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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 5. |
- Fenstermacher, M.E., et al.
(författare)
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DIII-D research advancing the physics basis for optimizing the tokamak approach to fusion energy
- 2022
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 0029-5515 .- 1741-4326. ; 62:4
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Tidskriftsartikel (refereegranskat)abstract
- DIII-D physics research addresses critical challenges for the operation of ITER and the next generation of fusion energy devices. This is done through a focus on innovations to provide solutions for high performance long pulse operation, coupled with fundamental plasma physics understanding and model validation, to drive scenario development by integrating high performance core and boundary plasmas. Substantial increases in off-axis current drive efficiency from an innovative top launch system for EC power, and in pressure broadening for Alfven eigenmode control from a co-/counter-I p steerable off-axis neutral beam, all improve the prospects for optimization of future long pulse/steady state high performance tokamak operation. Fundamental studies into the modes that drive the evolution of the pedestal pressure profile and electron vs ion heat flux validate predictive models of pedestal recovery after ELMs. Understanding the physics mechanisms of ELM control and density pumpout by 3D magnetic perturbation fields leads to confident predictions for ITER and future devices. Validated modeling of high-Z shattered pellet injection for disruption mitigation, runaway electron dissipation, and techniques for disruption prediction and avoidance including machine learning, give confidence in handling disruptivity for future devices. For the non-nuclear phase of ITER, two actuators are identified to lower the L-H threshold power in hydrogen plasmas. With this physics understanding and suite of capabilities, a high poloidal beta optimized-core scenario with an internal transport barrier that projects nearly to Q = 10 in ITER at ∼8 MA was coupled to a detached divertor, and a near super H-mode optimized-pedestal scenario with co-I p beam injection was coupled to a radiative divertor. The hybrid core scenario was achieved directly, without the need for anomalous current diffusion, using off-axis current drive actuators. Also, a controller to assess proximity to stability limits and regulate β N in the ITER baseline scenario, based on plasma response to probing 3D fields, was demonstrated. Finally, innovative tokamak operation using a negative triangularity shape showed many attractive features for future pilot plant operation.
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- Qin, Tao, 1984-, et al.
(författare)
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A novel method to synthesize low-cost magnesium fluoride spheres from seawater
- 2017
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Ingår i: Ceramics International. - : ELSEVIER SCI LTD. - 0272-8842 .- 1873-3956. ; 43:16, s. 14481-14483
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Tidskriftsartikel (refereegranskat)abstract
- Magnesium fluoride (MgF2) is widely used as optical materials. A novel method for low-cost manufacturing of magnesium fluoride micro spheres from Baltic seawater was studied. The method focuses on a novel strategy of utilizing seawater. This study will facilitate large-scale production of MgF2 micro spheres. This method provides a prototype which can synthesize low-cost inorganic spheres from seawater.
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- Qin, Tao, 1984-, et al.
(författare)
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A novel rapid synthesis, characterization and applications of calcium phosphate nanospheres from Baltic seawater
- 2018
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Ingår i: Ceramics International. - : ELSEVIER SCI LTD. - 0272-8842 .- 1873-3956. ; 44:8, s. 9076-9079
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Tidskriftsartikel (refereegranskat)abstract
- Due to the considerable high bio-compatibility, calcium phosphate nanoparticles are widely used in biomedical applications. This study proposes a novel strategy for low-cost manufacturing calcium phosphate nanoporous spheres. The controlled reaction only took less than five minutes, when using Baltic seawater with the dissolved calcium concentration of 2.2 mM as the calcium resources. Porous nanospheres were obtained, with spheres ranging from 50 to 130 nm. The obtained nanospheres possess high drug-loading capacity and exhibit sustained release and pH-dependent properties. In addition, this method provides a general efficient strategy to synthesize other low-cost inorganic nanospheres from seawater.
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- Qin, Weiwei, et al.
(författare)
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Hydrogen bond-assisted synthesis of MoS2/reduced graphene oxide composite with excellent electrochemical performances for lithium and sodium storage
- 2018
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Ingår i: Journal of Colloid and Interface Science. - : ACADEMIC PRESS INC ELSEVIER SCIENCE. - 0021-9797 .- 1095-7103. ; 512, s. 826-833
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Tidskriftsartikel (refereegranskat)abstract
- MoS2/reduced graphene oxide composites (MoS2/rGO) were successfully prepared by a designed tris (hydroxymethyl)methyl aminomethane (named THAM)-assisted hydrothermal method, which involves the modification of THAM on the surfaces of graphene oxide via hydrogen bonds and then the adsorption of MoO42- on the decorated surfaces due to the electrostatic attraction. The three-dimensional framework of interconnected rGO nanosheets provides good electronic conductivity and facile strain release during the electrochemical reaction, thus enhancing the overall performance of the MoS2-based electrode. Herein, the composite delivers high specific capacity, excellent cycling stability and rate performance for lithium- and sodium-ions batteries (LIBs and SIBs). The MoS2/rGO anode exhibits capacities of 880 mAh g(-1) at 1 A g(-1) after 200 cycles and 396 mAh g(-1) even at 2 A g(-1) after 2000 cycles for LIBs. As to SIBS, the reversible capacities of 485 mAh g(-1) and 339 mAh g(-1) can be retained at 0.1 A g(-1) after 60 cycles and 0.5 A g(-1) after 300 cycles, respectively. Our results demonstrate that the MoS2/rGO anode is one of the attractive anodes for LIBs and SIBS. Furthermore, the facile method can be extended to biosensing, catalytic, and biomedical applications.
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| 10. |
- Aad, G, et al.
(författare)
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- 2015
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swepub:Mat__t
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