the inflammatory phase of the immune response inhibited haematopoietic stem cell proliferation and myeloid cell egression from the bone marrow. The treatment reduced the numbers of neutrophils and monocytes/macrophages in the myocardium, promoted an anti-inflammatory environment, and significantly improved cardiac function compared with MI controls. To mimic the clinical scenario, we further confirmed the effects of the treatment in a mouse model of ischaemia/reperfusion. Compared with untreated mice, 3-day ABR-238901 treatment significantly improved left ventricular EF (48% vs. 35%, P = 0.002) and cardiac output (15.7 vs. 11.1 mL/min, P = 0.002) by Day 21 post-MI. CONCLUSION: Short-term S100A9 blockade inhibits inflammation and improves cardiac function in murine models of MI. As an excessive S100A8/A9 release is linked to incident HF, S100A9 blockade might represent a feasible strategy to improve prognosis in ACS patients.
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| 10. |
- Molnar, Miklos Z, et al.
(författare)
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Association of incident obstructive sleep apnoea with outcomes in a large cohort of US veterans
- 2015
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Ingår i: Thorax. - : BMJ. - 0040-6376 .- 1468-3296. ; 70:9, s. 888-895
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Tidskriftsartikel (refereegranskat)abstract
- RATIONALE: There is a paucity of large cohort studies examining the association of obstructive sleep apnoea (OSA) with clinical outcomes including all-cause mortality, coronary heart disease (CHD), strokes and chronic kidney disease (CKD).OBJECTIVES: We hypothesised that a diagnosis of incident OSA is associated with higher risks of these adverse clinical outcomes.METHODS, MEASUREMENTS: In a nationally representative cohort of over 3 million (n=3 079 514) US veterans (93% male) with baseline estimated glomerular filtration rate (eGFR)≥60 mL/min/1.73 m(2), we examined the association between the diagnosis of incident OSA, treated and untreated with CPAP, and: (1) all-cause mortality, (2) incident CHD, (3) incident strokes, (4)incident CKD defined as eGFR<60 mL/min/1.73 m(2), and (5) slopes of eGFR.MAIN RESULTS: Compared with OSA-negative patients, untreated and treated OSA was associated with 86% higher mortality risk, (adjusted HR and 95% CI 1.86 (1.81 to 1.91) and 35% (1.35 (1.21 to 1.51)), respectively. Similarly, untreated and treated OSA was associated with 3.5 times (3.54 (3.40 to 3.69)) and 3 times (3.06 (2.62 to 3.56)) higher risk of incident CHD; 3.5 times higher risk of incident strokes (3.48 (3.28 to 3.64) and 3.50 (2.92 to 4.19)) for untreated and treated OSA, respectively. The risk of incident CKD was also significantly higher in untreated (2.27 (2.19 to 2.36)) and treated (2.79 (2.48 to 3.13)) patients with OSA. The median (IQR) of the eGFR slope was -0.41 (-2.01 to 0.99), -0.61 (-2.69 to 0.93) and -0.87 (-3.00 to 0.70) mL/min/1.73 m(2) in OSA-negative patients, untreated OSA-positive patients and treated OSA-positive patients, respectively.CONCLUSIONS: In this large and contemporary cohort of more than 3 million US veterans, a diagnosis of incident OSA was associated with higher mortality, incident CHD, stroke and CKD and with faster kidney function decline.
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