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- Yindee, M., et al.
(författare)
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Follicular Dynamics and Oestrous Detection in Thai Postpartum Swamp Buffaloes (Bubalus bubalis)
- 2011
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Ingår i: Reproduction in domestic animals. - : Blackwell Verlag. - 0936-6768 .- 1439-0531. ; 46:1, s. e91-e96
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Tidskriftsartikel (refereegranskat)abstract
- This study characterized follicular activity and oestrous behaviour from 5 to 9 days post-calving up to the 4th ovulation postpartum (pp) in 16 multiparous (range 2-7 parities) Thai swamp buffalo cows (Bubalus bubalis), aged 4-12 years and weighing from 432 to 676 kg. Ovarian follicular activity was examined by transrectal ultrasonography (TUS) every morning. Oestrous detection was performed twice daily by direct personal observation of behaviour and for presence of clear cervical mucus discharge and indirectly by video camera recording during 21 h/day. A follicular wave-like pattern was present before the 1st ovulation leading to short oestrous cycles. Growth rates and maximum diameters of the ovulatory follicles did not differ between the 1st and 4th ovulations. However, growth rate for non-ovulatory dominant follicles (DF) before the 1st ovulation was lower than for the ovulatory follicle (p < 0.05). In addition, the diameter of all ovulatory follicles (14.3 +/- 0.46 mm, n = 39) was significantly larger (p < 0.01) than those of the preceding last but one non-ovulatory DF (10.8 +/- 0.20 mm, n = 5), but similar to the last preceding non-ovulatory DF diameter (12.92 +/- 0.96 mm, n = 14). Short oestrous cycles were most common between the 1st and 2nd ovulations (93.75%, 15/16 cows, 10.2 +/- 0.38 days) decreasing in prevalence thereafter (50%, 3/6 buffaloes, 12.0 +/- 1.53 days). Oestrous signs were relatively vague around the 1st ovulation pp to become more easily detectable thereafter. This study suggests that properly fed swamp buffaloes could be mated successfully within 2 months pp, at their 2nd spontaneous ovulation, provided oestrous detection is at least performed daily at 06:00-08:00 hour.
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| 2. |
- Buranaamnuay, K., et al.
(författare)
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Effects of Straw Volume and Equex-STM (R) on Boar Sperm Quality after Cryopreservation
- 2009
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Ingår i: Reproduction in domestic animals. - : Wiley-Blackwell. - 0936-6768 .- 1439-0531. ; 44:1, s. 69-73
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Tidskriftsartikel (refereegranskat)abstract
- The present experiments were designed to study the effect of adding the detergent Equex-STM (R) to freezing extender, and of straw volume (0.25 ml vs 0.5 ml), on boar sperm quality after cryopreservation. Three ejaculates from each of four purebred boars (three Landrace and one Yorkshire) were collected and frozen with a lactose-egg yolk extender containing glycerol with or without 1.5% Equex-STM (R). The extended semen was loaded into either 0.25- or 0.5-ml straws. The straws were placed in liquid nitrogen (LN2) vapour approximately 3 cm above the level of LN2 for 20 min and then were plunged into LN2. Thawing was achieved in warm water at 50 degrees C for 12 s and then was incubated in a 38 degrees C water-bath for 30 min before evaluating sperm quality. Results showed that the individual motility, viability and acrosomal normal apical ridge (NAR) were improved (p less than 0.001) when Equex-STM (R) was added to the freezing extender. There was no difference (p = 0.48) in sperm motility between 0.25- and 0.5-ml straws when Equex-STM (R) was added. The percentages of viable and of NAR sperm in 0.5-ml straws were higher than those in 0.25-ml straws (p = 0.02, p = 0.0003 respectively). The percentages of membrane intact sperm evaluated using the short hypo-osmotic swelling test were not affected by straw volume or the adding of Equex-STM (R) (p greater than 0.05). The results of these investigations suggested that Equex-STM (R) exerts a beneficial effect on the quality of cryopreserved boar semen and this cryopreservation protocol was favourable for a 0.5-ml straw.
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