| 1. |
- Ben-Menachem, Elinor, 1945, et al.
(författare)
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Long-term safety and tolerability of lacosamide monotherapy in patients with epilepsy: Results from a multicenter, open-label trial
- 2021
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Ingår i: Epilepsia Open. - : Wiley. - 2470-9239. ; 6:3
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Tidskriftsartikel (refereegranskat)abstract
- The primary objective of this trial (SP1042; NCT02582866) was to assess long-term safety and tolerability of lacosamide monotherapy (200-600 mg/day) in adults with focal (partial-onset) seizures or generalized tonic-clonic seizures (without clear focal origin). This Phase III, long-term, open-label, multicenter, follow-up trial enrolled patients with epilepsy who were taking lacosamide in, and completed, the previous double-blind trial (SP0994; NCT01465997). Primary safety outcomes were treatment-emergent adverse events (TEAEs), discontinuations due to TEAEs, and serious TEAEs. One hundred and six patients were enrolled and received lacosamide: 84 (79.2%) completed the trial and 22 (20.8%) discontinued. The median duration of exposure was 854.0 days, with a median modal dose of 200 mg/day. Ninety-six (90.6%), 64 (60.4%), and 44 (41.5%) patients had >= 12, >= 24, and >= 36 months of lacosamide exposure, respectively. At least one TEAE was reported by 61 (57.5%) patients. The most common (>= 4%) TEAEs were headache (10 [9.4%]), nasopharyngitis (eight [7.5%]), and back pain (five [4.7%]). One (0.9%) patient discontinued due to a TEAE (sudden unexpected death in epilepsy; not considered drug-related), 14 (13.2%) patients reported serious TEAEs, and seven (6.6%) patients reported TEAEs that were considered drug-related. Overall, long-term lacosamide monotherapy was generally well tolerated up to 600 mg/day, with no new safety signals identified.
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| 2. |
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| 3. |
- Al Sam, Ali, et al.
(författare)
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The effect of moving waves on neutral marine atmospheric boundary layer
- 2014
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Ingår i: First Symposium on OpenFOAM® in Wind Energy. - : EDP Sciences. ; 2:01003
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Konferensbidrag (refereegranskat)abstract
- Large eddy simulations are performed to study the effects of wind-wave direction misalignment of the neutral marine atmospheric boundary layer over a wavy wall. The results show that the wind-wave misalignment has a significant effect on the velocity profiles and the pressure fluctuation over the wave surface. These effects are not confined to the near wave surface region but extend over the whole atmospheric surface layer.
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| 4. |
- Almeida, Natália, et al.
(författare)
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Mapping the melanoma plasma proteome (MPP) using single-shot proteomics interfaced with the WiMT database
- 2021
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Ingår i: Cancers. - : MDPI AG. - 2072-6694. ; 13:24
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Tidskriftsartikel (refereegranskat)abstract
- Plasma analysis by mass spectrometry-based proteomics remains a challenge due to its large dynamic range of 10 orders in magnitude. We created a methodology for protein identification known as Wise MS Transfer (WiMT). Melanoma plasma samples from biobank archives were directly analyzed using simple sample preparation. WiMT is based on MS1 features between several MS runs together with custom protein databases for ID generation. This entails a multi-level dynamic protein database with different immunodepletion strategies by applying single-shot proteomics. The highest number of melanoma plasma proteins from undepleted and unfractionated plasma was reported, mapping >1200 proteins from >10,000 protein sequences with confirmed significance scoring. Of these, more than 660 proteins were annotated by WiMT from the resulting ~5800 protein sequences. We could verify 4000 proteins by MS1t analysis from HeLA extracts. The WiMT platform provided an output in which 12 previously well-known candidate markers were identified. We also identified low-abundant proteins with functions related to (i) cell signaling, (ii) immune system regulators, and (iii) proteins regulating folding, sorting, and degradation, as well as (iv) vesicular transport proteins. WiMT holds the potential for use in large-scale screening studies with simple sample preparation, and can lead to the discovery of novel proteins with key melanoma disease functions.
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| 5. |
- Betancourt, Lazaro Hiram, et al.
(författare)
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The human melanoma proteome atlas-Defining the molecular pathology
- 2021
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Ingår i: Clinical and Translational Medicine. - : Wiley. - 2001-1326. ; 11:7, s. 1-20
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Tidskriftsartikel (refereegranskat)abstract
- The MM500 study is an initiative to map the protein levels in malignant melanoma tumor samples, focused on in-depth histopathology coupled to proteome characterization. The protein levels and localization were determined for a broad spectrum of diverse, surgically isolated melanoma tumors originating from multiple body locations. More than 15,500 proteoforms were identified by mass spectrometry, from which chromosomal and subcellular localization was annotated within both primary and metastatic melanoma. The data generated by global proteomic experiments covered 72% of the proteins identified in the recently reported high stringency blueprint of the human proteome. This study contributes to the NIH Cancer Moonshot initiative combining detailed histopathological presentation with the molecular characterization for 505 melanoma tumor samples, localized in 26 organs from 232 patients.
