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| 2. |
- Aad, G, et al.
(författare)
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- 2015
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swepub:Mat__t
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| 3. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 4. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 5. |
- Fam, Mei Ling, et al.
(författare)
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Data learning and expert judgment in a bayesian belief network for offshore decommissioning risk assessment
- 2018
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Ingår i: Safety and Reliability - Safe Societies in a Changing World. - : CRC Press/Balkema. ; , s. 397-406
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Konferensbidrag (refereegranskat)abstract
- Decommissioning of offshore facilities involve changing risk profiles at different decommissioning phases. Bayesian Belief networks (BBNs) are used as part of the proposed risk assessment method to capture the multiple interactions of a decommissioning activity. The Bayesian Belief network is structured from the data learning of an accident database and a modification of the BBN nodes to incorporate human factors and barrier performance modelling. The analysis covers one case study of one area of decommissioning operations by extrapolating well workover data to well plugging and abandonment. Initial analysis from well workover data, of a 5-node BBN provided insights on two different levels of severity of an accident, the “Accident” and “Incident” level, and on its respective profiles of the initiating events and the investigation-reported human causes. The initial results demonstrate that the data learnt from the database can be used to structure the BBN, and give insights on how human factors pertaining to well activities can be modelled, and that the relative frequencies can act as initial data input for the proposed nodes. It is also proposed that the integrated treatment of various sources of information (database and expert judgement) through a BBN model can support the risk assessment of a dynamic situation such as offshore decommissioning.
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| 6. |
- Hein, Sascha, et al.
(författare)
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Psychological and Sociocultural Adaptation of Children Adopted from Russia and their Associations with Pre-Adoption Risk Factors and Parenting
- 2017
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Ingår i: Journal of Child and Family Studies. - : SPRINGER. - 1062-1024 .- 1573-2843. ; 26:10, s. 2669-2680
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Tidskriftsartikel (refereegranskat)abstract
- We investigated academic and behavioral outcomes of internationally adopted children and the associations between these outcomes and age at adoption, time spent in the adoptive family, and parenting. At two time points (T1 and T2, similar to 15 months apart), we examined early academic skills (school readiness), and parent-reported behavioral adjustment (internalizing and externalizing behavior) and adaptive functioning of a sample of 75 children (45.9% boys, mean age = 5.17 years) adopted from Russia into US families. We also collected parents' self-assessments of their parenting at T1. Children who were adopted at a younger age showed higher levels of early academic skills. Correlations between age at adoption and other outcomes were overall small and mostly non-significant. However, adoptees' academic and behavioral progress differed notably in several respects. Specifically, adoptees improved in early academic skills over time, whereas, as a group, their adaptive functioning and behavioral adjustment remained stable within the normal range. Early academic skills were not related to behavioral adjustment at each time point and over time. The time spent in the adoptive family was positively related to early academic skills at T2. Whereas outcomes showed little to no relation to parenting as reported by mother and father separately, higher discrepancies between mothers' and fathers' reports of positive parenting were related to higher levels of behavioral symptoms and lower levels of adaptive skills at T2. These differential results may be explained in part by drawing upon the notion of dissociated domains of psychological and sociocultural adaptation and acculturation, outlined in the immigration literature. These results also bring to light the possible importance of between-parent consistency in parenting for adoptees' behavioral outcomes.
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| 7. |
- Hsu, Pohao, et al.
(författare)
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Extremely Low-light Image Enhancement with Scene Text Restoration
- 2022
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Ingår i: Proceedings - International Conference on Pattern Recognition. - 1051-4651. - 9781665490627 ; 2022-August, s. 317-323
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Konferensbidrag (refereegranskat)abstract
- Deep learning based methods have made impressive progress in enhancing extremely low-light images - the image quality of the reconstructed images has generally improved. However, we found out that most of these methods could not sufficiently recover the image details, for instance the texts in the scene. In this paper, a novel image enhancement framework is proposed to specifically restore the scene texts, as well as the overall quality of the image simultaneously under extremely low-light images conditions. Particularly, we employed a selfregularised attention map, an edge map, and a novel text detection loss. The quantitative and qualitative experimental results have shown that the proposed model outperforms stateof-the-art methods in terms of image restoration, text detection, and text spotting on See In the Dark and ICDAR15 datasets.
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