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| 6. |
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| 7. |
- Denes, L, et al.
(författare)
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In Situ Hybridization of PRRSV-1 Combined with Digital Image Analysis in Lung Tissues of Pigs Challenged with PRRSV-1
- 2021
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Ingår i: Veterinary sciences. - : MDPI AG. - 2306-7381. ; 8:10
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Tidskriftsartikel (refereegranskat)abstract
- Betaarterivirus suid 1 and 2 are the causative agents of porcine reproductive and respiratory syndrome (PRRS), which is one of the most significant diseases of the swine industry, causing significant economic losses in the main pig producing countries. Here, we report the development of a novel, RNA-based in situ hybridization technique (RNAscope) to detect PRRS virus (PRRSV) RNA in lung tissues of experimentally infected animals. The technique was applied to lung tissues of 20 piglets, which had been inoculated with a wild-type, highly pathogenic PRRSV-1 strain. To determine the RNAscope’s applicability as a semi-quantitative method, we analysed the association between the proportion of the virus-infected cells measured with an image analysis software (QuPath) and the outcome of the real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) tests performed in parallel. The results of the quantitative approach of these two molecular biological methods show significant association (pseudo R2 = 0.3894, p = 0.004). This is the first time RNAscope assay has been implemented for the detection of PRRSV-1 in experimental animals.
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| 8. |
- Duwig, Christophe, et al.
(författare)
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Large Eddy simulation of a swirling flame response to swirl modulation with impact on combustion stability
- 2005
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Ingår i: 43rd AIAA Aerospace Sciences Meeting and Exhibit - Meeting Papers. ; , s. 4929-4940
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Konferensbidrag (refereegranskat)abstract
- A swirl stabilized flame has been studied numerically using Large Eddy Simulation. The response of the flame to a large swirl number modulation has as been determined. Decreasing the swirl number moderately does not destabilize the flame. Decreasing further the swirl number induces a transition from stabilized to a jet flame with increase in the flame fluctuations. Increasing back the swirl restores the original configuration. However, the re-stabilization has been found to be considerably slower as compared to the burner aerodynamic time scale. Further studies, including identification of some characteristic turbulent structures, have been carried out. Both a helical and an axial mode have been identified. The helical mode arises in the central recirculation zone, whereas the axial mode arises in the outer shear-layer. Both modes are possible sources of thermo-acoustic instabilities.
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| 9. |
- Ivády, Viktor, et al.
(författare)
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High-Fidelity Bidirectional Nuclear Qubit Initialization in SiC
- 2016
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Ingår i: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 117:22
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Tidskriftsartikel (refereegranskat)abstract
- Dynamic nuclear polarization (DNP) is an attractive method for initializing nuclear spins that are strongly coupled to optically active electron spins because it functions at room temperature and does not require strong magnetic fields. In this Letter, we theoretically demonstrate that DNP, with near-unity polarization efficiency, can be generally realized in weakly coupled electron spin-nuclear spin systems. Furthermore, we theoretically and experimentally show that the nuclear spin polarization can be reversed by magnetic field variations as small as 0.8 Gauss. This mechanism offers new avenues for DNP-based sensors and radio-frequency free control of nuclear qubits.
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| 10. |
- Kero, J., et al.
(författare)
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MU radar head echo observations of the 2011 October Draconids
- 2012
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Ingår i: Monthly notices of the Royal Astronomical Society. - Hoboken, NJ : Wiley-Blackwell. - 0035-8711 .- 1365-2966. ; 424:3, s. 1799-1806
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Tidskriftsartikel (refereegranskat)abstract
- On 2011 October 8, the Earth passed through a stream of dust ejected by the comet 21P/GiacobiniZinner during its perihelion passage of the year 1900, causing an outburst of October Draconid meteors. 13 Draconids were observed among similar to 6300 meteor head echoes with precisely determined orbits during an observational campaign ranging from October 8 05:00 ut to October 9 13:00 ut with the Shigaraki middle and upper atmosphere (MU) radar in Japan (34 degrees.85?N and 136 degrees.10?E). The meteor outburst occurred while the Draconid radiant was descending below and 2?h later rising up above the horizon. Therefore, 11 of the detections were from very low (<15 degrees) elevation. The detection altitudes of the Draconids were high compared to sporadic meteors of the same velocity and radiant elevation. The weighted mean geocentric velocity of the 13 Draconids was 20.6 +/- 0.4?km?s-1, and the weighted mean radiant located at right ascension alpha = 263 degrees.3 +/- 0 degrees.6 and declination delta = 55 degrees.8 +/- 0 degrees.2.
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